10 research outputs found
Resposta imune-humoral de búfalos (Bubalus bubalis) contra Anaplasma marginale (Theiler, 1910)
Short communication. Enhancement of the immune responses to vaccination against foot-and-mouth disease in mice by oral administration of Quillaja saponaria-A and extracts of Cochinchina momordica seed
This study was designed to evaluate the effects of oral administration of extracts from Cochinchina momordica seed
(ECMS) or Quillaja saponaria-A (Quil-A) on the immune responses in mice immunized with foot and mouth disease
virus (FMDV)-serotype O vaccine. Forty-two imprinting control region (ICR) mice were randomly divided into seven
groups of 6 animals in each group, and a dose of 400 ìg of Quil-A or ECMS was orally administered for 1, 2 or 3 days.
After that, the animals were subcutaneously immunized twice with FMD vaccine at 3-week intervals and blood samples
were collected 2-weeks after boosting for measurement of FMDV-specific IgG and its subclasses. Spleens were collected
for lymphocytes proliferation assay. Results indicated that serum FMDV-specific IgG and the IgG subclass responses
were significantly enhanced in mice orally administered ECMS or Quil-A when compared with the control group (p < 0.05).
Lymphocytes proliferation response to FMD vaccine was significantly enhanced by ECMS compared with the control
(p < 0.05). This study illustrates that ECMS induced immunomodulatory effects and performed better than Quil-A
Enhancement of immune responses to infectious bursal disease vaccine by supplement of an extract made from Momordica cochinchinensis (Lour.) Spreng. seeds
Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C*
The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice. Spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA). After 72-h culture, Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached. Spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression. Ginsenosides (1.0~10 μg/ml) significantly stimulated proliferation of spermatogonia. Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10−8 to 10−7 mol/L and the PKC inhibitor H7 inhibited this effect. Likewise, ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H7. These results indicate that the proliferating effect of ginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway