Project Exploration: 10-year Retrospective Program Evaluation

Assesses the impact of a program giving low-income students of color hands-on science experience on science capacity, youth development, and engagement in communities of practice. Examines practices that support science learning by underrepresented youth

Bioproduction of L-piperazic acid in gram scale using Aureobasidium melanogenum

Currently, piperazic acid is chemically synthesized using ecologically unfriendly processes. Microbial synthesis from glucose is an attractive alternative to chemical synthesis. In this study, we report the production of L-piperazic acid via microbial fermentation with the first engineered fungal strain of Aureobasidium melanogenum; this strain was constructed by chassis development, genetic element reconstitution and optimization, synthetic rewiring and constitutive genetic circuit reconstitution, to build a robust L-piperazic acid synthetic cascade. These genetic modifications enable A. melanogenum to directly convert glucose to L-piperazic acid without relying on the use of either chemically synthesized precursors or harsh conditions. This bio-based process overcomes the shortcomings of the conventional synthesis routes. The ultimately engineered strain is a very high-efficient cell factory that can excrete 1.12 ± 0.05 g l-1 of L-piperazic acid after a 120-h 10.0-l fed-batch fermentation; this is the highest titre of L-piperazic acid reported using a microbial cell factory

Uncovering Hierarchical Structure in Social Networks using Isospectral Reductions

We employ the recently developed theory of isospectral network reductions to analyze multi-mode social networks. This procedure allows us to uncover the hierarchical structure of the networks we consider as well as the hierarchical structure of each mode of the network. Additionally, by performing a dynamical analysis of these networks we are able to analyze the evolution of their structure allowing us to find a number of other network features. We apply both of these approaches to the Southern Women Data Set, one of the most studied social networks and demonstrate that these techniques provide new information, which complements previous findings.Comment: 17 pages, 5 figures, 5 table

Selective Refinement Network for High Performance Face Detection

High performance face detection remains a very challenging problem, especially when there exists many tiny faces. This paper presents a novel single-shot face detector, named Selective Refinement Network (SRN), which introduces novel two-step classification and regression operations selectively into an anchor-based face detector to reduce false positives and improve location accuracy simultaneously. In particular, the SRN consists of two modules: the Selective Two-step Classification (STC) module and the Selective Two-step Regression (STR) module. The STC aims to filter out most simple negative anchors from low level detection layers to reduce the search space for the subsequent classifier, while the STR is designed to coarsely adjust the locations and sizes of anchors from high level detection layers to provide better initialization for the subsequent regressor. Moreover, we design a Receptive Field Enhancement (RFE) block to provide more diverse receptive field, which helps to better capture faces in some extreme poses. As a consequence, the proposed SRN detector achieves state-of-the-art performance on all the widely used face detection benchmarks, including AFW, PASCAL face, FDDB, and WIDER FACE datasets. Codes will be released to facilitate further studies on the face detection problem.Comment: The first two authors have equal contributions. Corresponding author: Shifeng Zhang ([email protected]

Loss prevention for hog farmers: Insurance, on-farm biosecurity practices, and vaccination

Using agricultural household survey data and claim records from insurers for the year 2009, this paper analyzes hog producers' choice of means of loss prevention and identifies the relationships among biosecurity practices, vaccination, and hog insurance. By combining one probit and two structural equations, we adopt three-stage estimations on a mixed-process model to obtain the results. The findings indicate that biosecurity practices provide the basic infrastructure for operating pig farms and complement both the usage of quality vaccines and the uptake of hog insurance. In addition, there is a strong relationship of substitution between quality of vaccine and demand for hog insurance. Hog farmers that implement better biosecurity practices are more likely to seek high-quality vaccines or buy into hog insurance schemes but not both. For those households with hog insurance, better biosecurity status, better management practices, and higher-quality vaccine significantly help to reduce loss ratios. However, we also find a moral hazard effect in that higher premium expenditure by the insured households might induce larger loss ratios.Biosecurity, hog insurance, loss prevention, vaccine,

Extraction and optimization of B-spline PBD templates for recognition of connected handwritten digit strings

2001-2002 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Antiviral treatment alters the frequency of activating and inhibitory receptor-expressing natural killer cells in chronic Hepatitis B virus infected patients

Natural killer (NK) cells play a critical role in innate antiviral immunity, but little is known about the impact of antiviral therapy on the frequency of NK cell subsets. To this aim, we performed this longitudinal study to examine the dynamic changes of the frequency of different subsets of NK cells in CHB patients after initiation of tenofovir or adefovir therapy. We found that NK cell numbers and subset distribution differ between CHB patients and normal subjects; furthermore, the association was found between ALT level and CD158b+ NK cell in HBV patients. In tenofovir group, the frequency of NK cells increased during the treatment accompanied by downregulated expression of NKG2A and KIR2DL3. In adefovir group, NK cell numbers did not differ during the treatment, but also accompanied by downregulated expression of NKG2A and KIR2DL3. Our results demonstrate that treatment with tenofovir leads to viral load reduction, and correlated with NK cell frequencies in peripheral blood of chronic hepatitis B virus infection. In addition, treatments with both tenofovir and adefovir in chronic HBV infected patients induce a decrease of the frequency of inhibitory receptor+ NK cells, which may account for the partial restoration of the function of NK cells in peripheral blood following treatment

Malaria parasite translocon structure and mechanism of effector export.

The putative Plasmodium translocon of exported proteins (PTEX) is essential for transport of malarial effector proteins across a parasite-encasing vacuolar membrane into host erythrocytes, but the mechanism of this process remains unknown. Here we show that PTEX is a bona fide translocon by determining structures of the PTEX core complex at near-atomic resolution using cryo-electron microscopy. We isolated the endogenous PTEX core complex containing EXP2, PTEX150 and HSP101 from Plasmodium falciparum in the 'engaged' and 'resetting' states of endogenous cargo translocation using epitope tags inserted using the CRISPR-Cas9 system. In the structures, EXP2 and PTEX150 interdigitate to form a static, funnel-shaped pseudo-seven-fold-symmetric protein-conducting channel spanning the vacuolar membrane. The spiral-shaped AAA+ HSP101 hexamer is tethered above this funnel, and undergoes pronounced compaction that allows three of six tyrosine-bearing pore loops lining the HSP101 channel to dissociate from the cargo, resetting the translocon for the next threading cycle. Our work reveals the mechanism of P. falciparum effector export, and will inform structure-based design of drugs targeting this unique translocon

Automatically extracted Antarctic coastline using remotely-sensed data: an update

The temporal and spatial variability of the Antarctic coastline is a clear indicator of change in extent and mass balance of ice sheets and shelves. In this study, the Canny edge detector was utilized to automatically extract high-resolution information of the Antarctic coastline for 2005, 2010, and 2017, based on optical and microwave satellite data. In order to improve the accuracy of the extracted coastlines, we developed the Canny algorithm by automatically calculating the local low and high thresholds via the intensity histogram of each image to derive thresholds to distinguish ice sheet from water. A visual comparison between extracted coastlines and mosaics from remote sensing images shows good agreement. In addition, comparing manually extracted coastline, based on prior knowledge, the accuracy of planimetric position of automated extraction is better than two pixels of Landsat images (30 m resolution). Our study shows that the percentage of deviation (7 km2 (2005) to 1.3537 × 107 km2 (2010) and 1.3657 × 107 km2 (2017). We have found that the decline of the Antarctic area between 2005 and 2010 is related to the breakup of some individual ice shelves, mainly in the Antarctic Peninsula and off East Antarctica. We present a detailed analysis of the temporal and spatial change of coastline and area change for the six ice shelves that exhibited the largest change in the last decade. The largest area change (a loss of 4836 km2) occurred at the Wilkins Ice Shelf between 2005 and 2010