Zebrafish hoxd4a Acts Upstream of meis1.1 to Direct Vasculogenesis, Angiogenesis and Hematopoiesis

10.1371/journal.pone.0058857PLoS ONE83

Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation

Live imaging and genetic studies of the initial interactions between leukocytes and transformed cells in zebrafish larvae indicate an attractant role for H2O2 and suggest that blocking these early interactions reduces expansion of transformed cell clones

Excimer Laser Surgery: Biometrical Iris Eye Recognition with Cyclorotational Control Eye Tracker System

A prospective comparative study assessing the importance of the intra-operative dynamic rotational tracking—especially in the treatment of astigmatisms in corneal refractive Excimer laser correction—concerning clinical outcomes is presented. The cyclotorsion from upright to supine position was measured using iris image comparison. The Group 1 of patients was additionally treated with cyclorotational control and Group 2 only with X-Y control. Significant differences were observed between the groups regarding the mean postoperative cylinder refraction (p < 0.05). The mean cyclotorsion can be calculated to 3.75° with a standard deviation of 3.1°. The total range of torsion was from −14.9° to +12.6°. Re-treatment rate was 2.2% in Group 1 and 8.2% in Group 2, which is highly significant (p < 0.01). The investigation confirms that the dynamic rotational tracking system used for LASIK results in highly predictable refraction quality with significantly less postoperative re-treatments

Femtosecond laser-assisted in situ keratomileusis multifocal ablation profile using a mini-monovision approach for presbyopic patients with hyperopia

Iraklis Vastardis,1 Brigitte Pajic-Eggsp&uuml;hler,1 J&ouml;rg M&uuml;ller,1,2 Zeljka Cvejic,2 Bojan Pajic,1&ndash;4 1Swiss Eye Research Foundation, Orasis Eye Clinic, Aargau, Reinach, Switzerland; 2Faculty of Physics, University of Novi Sad, Novi Sad, Serbia; 3Department of Ophthalmology, Geneva University Hospitals, Geneva, Switzerland; 4Medical Faculty, Military Medical Academy, University of Defence, Belgrade, Serbia Purpose: To report the visual outcomes of the femtosecond laser-assisted multifocal aspheric corneal ablation profile using a mini-monovision approach and to evaluate if corneal multifocality was effective, and to report the relative benefits of this approach.Patients and methods: Bilateral femtosecond laser-assisted in situ keratomileusis using a multifocal aspheric corneal ablation profile was performed on 19 hyperopic patients (38 eyes). They were divided into two groups based on eye dominance: dominant eye (DE) group targeting emmetropia and the nondominant eye (NDE) group targeting -0.5&nbsp;D slight myopia. The uncorrected distance visual acuity (UDVA), uncorrected intermediate visual acuity (UIVA), uncorrected near visual acuity (UNVA), and retreatment rates were reported from baseline to 6&nbsp;months.Results: The UNVA, UIVA, and UDVA improved significantly in both groups (Kruskal&ndash;Wallis test, DE and NDE: P&lt;0.00001, P&lt;0.000005, and P=0.00001, respectively). Corrected distance visual acuity (CDVA) baseline was better in both groups in comparison to UDVA at 6&nbsp;months (Wilcoxon test, DE: P&lt;0.001, 95% confidence interval (CI) of the median 0.0&ndash;0.0 LogMAR and 0.1000&ndash;0.1218 LogMAR and NDE: P=0.010, 95% CI of the median 0.0&ndash;0.0 LogMAR and 0.00&ndash;0.10 LogMAR). There was a significant loss of lines between CDVA baseline and UDVA at 6&nbsp;months in both groups (DE group: 68% of eyes lost one line or more; NDE group: 58% of eyes lost one line or more). The corrected near visual acuity baseline compared to UNVA at 6&nbsp;months was not statistically important (Wilcoxon test, DE: P=0.8125, 95% CI of the median 0.0&ndash;0.0 LogMAR and 0.0&ndash;0.0 LogMAR and NDE: P=0.82, 95% CI of the median 0.0&ndash;0.0 LogMAR and 0.0&ndash;0.0 LogMAR). The comparison among the UDVA, UIVA, and UNVA between the two groups at baseline and during all follow-ups was not statistically important. Two cases from the DE group were retreated (6%).Conclusion: Use of this multifocal aspheric corneal ablation profile in patients with hyperopic presbyopia significantly improved UDVA, UIVA, and UNVA. This improvement was due to created multifocality of the cornea. The mini-monovision seems not to affect UDVA, UIVA, and UNVA between the two groups. The retreatment rates at the 6-month evaluation were significantly less in our study when compared with other studies. This method seems to improve UDVA, UIVA, and UNVA but could result in a significant statistical difference between CDVA baseline and UDVA at 6&nbsp;months that leads to loss of lines in distance vision. Despite promising results, this is a preliminary evaluation of this new profile, and a larger number of eyes are needed to verify visual outcomes, retreatment rates, and safety. Keywords: presbyopia, hyperopia, Femto LASIK, presbyLasik, visual acuity&nbsp

The Negation Bias in Stereotype Maintenance: A Replication in Five Languages

Research on linguistic biases shows that stereotypic expectancies are implicitly reflected in language and thereby subtly communicated to message recipients. Research on the Negation Bias shows that the use of negations (e.g., not stupid vs. smart) is more pronounced in descriptions of stereotype-inconsistent compared to stereotype-consistent behaviors. This paper reports a replication study of the original research conducted in Dutch (Beukeboom, Finkenauer, &amp; Wigboldus, 2010, study 2), using newly developed materials, and in five different languages: English, Dutch, Hungarian, Finnish, and Serbian. The results validate the existence of the Negation Bias in all five languages. This suggests that negation use serves a similar stereotype-maintaining function across language families

A mathematical approach to human pterygium shape

Bojan Pajic,1&ndash;4 Iraklis Vastardis,1 Predrag Rajkovic,5 Brigitte Pajic-Eggspuehler,1 Daniel M Aebersold,6 Zeljka Cvejic2 1Eye Clinic ORASIS, Swiss Eye Research Foundation, Reinach AG, Switzerland; 2Department of Physics, Faculty of Sciences, University of Novi Sad, Novi Sad,3Faculty of Medicine of the Military Medical Academy, University of Defence, Belgrade, Serbia; 4Division of Ophthalmology, Department of Clinical Neurosciences, Geneva University Hospitals, Geneva, Switzerland; 5Department of Mathematics, Faculty of Mechanical Engineering, University of Ni&scaron;, Ni&scaron;, Serbia; 6Department of Radiation Oncology, Inselspital, Bern University Hospital, Bern, Switzerland Purpose: Pterygium is a common lesion affecting the population in countries with high levels of ultraviolet exposure. The final shape of a pterygium is the result of a growth pattern, which remains poorly understood. This manuscript provides a mathematical analysis as a tool to determine the shape of human pterygia.Materials and methods: Eighteen patients, all affected by nasal unilateral pterygia, were randomly selected from our patient database independently of sex, origin, or race. We included all primary or recurrent pterygia with signs of proliferation, dry eye, and induction of astigmatism. Pseudopterygia were excluded from this study. Pterygia were outlined and analyzed mathematically using a Cartesian coordinate system with two axes (X, Y) and five accurate landmarks of the pterygium.Results: In 13 patients (72%), the shape of the pterygia was hyperbolic and in five patients (28%), the shape was rather elliptical.Conclusion: This analysis gives a highly accurate mathematical description of the shape of human pterygia. This might help to better assess the clinical results and outcome of the great variety of therapeutic approaches concerning these lesions. Keywords: etiology, pterygium, limbal stem cells, stem cells dysfunction, mathematical shape analysi

The health and well-being of Australia's future medical doctors: Protocol for a 5-year observational cohort study of medical trainees

Introduction Clinical training in the undergraduate medical course places multiple stressors on trainees, which have been held to lead to heightened distress, depression, suicide, substance misuse/abuse and poor mental health outcomes. To date, evidence for morbidity in trainees is largely derived from cross-sectional survey-based research. This limits the accuracy of estimates and the extent to which predispositional vulnerabilities (biological and/or psychological), contextual triggers and longer-term consequences can be validly identified. Longitudinal clinical assessments embedded within a biopsychosocial framework are needed before effective preventative and treatment strategies can be put in place. Methods and analysis This study is an observational longitudinal cohort study of 330 students enrolled in the undergraduate medicine course at the University of New South Wales (UNSW) Sydney, Australia. Students will be recruited in their fourth year of study and undergo annual assessments for 4 consecutive years as they progress through increasingly demanding clinical training, including internship. Assessments will include clinical interviews for psychiatric morbidity, and self-report questionnaires to obtain health, psychosocial, performance and functioning information. Objective measures of cognitive performance, sleep/activity patterns as well as autonomic and immune function (via peripheral blood samples) will be obtained. These data will be used to determine the prevalence, incidence and severity of mental disorder, elucidate contextual and biological triggers and mechanisms underpinning psychopathology and examine the impact of psychopathology on performance and professional functioning. Ethics and dissemination Ethics approval has been granted by the UNSW human research ethics committee (reference HC16340). The findings will be disseminated through peer-reviewed publications and conference presentations, and distributed to key stakeholders within the medical education sector. The outcomes will also inform targeted preventative and treatment strategies to enhance stress resilience in trainee doctors

Influence of heavy rare earth ions substitution on microstructure and magnetism of nanocrystalline magnetite

In this work we report results on the influence of heavy rare earth ions substitution on microstructure and magnetism of nanocrystalline magnetite. A series of Fe(2.85)RE(0.15)O(4) (RE = Gd, Dy, Ho, Tm and Yb) samples have been prepared by high energy ball milling. Structure/microstructure investigations of two selected samples Fe(2.85)Gd(0.15)O(4) and Fe(2.85)Tm(0.15)O(4), represent an extension of the previously published results on Fe(3)O(4)/gamma-Fe(2)O(3), Fe(2.85)Y(0.15)O(4) and Fe(2.55)In(0.45)O(4) [Z. Cvejic, S. Rakic, A. Kremenovic, B. Antic, C. Jovalekic. Ph. Colomban, Sol. State Sciences 8 (2006) 908], while magnetic characterization has been done for all the samples. Crystallite/particle size and strain determined by X-ray diffractometry and Transmission electron microscopy (TEM) confirmed the nanostructured nature of the mechanosynthesized materials. X-ray powder diffraction was used to analyze anisotropic line broadening effects through the Rietveld method. The size anisotropy was found to be small while strain anisotropy was large, indicating nonuniform distribution of deffects in the presence of Gd and Tm in the crystal structure. Superparamagnetic(SPM) behavior at room temperature was observed for all samples studied. The Y-substituted Fe(3)O(4) had the largest He and the lowest M(S). We discuss the changes in magnetic properties in relation to their magnetic anisotropy and microstructure. High field irreversibility (H GT 20kOe) in ZFC/FC magnetization versus temperature indicates the existence of high magnetocrystalline and/or strain induced anisotropy. (C) 2008 Elsevier B.V. All rights reserved