3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei

Background: Fluorescent hybridization techniques are widely used to study the functional organization of different compartments within the mammalian nucleus. However, few examples of such studies are known in the plant kingdom. Indeed, preservation of nuclei 3D structure, which is required for nuclear organization studies, is difficult to fulfill. Results: We report a rapid protocol for fluorescent in situ hybridization (FISH) performed on 3D isolated nuclei and thin cryosectioned leaves of Arabidopsis thaliana. The use of direct labeling minimized treatment steps, shortening the overall procedure. Using image analysis, we measured different parameters related to nucleus morphology and overall 3D structure. Conclusion: Our work describes a 3D-FISH protocol that preserves the 3D structure of Arabidopsis interphase nuclei. Moreover, we report for the first time FISH using cryosections of Arabidopsis leaves. This protocol is a valuable tool to investigate nuclear architecture and chromatin organization

Reuse of medical face masks in domestic and community settings without sacrificing safety: Ecological and economical lessons from the Covid-19 pandemic

The need for personal protective equipment increased exponentially in response to the Covid-19 pandemic. To cope with the mask shortage during springtime 2020, a French consortium was created to find ways to reuse medical and respiratory masks in healthcare departments. The consortium addressed the complex context of the balance between cleaning medical masks in a way that maintains their safety and functionality for reuse, with the environmental advantage to manage medical disposable waste despite the current mask designation as single-use by the regulatory frameworks. We report a Workflow that provides a quantitative basis to determine the safety and efficacy of a medical mask that is decontaminated for reuse. The type IIR polypropylene medical masks can be washed up to 10 times, washed 5 times and autoclaved 5 times, or washed then sterilized with radiations or ethylene oxide, without any degradation of their filtration or breathability properties. There is loss of the antiprojection properties. The Workflow rendered the medical masks to comply to the AFNOR S76-001 standard as “type 1 non-sanitory usage masks”. This qualification gives a legal status to the Workflow-treated masks and allows recommendation for the reuse of washed medical masks by the general population, with the significant public health advantage of providing better protection than cloth-tissue masks. Additionally, such a legal status provides a basis to perform a clinical trial to test the masks in real conditions, with full compliance with EN 14683 norm, for collective reuse. The rational reuse of medical mask and their end-of-life management is critical, particularly in pandemic periods when decisive turns can be taken. The reuse of masks in the general population, in industries, or in hospitals (but not for surgery) has significant advantages for the management of waste without degrading the safety of individuals wearing reused masks

Dissolution spécifique à la périphérie des Grands Causses français

This paper deals with the results of the specific erosion evident on the carbonate formations, on the western border of the Great Causses of France : Can de l'Hospitalet, Can de Tardonenche, le Lempezou, Causse des Bonbons, Valdonnez basin. The results are obtained form the hydrochemical measurements taken form the hydrological cycles of 1982-1983. These results are then compared with those of other local and regional karsts. They show that there is marked erosion, linked to a well developped endokarst. This situation contrasts with a deficiency of exokarstic pheno¬ mena.A partir de mesures hydrochimiques effectuées sur les formations carbonatées de la bordure orientale des Grands Causses, nous donnons des valeurs repères de la dissolution spécifique que nous comparons à d'autres zones d'études.Maurin Yves. Dissolution spécifique à la périphérie des Grands Causses français. In: Karstologia : revue de karstologie et de spéléologie physique, n°14, 2e semestre 1989. pp. 43-46

Recherches sur le creusement et la stabilité des grands volumes karstiques souterrains. Thèse de 3e cycle présentée par Eric Gilli, soutenue le 25 mai 1984 à l'Université de Provence - Aix Marseille

Maurin Yves. Recherches sur le creusement et la stabilité des grands volumes karstiques souterrains. Thèse de 3e cycle présentée par Eric Gilli, soutenue le 25 mai 1984 à l'Université de Provence - Aix Marseille. In: Karstologia : revue de karstologie et de spéléologie physique, n°6, 2e semestre 1985. pp. 61-62

Recherches sur le creusement et la stabilité des grands volumes karstiques souterrains. Thèse de 3e cycle présentée par Eric Gilli, soutenue le 25 mai 1984 à l'Université de Provence - Aix Marseille

Maurin Yves. Recherches sur le creusement et la stabilité des grands volumes karstiques souterrains. Thèse de 3e cycle présentée par Eric Gilli, soutenue le 25 mai 1984 à l'Université de Provence - Aix Marseille. In: Karstologia : revue de karstologie et de spéléologie physique, n°6, 2e semestre 1985. pp. 61-62

Fusion de modèles neuroanatomiques tridimensionnels

PARIS-BIUSJ-Thèses (751052125) / SudocPARIS-BIUSJ-Physique recherche (751052113) / SudocSudocFranceF

Le Droguier de la faculté de pharmacie de Montpellier (sauvegarde du patrimoine et intérêts d une de ses plantes : L Eleutherococcus senticosus)

MONTPELLIER-BU Pharmacie (341722105) / SudocSudocFranceF

L'alimentation des Guadeloupéens

MONTPELLIER-BU Pharmacie (341722105) / SudocSudocFranceF