In Vitro Studies of a New Radiosensitizer for Radiotherapy of Breast Cancer

Breast cancer is the most commonly diagnosed cancer among women and is the leading cause of cancer death in both developing and developed areas. Triple-negative breast cancer, one of the subtypes of breast cancer, is generally more aggressive and has fewer treatment options compared with other subtypes, due to the absence of drug-targetable receptors. Radiotherapy delivers ionizing radiation to cancerous areas, leading to DNA damage and cell death. There has been controversy about the molecular mechanism of DNA damage induced by ionizing radiation. According to conventional notions, biological damage by ionizing radiation is primarily induced by the hydroxyl radical, but Lu et al., using femtosecond time-resolved laser spectroscopy, have discovered that the dissociative electron transfer (DET) reaction of the weakly-bound prehydrated electron plays an important role in causing chemical bond and DNA strand breaks. Building on the better understanding of DET reaction mechanisms of prehydrated electron, the mechanism behind the radiosensitizing effect of cisplatin and halopyrimidines has been discovered by our group. However, cisplatin has severe toxicity, and the DET reaction efficiency of halopyrimidines is low. A newly discovered non-platinum-based compound, FMD-Br-DAB, identified through the femtomedicine approach, was tested in this research as a potential radiosensitizer. Our in vitro results have confirmed that FMD-Br-DAB can exert radiosensitizing effects on treated triple-negative breast cancer (MDA-MB-231) cells when combined with radiation. Our results also indicate that FMD-Br-DAB can enhance DNA damage in a dose-dependent manner when combined with radiation.1 yea

Ovarian tissue cryopreservation in the pediatric with rare diseases- experience from China’s first and the largest ovarian tissue cryobank

BackgroundThere is limited information about the efficacy of ovarian tissue cryopreservation (OTC) in children. In the present study, we report eight patients with rare diseases who underwent OTC in China’s first and largest ovarian tissue cryobank.ProcedureData from girls with rare diseases who underwent OTC between September 2020 and November 2022 were retrospectively analyzed. We also compared the number of cryopreserved cortex pieces, follicle number, and AMH in those with rare diseases and age-matched children with non-rare diseases who also underwent OTC in our cryobank.ResultsThe median age of the children was 5.88 ± 3.52 (range 2-13) years old. Unilateral oophorectomy was undertaken via laparoscopy in all of the children. The diseases in the 8 patients were: 4 mucopolysaccharidoses (MPS I two cases, IVA two cases), 1 Diamond-Blackfan anemia (DBA), 1 Fanconi anemia (FA), 1 hyperimmunoglobulin E syndrome (HIES), 1 Niemann-Pick disease. The number of cryopreserved cortex pieces was 17.13 ± 6.36, and the follicle count per 2 mm biopsy was 447.38 ± 524.35. No significant difference in age, the count of cryopreserved cortex pieces, follicle number per 2 mm biopsy, and AMH level was seen between the 20 children with non-rare diseases and those with rare diseases.ConclusionsThe reports help practitioners counsel girls with rare diseases about fertility preservation. The demand for OTC in pediatrics will likely grow as a standard of care

Synthesis of a new chromogenic reagent, 1-(<em>p</em>-azophenyl)-3-(2-thiazol) triazene, and its application to determination of zinc(II)

466-468Synthesis and characterization of a new chromogenic reagent, l-(p-azophenyl)-3-(2-thiazol)triazene(APTT), are described. The reagent has been used for spectrophotometric determination of zinc(II) APTT forms a red colored complex with zinc(II) in alkaline m dium in the presence of Triton X-l00. The optimum conditions for the colour reaction have been descried. The molar absorptivity of the complex is 9.6 X 104 1.moI-1 cm-1 at 545 nm. Beer's law is obeyed over the range 0 - 2 µg/25ml Zn(lI). lnterference due to several ions has been studied. The recommended method has been applied to the determination of zinc(II) in aluminum alloys

Changes in Protein Degradation and Non-Volatile Flavor Substances of Swimming Crab (Portunus trituberculatus) during Steaming

To investigate the effect of steaming time (0, 5, 10, 15, 20, and 25 min) on the protein degradation and non-volatile flavor substances of swimming crab (Portunus trituberculatus), the moisture content, total nitrogen (TN), non-protein nitrogen (NPN), free amino acids (FAAs), flavor nucleotides, electronic tongue analysis, and sensory evaluation were determined. The results showed that the contents of NPN and total FAAs were the highest after crabs steamed for 10 min. Meanwhile, the AMP (adenosine monophosphate) content reached the maximum value (332.83 mg/100 g) and the taste active value (TAV) reached 6.67, which indicated that AMP contributes the most to the taste of steamed crab at 10 min. The electronic tongue distinguished the taste difference well, and the sensory score was the highest at 15 min. Combined with equivalent umami concentration (EUC) and TAV value, swimming crab (weight = 200 &plusmn; 20 g) steamed for 10&ndash;15 min tasted best

Study on spatial-temporal change of soil organic carbon and impact factors in Gudao Forestry Farm of Dongying

Soil organic carbon (SOC) is one of the important properties to evaluate soil quality, which can indirectly reflect the effect of afforestation on saline-alkali land. In this study, 4 different forest types in Gudao Forestry Centre were selected as experimental plots, and bare land was set as blank group. Spatial differentiation and impact factors of SOC between different forest types were studied by field investigation and laboratory experiment. The results show that (1) SOC content in the study area is lower than 1%. SOC content in forest land is higher than that in bare land. In the upper soil layer, the SOC content of Robinia Pseudoacacia forest is lower, and mixed forest is higher. SOC content of bare land is higher than that of forest land in the lower layer. (2) SOC has a significant positive correlation with available nutrients (P<0.05), and has a weak correlation with pH and water content. There are different correlations with conductivity and bulk density in different soil layers, and they all showed a negative correlation at the upper layer, and a significant positive correlation at the lower layer (P<0.05)