Practical thermodynamics of Yukawa systems at strong coupling

Simple practical approach to estimate thermodynamic properties of strongly coupled Yukawa systems, in both fluid and solid phases, is presented. The accuracy of the approach is tested by extensive comparison with direct computer simulation results (for fluids and solids) and the recently proposed shortest-graph method (for solids). Possible applications to other systems of softly repulsive particles are briefly discussed.Comment: Published in J. Chem. Phy

Effect of plasma resonances on dynamic characteristics of double graphene-layer optical modulator

We analyze the dynamic operation of an optical modulator based on double graphene-layer(GL) structure utilizing the variation of the GL absorption due to the electrically controlled Pauli blocking effect. The developed device model yields the dependences of the modulation depth on the control voltage and the modulation frequency. The excitation of plasma oscillations in double-GL structure can result in the resonant increase of the modulation depth, when the modulation frequency approaches the plasma frequency, which corresponds to the terahertz frequency for the typical parameter values.Comment: 8 pages, 4 figure

Expression of Tumor Assosiated and Epithelial-mesenchymal Transition Markers in 2d and 3d Cell Cultures of Mcf-7

The target effects on the expression of epithelial-mesenchymal transition regulation molecules are promising for cancer therapy, including breast cancer. 3D cell culture is a model for studying epithelial-mesenchymal transition in vitro and may become a test system for anticancer therapy.Aim of research. The aim of this research was to evaluate and compare the expression of tumor associated and epithelial-mesenchymal transition markers in tumor cells of breast adenocarcinoma (MCF-7 cell line) in 2D and 3D cell culture.Methods. For realization of the aim MCF-7 cell line (breast adenocarcinoma) was chosen as an experimental model in vitro. The monolayer cell culture was cultured in standard conditions (37 0C, 5 % CO2, humidity 95 %). The initial density of inoculated cells was 2 x 104 cells/cm2. The cells were incubated for two days before their use in the experiment. For the initial generation of spheroids the monolayer cell culture was removed off the substrate after the four days of incubation, using 0,25 % Trypsin-EDTA, and placed in nutrient medium with 5 % carboxymethyl cellulose (Bio-Rad, USA) at concentration of 5 x 105 cells/ml. Then the plates were incubated on an orbital shaker (Orbital shaker, PSU-10i, Biosan, Latvia) at 50 rpm for 3–5 hours. Half of culture medium was replenished every 3 days. A spheroid culture was maintained for 14 days. Detection of markers (ER, p53, EpCAM, vim, AE1/AE3, panCK, EGFR) in 2D and 3D cell culture was performed using immunohistochemistry method with primary monoclonal antibodies. Histological samples of cells were photographed to compare the morphological characteristics and the expression of proteins in monolayer and spheroid cultureResults. The results demonstrated that the percentage of tumor marker positive cells (ER+, EGFR+, EpCAM+, panCK+, AE1/AE3+) in monolayer culture is 1.25–2 times than more in spheroid culture. In contrast, tumor spheroids consist of fewer cells with the expression of epithelial markers such as EpCAM and AE1/AE3, but they contain a large number of cells that expressed mesenchymal marker vimentin by 5 % and p53 by 10 %. This may indicate that the cells acquire a mesenchymal phenotype. However, tumor cells of monolayer cell culture were not expressed vimentin.Conclusions. Our results demonstrated the differences of expression of tumor associated and epithelial-mesenchymal transition markers in 2D and 3D breast cancer cell cultures. Thus, the percentage of epithelial markers (Cytokeratines and epithelial cell adhesion molecule) in tumor spheroids is less than in cells of monolayer however spheroids cells begin expressing a mesenchymal marker – vimentin. In 3D cell culture only the outer cell layers expressed tumor associated proteins unlike 2D cell culture in which all of cells showed equally expression. Reduced of manifestation of tumor associated markers in 3D cell culture may indicate an increase of stem properties. These data showed that 3D cell culture more than 2D cell culture characterized processes of epithelial-mesenchymal transition

The multilevel regulation of CD95 signaling outcome

CD95, also called Fas or APO-1, was the first death receptor (DR) identified and characterized. Studies on CD95 receptor signaling revealed the versatile principles of cell fate regulation via DR. DRs could exert both pro- and anti-apoptotic effects depending on clustering, internalization or signaling thresholds and other extracellular signals. It became clear that molecular network regulating cell death and survival is under the multilevel control. In this Review we focus on the regulation of CD95 signaling and provide brief analysis of molecular switches of its pro- and antiapoptotic functions. At least five levels of life-death cell regulation via CD95 could be tracked: extracellular, membrane, DISC, mitochondrial, and miRNA. The cellular outcome of signaling via DRs depends on other extracellular signals and availability of different intracellular components of signal transduction pathways. This article is part of a Special Issue entitled “Apoptosis: Four Decades Later”

УПРАВЛІННЯ ПРОЦЕСОМ ОЦІНЮВАННЯ ЗНАНЬ ПРИ ПІДГОТОВЦІ РЯ- ТУВАЛЬНИКІВ З ВИКОРИСТАННЯМ ОНТОЛОГІЇ НАВЧАЛЬНИХ КУРСІВ

In connection with the expansion of distance education there is a necessity of the development and use of computer-aided learning and knowledge control. The main attention is paid to the peculiarities of software development and the problems of optimization of the educational material structure, and the development of a methodology of knowledge testing and methods of verification remain on the sidelines. This article proposes a new approach to the development of automated control systems of knowledge based on ontology of subject areas, which in this case are the training courses. The formation of ontology and logic scheme of control of knowledge allows structuring the learning material, to identify topics and issues that represent challenges for the cadets (students), as well as to develop a software wrapper for uniform formation of control systems of knowledge of various training courses.У зв'язку з поширенням дистанційної освіти виникає необхідність розробки і використання засобів автоматизованого навчання і контролю знань. При цьому головну увагу звертають на особливості розробки програмного забезпечення, а проблеми оптимізації структури навчального матеріалу, а також розробки методології тестування знань і методів її верифікації залишаються осторонь. Запропоновано новий підхід до розробки автоматизованих систем контролю знань, заснований на онтологіях предметних областей, якими в даному випадку є навчальні курси. Формування онтології та логічної схеми контролю знань дозволяє структурувати навчальний матеріал, визначити теми і питання, які становлять труднощі для курсантів (студентів), а також розробити програмну оболонку для уніфікованого формування систем контролю знань з різних навчальних курсів

Flux Penetration in Superconducting Strip with Edge-Indentation

The flux penetration near a semicircular indentation at the edge of a thin superconducting strip placed in a transverse magnetic field is investigated. The flux front distortion due to the indentation is calculated numerically by solving the Maxwell equations with a highly nonlinear $E(j)$ law. We find that the excess penetration, $\Delta$, can be significantly ($\sim$ 50%) larger than the indentation radius $r_0$, in contrast to a bulk supercondutor in the critical state where $\Delta=r_0$. It is also shown that the flux creep tends to smoothen the flux front, i.e. reduce $\Delta$. The results are in very good agreement with magneto-optical studies of flux penetration into an YBa$_2$Cu$_3$O$_x$ film having an edge defect.Comment: 5 pages, 7 figure

A method for calculating temperature-dependent photodissociation cross sections and rates

The destruction of molecules by photodissociation plays a major role in many radiation-rich environments, including the evolution of the atmospheres of exoplanets, which often exist close to UV-rich stars. Most current photodissociation calculations and databases assume T = 0 K, which is inadequate for hot exoplanets and stars. A method is developed for computing photodissociation spectra of diatomic molecules as a function of temperature exploiting bound state variational nuclear motion program Duo and post-processing program ExoCross. Discrete transition intensities are spread out to represent a continuous photodissociation spectrum either by Gaussian smoothing or by averaging calculations over a range of different grid sizes. Our approach is tested on four different chemical species (HCl, HF, NaCl and BeH+), showing its ability to reproduce photodissociation cross sections and rates computed with other approaches and experiment. The temperature dependence of photodissociation cross sections and rates is studies showing strong temperature variation of the photodissociation cross sections

Low temperature scattering with the R-matrix method: the Morse potential

Experiments are starting to probe collisions and chemical reactions between atoms and molecules at ultra-low temperatures. We have developed a new theoretical procedure for studying these collisions using the R-matrix method. Here this method is tested for the atom -- atom collisions described by a Morse potential. Analytic solutions for continuum states of the Morse potential are derived and compared with numerical results computed using an R-matrix method where the inner region wavefunctions are obtained using a standard nuclear motion algorithm. Results are given for eigenphases and scattering lengths. Excellent agreement is obtained in all cases. Progress in developing a general procedure for treating ultra-low energy reactive and non-reactive collisions is discussed.Comment: 18 pages, 6 figures, 3 tables, conferenc