Hypomethylation of IL10 and IL13 Promoters in CD4+ T Cells of Patients with Systemic Lupus Erythematosus

Interleukin- (IL-)10 and IL-13 play important roles in Th2 cell differentiation and production of autoantibodies in patients with (SLE). However, the mechanisms leading to IL10 and IL13 overexpression in SLE patients are not well understood. In this study, we confirm that the levels of both IL10 and IL13 mRNA in CD4+ T cells and of serum IL10 and IL13 proteins are increased in SLE patients. We show that the DNA methylation levels within IL10 and IL13 gene regulatory domains are reduced in SLE CD4+ T cells relative to healthy controls and negatively correlate with IL10 and IL13 mRNA expression. Moreover, treating healthy CD4+ T cells with the demethylating agent 5-azacytidine (5-azaC) increased IL10 and IL13 mRNA transcription. Together, our results show that promoter methylation is a determinant of IL10 and IL13 expression in CD4+ T cells, and we propose that DNA hypomethylation leads to IL10 and IL13 overexpression in SLE patients

Assessing branching structure for biomass and wood quality estimation using terrestrial laser scanning point clouds

Terrestrial laser scanning (TLS) accompanied by quantitative tree-modeling algorithms can potentially acquire branching data non-destructively from a forest environment and aid the development and calibration of allometric crown biomass and wood quality equations for species and geographical regions with inadequate models. However, TLS's coverage in capturing individual branches still lacks evaluation. We acquired TLS data from 158 Scots pine (Pinus sylvestris L.) trees and investigated the performance of a quantitative branch detection and modeling approach for extracting key branching parameters, namely the number of branches, branch diameter (b(d)) and branch insertion angle (b) in various crown sections. We used manual point cloud measurements as references. The accuracy of quantitative branch detections decreased significantly above the live crown base height, principally due to the increasing scanner distance as opposed to occlusion effects caused by the foliage. b(d) was generally underestimated, when comparing to the manual reference, while b was estimated accurately: tree-specific biases were 0.89cm and 1.98 degrees, respectively. Our results indicate that full branching structure remains challenging to capture by TLS alone. Nevertheless, the retrievable branching parameters are potential inputs into allometric biomass and wood quality equations.Peer reviewe

Quantitative Assessment of Scots Pine (Pinus Sylvestris L.) Whorl Structure in a Forest Environment Using Terrestrial Laser Scanning

State-of-the-art technology available at sawmills enables measurements of whorl numbers and the maximum branch diameter for individual logs, but such information is currently unavailable at the wood procurement planning phase. The first step toward more detailed evaluation of standing timber is to introduce a method that produces similar wood quality indicators in standing forests as those currently used in sawmills. Our aim was to develop a quantitative method to detect and model branches from terrestrial laser scanning (TLS) point clouds data of trees in a forest environment. The test data were obtained from 158 Scots pines (Pinus sylvestris L.) in six mature forest stands. The method was evaluated for the accuracy of the following branch parameters: Number of whorls per tree and for every whorl, the maximum branch diameter and the branch insertion angle associated with it. The analysis concentrated on log-sections (stem diameter > 15 cm) where the branches most affect wood's value added. The quantitative whorl detection method had an accuracy of 69.9% and a 1.9% false positive rate. The estimates of the maximum branch diameters and the corresponding insertion angles for each whorl were underestimated by 0.34 cm (11.1%) and 0.67 degrees (1.0%), with a root-mean-squared error of 1.42 cm (46.0%) and 17.2 degrees (26.3%), respectively. Distance from the scanner, occlusion, and wind were the main external factors that affect the method's functionality. Thus, the completeness and point density of the data should be addressed when applying TLS point cloud based tree models to assess branch parameters.Peer reviewe

Variability of wood properties using airborne and terrestrial laser scanning

Information on wood properties is crucial in estimating wood quality and forest biomass and thus developing the precision and sustainability of forest management and use. However, wood properties are highly variable between and within trees due to the complexity of wood formation. Therefore, tree-specific field references and spatially transferable models are required to capture the variability of wood quality and forest biomass at multiple scales, entailing high-resolution terrestrial and aerial remote sensing methods. Here, we aimed at identifying select tree traits that indicate wood properties (i.e. wood quality indicators) with a combination of terrestrial laser scanning (TLS) and airborne laser scanning (ALS) in an examination of 27 even-aged, managed Scots pine (Pinus sylvestris L.) stands in southern Finland. We derived the wood quality indicators from tree models sampled systematically from TLS data and built prediction models with respect to individual crown features delineated from ALS data. The models were incapable of predicting explicit branching parameters (height of the lowest dead branch R2 = 0.25, maximum branch diameter R2 = 0.03) but were suited to predicting stem and crown dimensions from stand, tree, and competition factors (diameter at breast height and sawlog volume R2 = 0.5, and live crown base height R2 = 0.4). We were able to identify the effect of canopy closure on crown longevity and stem growth, which are pivotal to the variability of several wood properties in managed forests. We discussed how the fusions of high-resolution remote sensing methods may be used to enhance sustainable management and use of natural resources in the changing environment.Peer reviewe

Is field-measured tree height as reliable as believed A comparison study of tree height estimates from field measurement, airborne laser scanning and terrestrial laser scanning in a boreal forest

Quantitative comparisons of tree height observations from different sources are scarce due to the difficulties in effective sampling. In this study, the reliability and robustness of tree height observations obtained via a conventional field inventory, airborne laser scanning (ALS) and terrestrial laser scanning (TLS) were investigated. A carefully designed non-destructive experiment was conducted that included 1174 individual trees in 18 sample plots (32 m x 32 m) in a Scandinavian boreal forest. The point density of the ALS data was approximately 450 points/m(2). The TLS data were acquired with multi-scans from the center and the four quadrant directions of the sample plots. Both the ALS and TLS data represented the cutting edge point cloud products. Tree heights were manually measured from the ALS and TLS point clouds with the aid of existing tree maps. Therefore, the evaluation results revealed the capacities of the applied laser scanning (LS) data while excluding the influence of data processing approach such as the individual tree detection. The reliability and robustness of different tree height sources were evaluated through a cross-comparison of the ALS-, TLS-, and field- based tree heights. Compared to ALS and TLS, field measurements were more sensitive to stand complexity, crown classes, and species. Overall, field measurements tend to overestimate height of tall trees, especially tall trees in codominant crown class. In dense stands, high uncertainties also exist in the field measured heights for small trees in intermediate and suppressed crown class. The ALS-based tree height estimates were robust across all stand conditions. The taller the tree, the more reliable was the ALS-based tree height. The highest uncertainty in ALS-based tree heights came from trees in intermediate crown class, due to the difficulty of identifying treetops. When using TLS, reliable tree heights can be expected for trees lower than 15-20 m in height, depending on the complexity of forest stands. The advantage of LS systems was the robustness of the geometric accuracy of the data. The greatest challenges of the LS techniques in measuring individual tree heights lie in the occlusion effects, which lead to omissions of trees in intermediate and suppressed crown classes in ALS data and incomplete crowns of tall trees in TLS data.Peer reviewe

Seamless integration of above- and under-canopy unmanned aerial vehicle laser scanning for forest investigation

BackgroundCurrent automated forest investigation is facing a dilemma over how to achieve high tree- and plot-level completeness while maintaining a high cost and labor efficiency. This study tackles the challenge by exploring a new concept that enables an efficient fusion of aerial and terrestrial perspectives for digitizing and characterizing individual trees in forests through an Unmanned Aerial Vehicle (UAV) that flies above and under canopies in a single operation. The advantage of such concept is that the aerial perspective from the above-canopy UAV and the terrestrial perspective from the under-canopy UAV can be seamlessly integrated in one flight, thus grants the access to simultaneous high completeness, high efficiency, and low cost.ResultsIn the experiment, an approximately 0.5ha forest was covered in ca. 10min from takeoff to landing. The GNSS-IMU based positioning supports a geometric accuracy of the produced point cloud that is equivalent to that of the mobile mapping systems, which leads to a 2-4cm RMSE of the diameter at the breast height estimates, and a 4-7cm RMSE of the stem curve estimates.ConclusionsResults of the experiment suggested that the integrated flight is capable of combining the high completeness of upper canopies from the above-canopy perspective and the high completeness of stems from the terrestrial perspective. Thus, it is a solution to combine the advantages of the terrestrial static, the mobile, and the above-canopy UAV observations, which is a promising step forward to achieve a fully autonomous in situ forest inventory. Future studies should be aimed to further improve the platform positioning, and to automatize the UAV operation.Peer reviewe

Hypomethylation of IL10 and IL13 Promoters in CD4 + T Cells of Patients with Systemic Lupus Erythematosus

Interleukin-(IL-)10 and IL-13 play important roles in Th2 cell differentiation and production of autoantibodies in patients with (SLE). However, the mechanisms leading to IL10 and IL13 overexpression in SLE patients are not well understood. In this study, we confirm that the levels of both IL10 and IL13 mRNA in CD4 + T cells and of serum IL10 and IL13 proteins are increased in SLE patients. We show that the DNA methylation levels within IL10 and IL13 gene regulatory domains are reduced in SLE CD4 + T cells relative to healthy controls and negatively correlate with IL10 and IL13 mRNA expression. Moreover, treating healthy CD4 + T cells with the demethylating agent 5-azacytidine (5-azaC) increased IL10 and IL13 mRNA transcription. Together, our results show that promoter methylation is a determinant of IL10 and IL13 expression in CD4 + T cells, and we propose that DNA hypomethylation leads to IL10 and IL13 overexpression in SLE patients

Can we early diagnose metabolic syndrome using brachial-ankle pulse wave velocity in community population

BACKGROUND: The prevalence of metabolic syndrome (MetS) increased recently and there was still not a screening index to predict MetS. The aim of this study was to estimate whether brachial-ankle pulse wave velocity (baPWV), a novel marker for systemic arterial stiffness, could predict MetS in Chinese community population. METHODS: A total of 2 191 participants were recruited and underwent medical examination including 1 455 men and 756 women from June 2011 to January 2012. MetS was diagnosed according to the criteria of the International Diabetes Federation (IDF). Multiple Logistic regressions were conducted to explore the risk factors of MetS. Receiver operating characteristic (ROC) curve was performed to estimate the ideal diagnostic cutoff point of baPWV to predict MetS. RESULTS: The mean age was (45.35+/-8.27) years old. In multiple Logistic regression analysis, the gender, baPWV and smoking status were risk factors to MetS after adjusting age, gender, baPWV, walk time and sleeping time. The prevalence of MetS was 17.48% in 30-year age population in Shanghai. There were significant differences (chi(2) = 96.46, P \u3c 0.05) between male and female participants on MetS prevalence. According to the ROC analyses, the ideal cutoff point of baPWV was 1 358.50 cm/s (AUC = 60.20%) to predict MetS among male group and 1 350.00 cm/s (AUC = 70.90%) among female group. CONCLUSION: BaPWV may be considered as a screening marker to predict MetS in community Chinese population and the diagnostic value of 1 350.00 cm/s was more significant for the female group

International Benchmarking of the Individual Tree Detection Methods for Modeling 3-D Canopy Structure for Silviculture and Forest Ecology Using Airborne Laser Scanning

Canopy structure plays an essential role in biophysical activities in forest environments. However, quantitative descriptions of a 3-D canopy structure are extremely difficult because of the complexity and heterogeneity of forest systems. Airborne laser scanning (ALS) provides an opportunity to automatically measure a 3-D canopy structure in large areas. Compared with other point cloud technologies such as the image-based Structure from Motion, the power of ALS lies in its ability to penetrate canopies and depict subordinate trees. However, such capabilities have been poorly explored so far. In this paper, the potential of ALS-based approaches in depicting a 3-D canopy structure is explored in detail through an international benchmarking of five recently developed ALS-based individual tree detection (ITD) methods. For the first time, the results of the ITD methods are evaluated for each of four crown classes, i.e., dominant, codominant, intermediate, and suppressed trees, which provides insight toward understanding the current status of depicting a 3-D canopy structure using ITD methods, particularly with respect to their performances, potential, and challenges. This benchmarking study revealed that the canopy structure plays a considerable role in the detection accuracy of ITD methods, and its influence is even greater than that of the tree species as well as the species composition in a stand. The study also reveals the importance of utilizing the point cloud data for the detection of intermediate and suppressed trees. Different from what has been reported in previous studies, point density was found to be a highly influential factor in the performance of the methods that use point cloud data. Greater efforts should be invested in the point-based or hybrid ITD approaches to model the 3-D canopy structure and to further explore the potential of high-density and multiwavelengths ALS data