17 research outputs found
Ulcerative colitis associated with nephrotic syndrome after treatment with mesalazine developed into rectal carcinoma: a case study
Significant neutralizing activity of human immunoglobulin preparations against pandemic 2009 H1N1
Isolation of Sialyloligosaccharides from Egg Yolk Using Enzymes and Some Biofunctional Activities of the Oligosaccharides Isolated
Hepatic overexpression of a dominant negative form of raptor enhances Akt phosphorylation and restores insulin sensitivity in K/KAy mice
Significant neutralizing activity of human immunoglobulin preparations against pandemic 2009 H1N1
LST8 level controls basal p70 S6 kinase and Akt phosphorylations, and mTORC1 and mTORC2 negatively regulate each other by competing for association with LST8
Reliability of a Newly-Developed Immunochromatography Diagnostic Kit for Pandemic Influenza A/H1N1pdm Virus: Implications for Drug Administration
<div><h3>Background</h3><p>For the diagnosis of seasonal influenza, clinicians rely on point-of-care testing (POCT) using commercially available kits developed against seasonal influenza viruses. However, POCT has not yet been established for the diagnosis of pandemic influenza A virus (H1N1pdm) infection due to the low sensitivity of the existing kits for H1N1pdm.</p> <h3>Methodology/Principal Findings</h3><p>An immunochromatography (IC) test kit was developed based on a monoclonal antibody against H1N1pdm, which does not cross-react with seasonal influenza A or B viruses. The efficacy of this kit (PDM-IC kit) for the diagnosis of H1N1pdm infection was compared with that of an existing kit for the detection of seasonal influenza viruses (SEA-IC kit). Nasal swabs (n = 542) were obtained from patients with flu-like syndrome at 13 clinics in Osaka, Japan during the winter of 2010/2011. Among the 542 samples, randomly selected 332 were further evaluated for viral presence by reverse transcriptase polymerase chain reaction (RT-PCR). The PDM-IC kit versus the SEA-IC kit showed higher sensitivity to and specificity for H1N1pdm, despite several inconsistencies between the two kits or between the kits and RT-PCR. Consequently, greater numbers of false-negative and false-positive cases were documented when the SEA-IC kit was employed. Significant correlation coefficients for sensitivity, specificity, and negative prediction values between the two kits were observed at individual clinics, indicating that the results could be affected by clinic-related techniques for sampling and kit handling. Importantly, many patients (especially influenza-negative cases) were prescribed anti-influenza drugs that were incongruous with their condition, largely due to physician preference for patient responses to questionnaires and patient symptomology, as opposed to actual viral presence.</p> <h3>Conclusions/Significance</h3><p>Concomitant use of SEA-IC and PDM-IC kits increased the likelihood of correct influenza diagnosis. Increasing the credibility of POCT is anticipated to decrease the inappropriate dispensing of anti-influenza drugs, thereby minimizing the emergence of drug-resistant H1N1pdm strains.</p> </div
Effect of patient age and duration from disease onset to collection of samples on rapid test kit results.
<p>The SEA-IC test kit (A, C) and the PDM-IC test kit (B, D) were independently evaluated for sensitivity (blue), specificity (red), PPV (green), and NPV (purple) according to patient age (from <10 to ≥60 years old) (A, B) and duration from disease onset to sampling (1, 2, and ≥3 days) (C, D). P values in (C) indicate significant differences between groups. The 332 samples with information regarding patient age were used for (A) and (B), and the 321 samples with information regarding the number of days after disease onset were used for (C) and (D).</p