An α-synuclein decoy peptide prevents cytotoxic α-synuclein aggregation caused by fatty acid binding protein 3

α-synuclein (αSyn) is a protein known to form intracellular aggregates during the manifestation of Parkinson’s disease. Previously, it was shown that αSyn aggregation was strongly suppressed in the midbrain region of mice that did not possess the gene encoding the lipid transport protein fatty acid binding protein 3 (FABP3). An interaction between these two proteins was detected in vitro, suggesting that FABP3 may play a role in the aggregation and deposition of αSyn in neurons. In order to characterize the molecular mechanisms that underlie the interactions between FABP3 and αSyn that modulate the cellular accumulation of the latter, in this report, we used in vitro fluorescence assays combined with fluorescence microscopy, transmission electron microscopy, and quartz crystal microbalance assays to characterize in detail the process and consequences of FABP3-αSyn interaction. We demonstrated that binding of FABP3 to αSyn results in changes in the aggregation mechanism of the latter; specifically, a suppression of fibrillar forms of αSyn, and also the production of aggregates with an enhanced cytotoxicity toward mice neuro2A cells. Since this interaction involved the C-terminal sequence region of αSyn, we tested a peptide derived from this region of αSyn (αSynP130-140) as a decoy to prevent the FABP3-αSyn interaction. We observed that the peptide competitively inhibited binding of αSyn to FABP3 in vitro and in cultured cells. We propose that administration of αSynP130-140 might be used to prevent the accumulation of toxic FABP3-αSyn oligomers in cells, thereby preventing the progression of Parkinson’s disease

Host selection of hematophagous leeches (Haemadipsa japonica): Implications for iDNA studies

The development of an efficient and cost‐effective method for monitoring animal populations or biodiversity is urgently needed, and invertebrate‐derived DNA (iDNA) may offer a promising tool for assessing the diversity and other ecological information of vertebrates. We studied the host species of a hematophagous leech (Haemadipsa japonica) in Yakushima by genetic barcoding and compared the results with those for mammal composition revealed by camera trapping. We analyzed 119 samples using two sets of primers by Sanger sequencing and one set of primer by next generation sequencing. The proportion of the samples that were successfully sequenced and identified to at least one species was 11.8–24.3%, depending on the three different methods. In all of these three methods, most of the samples were identified as sika deer (18/20, 6/15 and 16/29) or human (2/20, 7/15 and 21/29). The nonhuman mammal host species composition was significantly different from that estimated by camera trapping. Sika deer was the main host, which may be related with their high abundance, large body size and terrestriality. Ten samples included DNA derived from multiple species of vertebrates. This may be due to the contamination of human DNA, but we also found DNA from deer, Japanese macaque and a frog in the same samples, suggesting the mixture of the two meals in the gut of the leech. Using H. japonica‐derived iDNA would not be suitable to make an inventory of species, but it may be useful to collect genetic information on the targeted species, due to their high host selectivity

Paleoclimatic and paleoceanographic records through Marine Isotope Stage 19 at the Chiba composite section, central Japan: A key reference for the EarlyeMiddle Pleistocene Subseries boundary

Marine Isotope Stage (MIS) 19 is an important analogue for the present interglacial because of its similar orbital configuration, especially the phasing of the obliquity maximum to precession minimum. However, sedimentary records suitable for capturing both terrestrial and marine environmental changes are limited, and thus the climatic forcing mechanisms for MIS 19 are still largely unknown. The Chiba composite section, east-central Japanese archipelago, is a continuous and expanded marine sedimentary succession well suited to capture terrestrial and marine environmental changes through MIS 19. In this study, a detailed oxygen isotope chronology is established from late MIS 20 to early MIS 18, supported by a U-Pb zircon age and the presence of the Matuyama–Brunhes boundary. New pollen, marine microfossil, and planktonic foraminiferal δ18O and Mg/Ca paleotemperature records reveal the complex interplay of climatic influences. Our pollen data suggest that the duration of full interglacial conditions during MIS 19 extends from 785.0 to 775.1 ka (9.9 kyr), which offers an important natural baseline in predicting the duration of the present interglacial. A Younger Dryas-type cooling event is present during Termination IX, suggesting that such events are linked to this orbital configuration. Millennial- to multi-millennial-scale variations in our δ18O and Mg/Ca records imply that the Subarctic Front fluctuated in the northwestern Pacific Ocean during late MIS 19, probably in response to East Asian winter monsoon variability. The climatic setting at this time appears to be related to less severe summer insolation minima at 65˚N and/or high winter insolation at 50˚N. Our records do not support a recently hypothesized direct coupling between variations in the geomagnetic field intensity and global/regional climate change. Our highly resolved paleoclimatic and paleoceanographic records, coupled with a well-defined Matuyama–Brunhes boundary (772.9 ka; duration 1.9 kyr), establish the Chiba composite section as an exceptional climatic and chronological reference section for the Early–Middle Pleistocene boundary.ArticleQuaternary Science Reviews 191: 406-430(2018)journal articl

Measuring Space Efficiency and Estimating the Potential for Reduced Operational and Embodied Energy Use for Office Spaces

This paper explores how opportunities for reducing the total use of office space can be identified, investigates how the benefits in terms of energy savings from space efficiency measures could be calculated, and gives a first estimate of such values. A simple method to measure office space use is presented and tested at two university departments, and very low space efficiency is found. A variety of reasons for the low space efficiency are identified via interviews with property managers and heads of the concerned departments. These include the fact that the incentives for using space efficiently are small for the decision-makers, and the costs in terms of time and trouble are perceived as high. This suggests that interesting results can be achieved without large efforts. Moreover, we present a proof of concept of how to estimate the amount of energy that can be saved by reducing space use. We find a rough estimate of the potential energy savings of 2 MWh/m2 in embodied primary energy intensity (assuming that more efficient use of space leads to a decrease in new construction) and 200 kWh/m2/year in final energy intensity. Those numbers should be useful as rough estimates when looking at opportunities for saving energy by using space more efficiently.</p

Transplanting Date Estimation Using Sentinel-1 Satellite Data for Paddy Rice Damage Assessment in Indonesia

In Indonesia, there is a need to improve the efficiency of damage assessments of the agricultural insurance system for paddy rice producers affected by floods, droughts, pests, and diseases. In this study, we develop a method to estimate the transplanting date required for damage assessments of paddy rice fields. The study area is the Cihea irrigation district in West Java, Republic of Indonesia. Backscattering coefficients of VH polarization measured by a synthetic aperture radar onboard the Sentinel-1 satellite were used for the estimations. We investigated the accuracy of the estimations of the proposed method by smoothing out the time-series data, applying a speckle filter, and by signal synthesis of the surrounding fields. It was found that these variations effectively improved the estimation accuracy. To further improve the estimation accuracy, the data for all incident angles were used after correcting the incident angle dependence of the backscattering coefficients for three types of data with different incident angles (32&deg;, 41&deg;, and 45&deg;) obtained in the study area. The estimated transplanting date for each field in the test site was compared with the transplanting date obtained through interviews. The standard deviations of the estimation errors for the four cropping periods from March 2018 to February 2020 were found to be ~5&ndash;6 days, and the percentages of estimation errors in transplanting dates within 5, 10, and 15 days were estimated to be 69%, 92%, and 97%, respectively. It was confirmed that a sufficiently reliable transplanting date estimation can be obtained ~10&ndash;15 days after transplantation