Determination of 17 β-estradiol in pharmaceutical preparation by UV spectrophotometry and high performance liquid chromatography methods

In this study, new, rapid UV spectrophotometry (UV) and reversed phase high performance liquid chromatography (HPLC) methods were developed for the determination of 17 β-estradiol in pure and in pharmaceutical dosage form. The solvent system, wavelength of detection and chromatographic conditions were optimized in order to maximize the sensitivity of both the proposed methods. The linear regression equations obtained by least square regression method were y = 0.0184x + 0.0059 for the UV method and y = 56742x − 3403.6 for the HPLC method. The developed methods were successfully employed with a high degree of precision and accuracy for the estimation of total drug content in a commercial tablet of 17 β-estradiol. The results obtained from the UV method were compared with those obtained by using HPLC. The proposed methods are highly sensitive, precise and accurate and can be used for the reliable quantitation of 17 β-estradiol in pharmaceutical dosage form

Determination of neopterin in urine of industrial workers by HPLC

Abstract This study aimed to determine neopterin levels in the urine of industrial workers by the high-performance liquid chromatography method. Intra- and inter-day precision values for neopterin in urine were less than 3.14, and accuracy (relative error) was better than 3.00%. The limits of detection and quantification of neopterin were 0.3 and 1.0 ng/mL, respectively. Also, the developed method was applied to real samples to determine the neopterin levels in the urines of industrial workers, who have been exposed to various chemicals such as formaldehyde, heavy metals and thinners. Urine neopterin levels of industrial workers including auto painters, bodywork and furniture workers were statistically compared with healthy volunteers. The highest and lowest values of urinary neopterin for industrial workers were obtained 908.96 and 119.86 μmol/mol, respectively. Our investigation demonstrates that there is a meaningful difference in urinary neopterin levels between the workers and the control groups (P<0.05). Workers in the auto paint, body and furniture business may have been exposed to a toxic environmental exposure in their occupation. As a result, an increase in the concentration of neopterin in the urine may be important in the diagnosis and treatment of various diseases

The effect of surgical technique on hemodynamics, arterial oxygenation and pulmonary mechanics in radical prostatectomy operations

Objective: The effects of surgical technique on respiratory mechanics, arterial oxygenation and hemodynamics in radical prostatectomy operation were investigated. Methods: The study was planned on ASA II-III, 40-65 years old, fourty patients scheduled for radical prostatectomy under general anesthesia. They were divided into two groups: perineal and suprapubic (Group P, n = 20; Group S, n = 20). Heart rate, mean arterial blood pressure, arterial oxygen saturation (SpO2), partial pressure of end-tidal carbon dioxide (PEtCO2), Peak inspiratory pressure (PIP), plato pressure (Pplato), partial pressure of oxygen in arterial blood (PaO2), partial pressure of carbon dioxide in arterial blood (PaCO2) values were evaluated at 10 minutes after induction. After the position applied for surgery in the 30.60 and 90th minutes, the Alveolar-arterial oxygen pressure gradient (P(A-a) O2), the ratio of physiologic dead space over tidal volume (VD/VT), arterial to end tidal CO2 gradient (P(a-et) CO2), static compliance (CS), dynamic compliance (CD) were assessed. Results: In the assessment of groups, there were not statistical differences about mean blood pressure, heart rate, SpO2, PetCO2, PaO2, plateau pressure, and P (A-a) values (p &gt; 0.05). Peak inspiratory pressure was higher in Group P. Peak inspiratory pressure and plateau pressure increased with CO2 insufflation in Group P. PaCO2 and P(a-et) CO2 were higher statistically significantly in Group 0. There was no difference in terms of the PetCO2 values. VD/VT ratios were statistically significantly lower in the Group P. Conclusions: Suprapubic surgery was shown to improve oxygenation and respiratory mechanics without causing any hemodynamic side effect in radical prostatectomy operation

The effect of N-acetylcysteine on inflammation and oxidative stress in cisplatin-induced nephrotoxicity: a rat model

Background/Aim: Cisplatin is a highly effective chemotherapeutic agent used in the treatment of solid organ cancers. Besides its chemotherapeutic effectiveness, cisplatin administration is associated with numerous side effects. Of those, the most clinically significant and common effect is nephrotoxicity. Recent studies reported that oxidative stress and inflammation are probably the most important mechanisms that contribute to the nephrotoxicity. N-acetylcysteine (NAC) is an antioxidant and antiinflammatory agent. In the present study, the effects of NAC on cisplatin-induced nephrotoxicity were investigated

Bio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigations

WOS: 000341335800036PubMed ID: 25009101A novel and efficient approach for the preparation of enriched herbal formulations was described and their potential applications including wound healing and antioxidant activity (cell based and cell free) were investigated via in vitro cell culture studies. Nigella sativa oil was enriched with Calendula officinalis extract and lipoic acid capped gold nanoparticles (AuNP-LA) using nanoemulsion systems. The combination of these bio-active compounds was used to design oil in water (O/W) and water in oil (W/O) emulsions. The resulted emulsions were characterized by particle size measurements. The phenolic content of each nanoemulsion was examined by using both colorimetric assay and chromatographic analyses. Two different methods containing cell free chemical assay (1-diphenyl-2-picrylhydrazyl method) and cell based antioxidant activity test were used to evaluate the antioxidant capacities. In order to investigate the bio-activities of the herbal formulations, in vitro cell culture experiments, including cytotoxicity, scratch assay, antioxidant activity and cell proliferation were carried out using Vero cell line as a model cell line. Furthermore, to monitor localization of the nanoemulsions after application of the cell culture, the cell images were monitored via fluorescence microscope after FITC labeling. All data confirmed that the enriched N. sativa formulations exhibited better antioxidant and wound healing activity than N. sativa emulsion without any enrichment. In conclusion, the incorporation of AuNP-LA and C officinalis extract into the N. sativa emulsions significantly increased the bio-activities. The present work may support further studies about using the other bio-active agents for the enrichment of herbal preparations to strengthen their activities. (C) 2014 Elsevier B.V. All rights reserved.State Planning Organization (DPT) of Turkey [0395.STZ.2013-2]; Ege UniversityEge University [2013/FEN/023]; Ege University Aliye Uster FoundationEge UniversityThis work was financially supported by the State Planning Organization (DPT) of Turkey through the project no 0395.STZ.2013-2. It was partially supported by Ege University Scientific Research Project (2013/FEN/023) and Ege University Aliye Uster Foundation. SEM (Izmir) was also acknowledged for their support during the HPLC analysis

Serum Angiopoietin-like peptide 4 levels in patients with hepatic steatosis

Objectives: Angiopoietin-like peptide 4 (ANGPTL-4) plays an important role in lipid metabolism by inhibiting the enzyme lipoprotein lipase. This effect of ANGPTL-4 results in suppression of the release of plasma triglyceri-dederived fatty acids. Increase in fatty acid levels entering to the liver and abnormalities in their secretion is one of the main mechanisms in pathogenesis of hepatic steatosis. In this study, we aimed to investigate the role of ANGPTL-4 in pathogenesis of hepatic steatosis by determining its levels in patients with fatty liver disease