Dataset-Katano_et_al_RSOS

The all data used in the paper, grazer (M. quadriloba larvae) density and chlorophyll (chl) a of periphyton in the stream

Fig. S1 Abiotic factors (water temperature, electric conductivity (EC), and precipitation) at each survey station in the Shigo-gawa stream during downstream surveys. Precipitation was observed at Nara Meteorological Observatory. Table S1. Environmental variables (mean ± 1 SD) of each sampling site. The values in parentheses are sample sizes. from Distribution and drift dispersal dynamics of a caddisfly grazer in response to resource abundance and its ontogeny

Stream grazers have a major impact on food web structure and the productivity of stream ecosystems; however, studies on the longitudinal (upstream versus downstream) and temporal changes in their drift dynamics and resulting distributions remain limited. Here, we investigated the longitudinal and temporal distributions and drift propensity of a trichopteran grazer, the caddisfly, <i>Micrasema quadriloba</i>, during its life cycle in a Japanese stream. The distribution of larvae significantly shifted downstream during the fifth instar larval stage during late winter; with periphyton abundance (i.e. their food source) showing similar shifts downstream. Therefore, our results show that the drift dispersal the caddisfly occurs in response to decline in available food resources (i.e. food-resource scarcity) and an increase in food requirements by growing individuals. Furthermore, our results show that this observed longitudinal shift in larval distribution varies through their life cycle, because the drift dispersal of fifth instar larvae was greater than that of immature larvae. The correlation between periphyton abundance and drift propensity of fourth instar larvae was not statistically significant, whereas that of fifth instar larvae was significantly negative. In conclusion, we detected an ontogenetic shift in drift propensity, which might explain the longitudinal and temporal distributions of this species

Diagnosis for joint symptoms at baseline and endpoint.

<p>Diagnosis at baseline is based upon the American College of Rheumatology / European League against Rheumatism (ACR/EULAR) classification criteria for RA in 2010. Diagnosis of RA at endpoint is based upon primary rheumatologists’ diagnosis. Diagnosis at endpoint is based upon the primary rheumatologists’ diagnosis. The diagnosises in non RA→non RA group was Sjogren syndrome (3), Pseudogout (2), Polymyalgia rheumatic (1), Tenosynovitis (1), and Undifferentiated arthritis (22). MTX; methotrexate treatment introduction, PD; diagnosis of periodontitis (maximal probing depth≧4mm), Pg; presence of <i>Porphyromolas gingivalis</i> in subgingival biofilm. Categorical variables were expressed as percentages (numbers) and were analyzed by Pearson’s qui squared test. P values for the different PD status groups at baseline are 0.36 (RA→RA vs non RA→non RA), 0.23 (RA→RA vs non RA→RA), 0.04 (non RA→RA vs non RA→non RA), respectively. P values for different Pg status groups at baseline are 0.37 (RA→RA vs non RA→non RA), 0.44 (RA→RA vs non RA→RA), 0.04 (non RA→RA vs non RA→non RA), respectively.</p><p>Diagnosis for joint symptoms at baseline and endpoint.</p

Multivariate analysis of covariance for factors associated simplified disease activity index of rheumatoid arthritis.

<p>Multivariate analysis of covariance was used to analyze the effect size of the clinical diagnosis of periodontitis based upon the 5th European Workshop in Periodontology (EWP) in 2005 (0: no periodontitis, 1: moderate periodontitis, 2: severe periodontitis) and the presence of <i>P</i>. <i>gingivalis</i> for disease activity of rheumatoid arthritis after adjusting for age, sex, smoking status, frequency of tooth brushing per a day. Disease activity of rheumatoid arthritis was evaluated by simplified disease activity index. SDAI; simplified disease activity index, P; P value, ES; Effect size</p><p>*; p<0.05</p><p>Multivariate analysis of covariance for factors associated simplified disease activity index of rheumatoid arthritis.</p

Hazard ratio for methotrexate treatment introduction during the follow up period.

<p>The patients were divided into two groups based upon the diagnosis of PD (maximal probing depth ≧ 4 mm) <b>(a)</b>, three groups based upon the severity of PD (EWP) <b>(b)</b>, or two groups based upon the presence of <i>P</i>. <i>gingivalis</i> in subgingival biofilm <b>(c)</b>. MTX treatment introduction in each groups were compared by cumulative hazard method. X axis indicates days for MTX treatment introduction after the first visit. Y axis indicates the cumulative hazard ratio for MTX introduction. Hazard ratio of positive PD (probing depth≧4mm) vs negative PD was 2.68 (95% CI, 1.11–6.50), p = 0.03. Hazard ratio of severe PD (EWP) vs non PD (EWP) was 7.26 (95% CI, 0.75–69.9), p = 0.09, and hazard ratio of moderate PD vs non PD was 4.42 (95% CI, 0.60–32.4), p = 0.15. Hazard ratio of positive Pg vs negative Pg was 0.77 (95% CI, 0.39–1.53), p = 0.45.</p

Characteristics of the patients divided by the clinical diagnosis of periodontitis or the presence of <i>Porphyromonas gingivalis</i> in subgingival biofilm.

<p>Continuous variables were expressed as mean ± SD. The difference of continuous variables between two groups were analyzed by students’ t-test and those among three groups were analyzed by Kruskal-Wallis test. Categorical variables were expressed as percentages (numbers). The difference of categorical variables between two groups was analyzed by Pearson’s qui-squared test and those among three groups were analyzed by Kruskal-Wallis test. EWP: periodontitis classification criteria of the 5th European Workshop in Periodontology in 2005; SDAI: simplified disease activity index; mHAQ: modified health assessment questionnaire; N.D.: not determined; N.A.: not assessed</p><p>*: p<0.05 Pg was not analyzed in one patient because DNA of subgingival plaque sample was not successfully extracted.</p><p>Characteristics of the patients divided by the clinical diagnosis of periodontitis or the presence of <i>Porphyromonas gingivalis</i> in subgingival biofilm.</p

Baseline characteristics of the study patients.

<p>Continuous variables were expressed as mean ± SD. Categorical variables were expressed as % (number). EWP: periodontitis classification criteria of the 5^th European Workshop in Periodontology in 2005, SDAI: simplified disease activity index; mHAQ: modified health assessment questionnaire</p><p>Baseline characteristics of the study patients.</p