Sparing brain damage in severe sepsis: a beginning

Sepsis-associated encephalopathy (SAE) resembles metabolic encephalopathies but with a difference: there is the potential for enduring brain damage/dysfunction. The pathogenesis of SAE is likely multifactorial. However, the severity of SAE parallels the severity of the septic illness and the brain's microcirculation is probably affected in a similar manner to that of other organs. Mild cases of SAE are often completely reversible, but there is increasing evidence that severe cases have neurological sequelae. A better understanding of the mechanisms may lead to brain-sparing, protective strategies

Non-functional biomimicry : utilising natural patterns to provoke attention responses

Natural reoccurring patterns arise from chaos and are prevalent throughout nature. The formation of these patterns is controlled by, or produces, underlying geometrical structures. Biomimicry is the study of nature’s structure, processes and systems, as models and solutions for design challenges and is being widely utilized in order to address many issues of contemporary engineering. Many academics now believe that aesthetics stem from pattern recognition, consequently, aesthetic preference may be a result of individuals recognising, and interacting with, natural patterns. The goal of this research was to investigate the impact of specific naturally occurring pattern types (spiral, branching, and fractal patterns) on user behaviour; investigating the potential of such patterns to control and influence how individuals interact with their surrounding environment. The results showed that the underlying geometry of natural patterns has the potential to induce attention responses to a statistically significant level

De novo formation of an aggregation pheromone precursor by an isoprenyl diphosphate synthase-related terpene synthase in the harlequin bug.

Insects use a diverse array of specialized terpene metabolites as pheromones in intraspecific interactions. In contrast to plants and microbes, which employ enzymes called terpene synthases (TPSs) to synthesize terpene metabolites, limited information from few species is available about the enzymatic mechanisms underlying terpene pheromone biosynthesis in insects. Several stink bugs (Hemiptera: Pentatomidae), among them severe agricultural pests, release 15-carbon sesquiterpenes with a bisabolene skeleton as sex or aggregation pheromones. The harlequin bug, Murgantia histrionica, a specialist pest of crucifers, uses two stereoisomers of 10,11-epoxy-1-bisabolen-3-ol as a male-released aggregation pheromone called murgantiol. We show that MhTPS (MhIDS-1), an enzyme unrelated to plant and microbial TPSs but with similarity to trans-isoprenyl diphosphate synthases (IDS) of the core terpene biosynthetic pathway, catalyzes the formation of (1S,6S,7R)-1,10-bisaboladien-1-ol (sesquipiperitol) as a terpene intermediate in murgantiol biosynthesis. Sesquipiperitol, a so-far-unknown compound in animals, also occurs in plants, indicating convergent evolution in the biosynthesis of this sesquiterpene. RNAi-mediated knockdown of MhTPS mRNA confirmed the role of MhTPS in murgantiol biosynthesis. MhTPS expression is highly specific to tissues lining the cuticle of the abdominal sternites of mature males. Phylogenetic analysis suggests that MhTPS is derived from a trans-IDS progenitor and diverged from bona fide trans-IDS proteins including MhIDS-2, which functions as an (E,E)-farnesyl diphosphate (FPP) synthase. Structure-guided mutagenesis revealed several residues critical to MhTPS and MhFPPS activity. The emergence of an IDS-like protein with TPS activity in M. histrionica demonstrates that de novo terpene biosynthesis evolved in the Hemiptera in an adaptation for intraspecific communication

Comparing the temperatures of galaxy clusters from hydro-N-body simulations to Chandra and XMM-Newton observations

Theoretical studies of the physical processes guiding the formation and evolution of galaxies and galaxy clusters in the X-ray are mainly based on the results of numerical hydrodynamical N-body simulations, which in turn are often directly compared to X-ray observations. Although trivial in principle, these comparisons are not always simple. We demonstrate that the projected spectroscopic temperature of thermally complex clusters obtained from X-ray observations is always lower than the emission-weighed temperature, which is widely used in the analysis of numerical simulations. We show that this temperature bias is mainly related to the fact that the emission-weighted temperature does not reflect the actual spectral properties of the observed source. This has important implications for the study of thermal structures in clusters, especially when strong temperature gradients, like shock fronts, are present. Because of this bias, in real observations shock fronts appear much weaker than what is predicted by emission-weighted temperature maps, and may even not be detected. This may explain why, although numerical simulations predict that shock fronts are a quite common feature in clusters of galaxies, to date there are very few observations of objects in which they are clearly seen. To fix this problem we propose a new formula, the spectroscopic-like temperature function, and show that, for temperature larger than 3 keV, it approximates the spectroscopic temperature better than few per cent, making simulations more directly comparable to observations.Comment: Submitted for publication in MNRAS; 15 pages, 10 color figures and 13 BW figures,mn2e.cls. High resolution figures available here: http://people.roma2.infn.it/~mazzotta/preprints/mazzotta.pd

Ethical considerations in functional magnetic resonance imaging research in acutely comatose patients

After severe brain injury, one of the key challenges for medical doctors is to determine the patient’s prognosis. Who will do well? Who will not do well? Physicians need to know this, and families need to do this too, to address choices regarding the continuation of life supporting therapies. However, current prognostication methods are insufficient to provide a reliable prognosis. Functional Magnetic Resonance Imaging (MRI) holds considerable promise for improving the accuracy of prognosis in acute brain injury patients. Nonetheless, research on functional MRI in the intensive care unit context is ethically challenging. These studies raise several ethical issues that have not been addressed so far. In this article, Prof. Charles Weijer and his co-workers provide a framework for researchers and ethics committees to design and review these studies in an ethically sound way

Seedbank Persistence of Palmer Amaranth (\u3ci\u3eAmaranthus palmeri\u3c/i\u3e) and Waterhemp (\u3ci\u3eAmaranthus tuberculatus\u3c/i\u3e) across Diverse Geographical Regions in the United States

Knowledge of the effects of burial depth and burial duration on seed viability and, consequently, seedbank persistence of Palmer amaranth (Amaranthus palmeri S. Watson) and waterhemp [Amaranthus tuberculatus (Moq.) J. D. Sauer] ecotypes can be used for the development of efficient weed management programs. This is of particular interest, given the great fecundity of both species and, consequently, their high seedbank replenishment potential. Seeds of both species collected from five different locations across the United States were investigated in seven states (sites) with different soil and climatic conditions. Seeds were placed at two depths (0 and 15cm) for 3 yr. Each year, seeds were retrieved, and seed damage (shrunken, malformed, or broken) plus losses (deteriorated and futile germination) and viability were evaluated. Greater seed damage plus loss averaged across seed origin, burial depth, and year was recorded for lots tested at Illinois (51.3% and 51.8%) followed by Tennessee (40.5% and 45.1%) and Missouri (39.2% and 42%) for A. palmeri and A. tuberculatus, respectively. The site differences for seed persistence were probably due to higher volumetric water content at these sites. Rates of seed demise were directly proportional to burial depth (α=0.001), whereas the percentage of viable seeds recovered after 36 mo on the soil surface ranged from 4.1% to 4.3% compared with 5% to 5.3% at the 15-cm depth for A. palmeri and A. tuberculatus, respectively. Seed viability loss was greater in the seeds placed on the soil surface compared with the buried seeds. The greatest influences on seed viability were burial conditions and time and site-specific soil conditions, more so than geographical location. Thus, management of these weed species should focus on reducing seed shattering, enhancing seed removal from the soil surface, or adjusting tillage systems

The weed community affects yield and quality of soybean (Glycine max (L.) Merr.)

The relationship between the weed community and soybean (Glycine max (L.) Merr.) seed yield and quality was assessed in two soybean experiments in Illinois, USA. One field was sown with different proportions of target weeds (Ambrosia trifida L., Amaranthus rudis J. Sauer, Setaria faberi F. Herrm), and the other was naturally infested with these and other weeds. The composition of the weed communities in both fields were compared to final yield and quality (% protein, oil, and water) of the crop using NMDS ordination. Biomass and canopy cover, and seed quality (% protein, relative water content, seed weight) of the crop, were related to the multivariate structure of the weed community in both experiments. Lower quality soybeans were harvested from plots dominated by the target weeds and a suite of subordinate volunteers. Analysis restricted to the volunteer weed community was also significantly related to seed protein and seed weight. Similar results from the two experiments lend generality to the findings and indicate that soybean producers need to manage the composition of the weed community