561 research outputs found
Screening for the optimal siRNA targeting a novel gene (HA117) and construction and evaluation of a derivative recombinant adenovirus
We found a novel gene named as HA117 in our previous research. At this study, we screened for an optimal siRNA targeting the novel gene HA117 using the pSOS-HUS method, verified the results of pSOS-HUS siRNA screening for optimal affinity for the target gene, and constructed and evaluated a recombinant adenovirus carrying the DNA template for transcription of the optimal HA117 siRNA. The pSOS-HUS vector method was successfully utilized as a rapid and effective screen for an optimal siRNA for a target gene. Among five pairs of DNA templates, siRNA transcribed from HAi5 gave the strongest interference with the novel gene HA117; a HAi5-carrying recombinant adenovirus (Ad-HAi5) was successfully constructed and evaluated, laying a foundation for the further study of HA117 gene function with RNAi technology
Where to Go Next for Recommender Systems? ID- vs. Modality-based Recommender Models Revisited
Recommendation models that utilize unique identities (IDs) to represent
distinct users and items have been state-of-the-art (SOTA) and dominated the
recommender systems (RS) literature for over a decade. Meanwhile, the
pre-trained modality encoders, such as BERT and ViT, have become increasingly
powerful in modeling the raw modality features of an item, such as text and
images. Given this, a natural question arises: can a purely modality-based
recommendation model (MoRec) outperforms or matches a pure ID-based model
(IDRec) by replacing the itemID embedding with a SOTA modality encoder? In
fact, this question was answered ten years ago when IDRec beats MoRec by a
strong margin in both recommendation accuracy and efficiency. We aim to revisit
this `old' question and systematically study MoRec from several aspects.
Specifically, we study several sub-questions: (i) which recommendation
paradigm, MoRec or IDRec, performs better in practical scenarios, especially in
the general setting and warm item scenarios where IDRec has a strong advantage?
does this hold for items with different modality features? (ii) can the latest
technical advances from other communities (i.e., natural language processing
and computer vision) translate into accuracy improvement for MoRec? (iii) how
to effectively utilize item modality representation, can we use it directly or
do we have to adjust it with new data? (iv) are there some key challenges for
MoRec to be solved in practical applications? To answer them, we conduct
rigorous experiments for item recommendations with two popular modalities,
i.e., text and vision. We provide the first empirical evidence that MoRec is
already comparable to its IDRec counterpart with an expensive end-to-end
training method, even for warm item recommendation. Our results potentially
imply that the dominance of IDRec in the RS field may be greatly challenged in
the future
A virus-like particle of the hepatitis B virus preS antigen elicits robust neutralizing antibodies and T cell responses in mice
The preS antigen of hepatitis B virus (HBV) corresponds to the N-terminal polypeptide in the large (L) antigen in addition to the small (S) antigen. The virus-like particle (VLP) of the S antigen is widely used as a vaccine to protect the population from HBV infection. The presence of the S antigen and its antibodies in patient blood has been used as markers to monitor hepatitis B. However, there is very limited knowledge about the preS antigen. We generated a preS VLP that is formed by a chimeric protein between preS and hemagglutinin (HA), and the matrix protein M1 of influenza virus. The HBV preS antigen is displayed on the surface of preS VLP. Asn112 and Ser98 of preS in VLP were found to be glycosylated and O-glycosylation of Ser98 has not been reported previously. The preS VLP shows a significantly higher immunogenicity than recombinant preS, eliciting robust anti-preS neutralizing antibodies. In addition, preS VLP is also capable of stimulating preS-specific CD8+ and CD4+ T cell responses in Balb/c mice and HBV transgenic mice. Furthermore, preS VLP immunization provided protection against hydrodynamic transfection of HBV DNA in mice. The data clearly suggest that this novel preS VLP could elicit robust immune responses to the HBV antigen, and can be potentially developed into prophylactic and therapeutic vaccines
NineRec: A Benchmark Dataset Suite for Evaluating Transferable Recommendation
Learning a recommender system model from an item's raw modality features
(such as image, text, audio, etc.), called MoRec, has attracted growing
interest recently. One key advantage of MoRec is that it can easily benefit
from advances in other fields, such as natural language processing (NLP) and
computer vision (CV). Moreover, it naturally supports transfer learning across
different systems through modality features, known as transferable recommender
systems, or TransRec.
However, so far, TransRec has made little progress, compared to
groundbreaking foundation models in the fields of NLP and CV. The lack of
large-scale, high-quality recommendation datasets poses a major obstacle. To
this end, we introduce NineRec, a TransRec dataset suite that includes a
large-scale source domain recommendation dataset and nine diverse target domain
recommendation datasets. Each item in NineRec is represented by a text
description and a high-resolution cover image. With NineRec, we can implement
TransRec models in an end-to-end training manner instead of using pre-extracted
invariant features. We conduct a benchmark study and empirical analysis of
TransRec using NineRec, and our findings provide several valuable insights. To
support further research, we make our code, datasets, benchmarks, and
leaderboards publicly available at https://github.com/westlake-repl/NineRec
To compare the efficacy of two kinds of Zhizhu pills in the treatment of functional dyspepsia of spleen-deficiency and qi-stagnation syndrome:a randomized group sequential comparative trial
<p>Abstract</p> <p>Background</p> <p>In Traditional Chinese Medicine (TCM) theory, functional dyspepsia (FD) can be divided into different syndromes according to different clinical symptoms and signs, and the most common one is spleen-deficiency and qi-stagnation syndrome that can be treated by Chinese traditional patent medicine ---- two kinds of Zhizhu pills, between which the primary difference in ingredients is that one contains immature orange fruit of Citrus aurantium L.(IFCA) and the other contains that of Citrus sinensis Osbeck (IFCS). The trial's objective was to compare the efficacy of two kinds of Zhizhu pills on symptom changes in patients with FD of spleen-deficiency and qi-stagnation syndrome.</p> <p>Methods</p> <p>A randomized, group sequential, double-blinded, multicenter trial was conducted in patients with FD of spleen-deficiency and qi-stagnation syndrome at 3 hospitals in Beijing between June 2003 and May 2005. Participants were randomly allocated into two groups (IFCA group and IFCS group) in a 1:1 ratio, and respectively took one of the two kinds of Zhizhu pills orally, 6 g each time, 3 times a day, for 4 weeks. Statistical analysis was performed with use of a group sequential method, the triangular test (TT).</p> <p>Results</p> <p>A total of 163 patients were randomized, and 3 patients were excluded from analysis because of early dropouts, leaving 160 patients (IFCA group: n = 82; IFCS group: n = 78) for statistical analysis. Three interim analyses were done after 62, 116, and 160 patients had completed their 4-week treatment, respectively. At the third interim analysis, the sample path crossed the upper boundary and the trial was stopped, the cure-markedly effective rates were 45% for IFCS group and 67% for IFCA group, respectively, the one-sided <it>p</it>-value was 0.0036, the median unbiased estimate of the odds ratio (OR) for the benefit of IFCA relative to IFCS was 2.91 with 95%CI: 1.40 to 6.06.</p> <p>No adverse events were observed in the two groups.</p> <p>Conclusions</p> <p>Zhizhu pills containing IFCA was superior to Zhizhu pills containing IFCS in the treatment of FD of spleen-deficiency and qi-stagnation syndrome. The application of group sequential analysis in clinical trials of TCM may offer some financial and ethical benefits.</p> <p>Trial Registration</p> <p>Chinese Clinical Trial Registry (ChiCTR): ChiCTR-TRC-00000485</p
Determination of Nitric Oxide-Derived Nitrite and Nitrate in Biological Samples by HPLC Coupled to Nitrite Oxidation
Multidifferential study of identified charged hadron distributions in -tagged jets in proton-proton collisions at 13 TeV
Jet fragmentation functions are measured for the first time in proton-proton
collisions for charged pions, kaons, and protons within jets recoiling against
a boson. The charged-hadron distributions are studied longitudinally and
transversely to the jet direction for jets with transverse momentum 20 GeV and in the pseudorapidity range . The
data sample was collected with the LHCb experiment at a center-of-mass energy
of 13 TeV, corresponding to an integrated luminosity of 1.64 fb. Triple
differential distributions as a function of the hadron longitudinal momentum
fraction, hadron transverse momentum, and jet transverse momentum are also
measured for the first time. This helps constrain transverse-momentum-dependent
fragmentation functions. Differences in the shapes and magnitudes of the
measured distributions for the different hadron species provide insights into
the hadronization process for jets predominantly initiated by light quarks.Comment: All figures and tables, along with machine-readable versions and any
supplementary material and additional information, are available at
https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-013.html (LHCb
public pages
Study of the decay
The decay is studied
in proton-proton collisions at a center-of-mass energy of TeV
using data corresponding to an integrated luminosity of 5
collected by the LHCb experiment. In the system, the
state observed at the BaBar and Belle experiments is
resolved into two narrower states, and ,
whose masses and widths are measured to be where the first uncertainties are statistical and the second
systematic. The results are consistent with a previous LHCb measurement using a
prompt sample. Evidence of a new
state is found with a local significance of , whose mass and width
are measured to be and , respectively. In addition, evidence of a new decay mode
is found with a significance of
. The relative branching fraction of with respect to the
decay is measured to be , where the first
uncertainty is statistical, the second systematic and the third originates from
the branching fractions of charm hadron decays.Comment: All figures and tables, along with any supplementary material and
additional information, are available at
https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-028.html (LHCb
public pages
Measurement of the ratios of branching fractions and
The ratios of branching fractions
and are measured, assuming isospin symmetry, using a
sample of proton-proton collision data corresponding to 3.0 fb of
integrated luminosity recorded by the LHCb experiment during 2011 and 2012. The
tau lepton is identified in the decay mode
. The measured values are
and
, where the first uncertainty is
statistical and the second is systematic. The correlation between these
measurements is . Results are consistent with the current average
of these quantities and are at a combined 1.9 standard deviations from the
predictions based on lepton flavor universality in the Standard Model.Comment: All figures and tables, along with any supplementary material and
additional information, are available at
https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-039.html (LHCb
public pages
Product Evaluation Prediction Model Based on Multi-Level Deep Feature Fusion
Traditional product evaluation research is to collect data through questionnaires or interviews to optimize product design, but the whole process takes a long time to deploy and cannot fully reflect the market situation. Aiming at this problem, we propose a product evaluation prediction model based on multi-level deep feature fusion of online reviews. It mines product satisfaction from the massive reviews published by users on e-commerce websites, and uses this model to analyze the relationship between design attributes and customer satisfaction, design products based on customer satisfaction. Our proposed model can be divided into the following four parts: First, the DSCNN (Depthwise Separable Convolutions) layer and pooling layer are used to combine extracting shallow features from the primordial data. Secondly, CBAM (Convolutional Block Attention Module) is used to realize the dimension separation of features, enhance the expressive ability of key features in the two dimensions of space and channel, and suppress the influence of redundant information. Thirdly, BiLSTM (Bidirectional Long Short-Term Memory) is used to overcome the complexity and nonlinearity of product evaluation prediction, output the predicted result through the fully connected layer. Finally, using the global optimization capability of the genetic algorithm, the hyperparameter optimization of the model constructed above is carried out. The final forecasting model consists of a series of decision rules that avoid model redundancy and achieve the best forecasting effect. It has been verified that the method proposed in this paper is better than the above-mentioned models in five evaluation indicators such as MSE, MAE, RMSE, MAPE and SMAPE, compared with Support Vector Regression (SVR), DSCNN, BiLSTM and DSCNN-BiLSTM. By predicting customer emotional satisfaction, it can provide accurate decision-making suggestions for enterprises to design new products
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