クウシン カ スル セカイ タイセン トマス ピンチョン ノ ジュウリョク ノ ニジ ト ギャッコウ

Cultural Formation Studies（2

Photoinduced Hydrogen Evolution Catalyzed by a Synthetic Diiron Dithiolate Complex Embedded within a Protein Matrix

The hydrogen-evolving diiron complex, (μ-S)₂Fe₂(CO)₆ with a tethered maleimide moiety was synthesized and covalently embedded within the cavity of a rigid β-barrel protein matrix by coupling a maleimide moiety to a cysteine residue within the β-barrel. The (μ-S)₂Fe₂(CO)₆ core within the cavity was characterized by UV–vis absorption and a characteristic CO vibration determined by IR measurements. The diiron complex embedded within the cavity retains the necessary catalytic activity (TON up to 130 for 6 h) to evolve H₂ via a photocatalytic cycle with a Ru photosensitizer in a solution of 100 mM ascorbate and 50 mM Tris/HCl at pH 4.0 and 25 °C

Solution x-ray-scattering study on the chaperonin groel from escherichia-coli

The molecular architecture of native GroEL has been studied by solution X-ray scattering. The radius of gyration for the native molecule was estimated to be 66.0 Å in 50 mM Tris-HCl, 100 mM KCl at pH 7.5 and 25°C. The maximum dimension was estimated to be 170 Å, based on the pair distance distribution function. A cylindrical structure or two heptameric rings was found to be the best for native GroEL among structures examined by using a multi-sphere model analysis in which the radius of constituent sphere was 6 Å. The results of the model analysis show that the radius of GroEL is 68.0 Å and the height is 150.7 Å. Unexpectedly, the central penetrating hole through GroEL was not confirmed in the best-fit structure