Sentidos diversos atribuídos pelos receptores a um comercial de TV

Dissertação de Mestrado apresentada como exigência parcial para obtenção do título de Mestre em Comunicação no Programa de Pós-graduação em Comunicação – Mestrado da Universidade Municipal de São Caetano do SulOs indivíduos expressam seus sentidos baseados na história vivida e nos valores construídos em sua trajetória pessoal. Esta base complexa e amplamente heterogênea abre um universo de dimensões e formas de agir e pensar a cada um que propõe sentidos diversos ao receber uma mensagem. Com o apoio dos conceitos de Cultura e Comunidade, essa pesquisa voltou-se para o receptor como sujeito importante no processo de comunicação e foram analisados os sentidos que ele tem ao assistir a um único comercial de TV. Partiu-se do perfil do público alvo do comercial do veículo Ford Fusion 2010 – Você fez por merecer. Teve como objetivo identificar os sentidos que diversos receptores podem atribuir ao assistir um comercial de televisão de veiculação nacional. Utilizou-se a metodologia da pesquisa qualitativa e registros de Narrativas Orais de História de Vida como suporte para dimensionar as vivências que foram relacionadas neste estudo, além dos conhecimentos e valores pessoais que constroem a sua identidade e imaginário sobre diversos assuntos. Destaca-se as referências mais importantes tratadas pelos próprios entrevistados a parir do próprio comercial assistido. Foram identificadas as diferenças dos sentidos entre os gêneros feminino e masculino, as relações de poder e status presentes no contexto social e profissional e as relações das diferenças encontradas entre a regionalidade vivida por cada dos entrevistados e o estereótipo construído pelos entrevistados quando identificado o contexto da cidade de São Paulo e dos paulistanos. Foi possível relacionar em capítulos os sentidos dos receptores que produziram temáticas semelhantes e visões e sentidos muitas vezes contrários entre eles. Visou-se tratar a importância dos estudos de recepção e cultura como forma de dimensionar um novo olhar para a publicidade e para o processo comunicacional

Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

A set of four duplex SYBR Green I PCR (SG-PCR) assay combined with DNA extraction using QIAamp DNA Stool Mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks. The causative pathogens were detected in almost all cases in 2 hours or less. The first run was for the detection of 8 main foodborne pathogens in 5 stool specimens within 2 hours and the second run was for the detection of other unusual suspect pathogens within a further 45 minutes. After 2 to 4 days, the causative agents were isolated and identified. The results proved that for comprehensive and rapid molecular diagnosis in foodborne outbreaks, Duplex SG-PCR assay is not only very useful, but is also economically viable for one-step differentiation of causative pathogens in fecal specimens obtained from symptomatic patients. This then allows for effective diagnosis and management of foodborne outbreaks

Characterization of a Thermostable 8-Oxoguanine DNA Glycosylase Specific for GO/N Mismatches from the Thermoacidophilic Archaeon Thermoplasma volcanium

The oxidation of guanine (G) to 7,8-dihydro-8-oxoguanine (GO) forms one of the major DNA lesions generated by reactive oxygen species (ROS). The GO can be corrected by GO DNA glycosylases (Ogg), enzymes involved in base excision repair (BER). Unrepaired GO induces mismatched base pairing with adenine (A); as a result, the mismatch causes a point mutation, from G paired with cytosine (C) to thymine (T) paired with adenine (A), during DNA replication. Here, we report the characterization of a putative Ogg from the thermoacidophilic archaeon Thermoplasma volcanium. The 204-amino acid sequence of the putative Ogg (TVG_RS00315) shares significant sequence homology with the DNA glycosylases of Methanocaldococcus jannaschii (MjaOgg) and Sulfolobus solfataricus (SsoOgg). The six histidine-tagged recombinant TVG_RS00315 protein gene was expressed in Escherichia coli and purified. The Ogg protein is thermostable, with optimal activity near a pH of 7.5 and a temperature of 60°C. The enzyme displays DNA glycosylase, and apurinic/apyrimidinic (AP) lyase activities on GO/N (where N is A, T, G, or C) mismatch; yet it cannot eliminate U from U/G or T from T/G, as mismatch glycosylase (MIG) can. These results indicate that TvoOgg-encoding TVG_RS00315 is a member of the Ogg2 family of T. volcanium

マンセイ シッカン デ セイシン ショウジョウ オ テイスル カンジャ エノ チイキ セイシンカ イリョウ モデル ジギョウ オヨビ ソノ ヒョウカ : セイシン カンゴ センモン カンゴフ ト リエゾン チーム ノ ヤクワリ

The purpose of this study was to show the effectiveness of psychiatric liaison consultation team for the people with chronic illness in the general hospital. Psychiatric liaison consultation team was constructed by certified nurse specialist (CNS), psychiatrist, clinical psychologist, nurse and social worker. Case manager was CNS and team intervened to the patients according to the standard of intervention by team. Intervention had been implemented by CNS mainly. Thirty one patients who consented to this study were intervened by CNS and team between July of 2007 and Feb.2008. They had chronic illness which was SLE, cancer and other physical illness without mental illness. BPRS, GAF, and LSP were written by CNS at the time of baseline before intervention and at the end time of intervention. Furthermore patients wrote CES-D and SF-36 by themselves at the baseline and the end point of intervention. Those questionnaires were returned by mail. CSQ was written by patients at the end of intervention. Thirty one patients had high depression score at the baseline and after intervention depressive state was improved. And they had lower SF-36 compared with that of the people who had chronic illness in another study. Furthermore they needed to be supported psychosocially by CNS to prevent worse their symptom. And they had psychosocial needs and those needs should be intervened by interdisciplinary team. In conclusion, the role of CNS and interdisciplinary team were discussed in order to meet the patients\u27 needs and to prevent worse their symptom in the general hospitals

Duplex Real-Time SYBR Green PCR Assays for Detection of 17 Species of Food- or Waterborne Pathogens in Stools

A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. The products formed were identified based on a melting point temperature (T(m)) curve analysis. The 17 species of food- or waterborne pathogens examined were enteroinvasive Escherichia coli, enteropathogenic E. coli, enterohemorrhagic E. coli, enterotoxigenic E. coli, enteroaggregative E. coli, Salmonella spp., Shigella spp., Yersinia enterocolitica, Yersinia pseudotuberculosis, Campylobacter jejuni, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Aeromonas spp., Staphylococcus aureus, Clostridium perfringens, and Bacillus cereus. No interference with the LC-PCR assay was observed when stool specimens were artificially inoculated with each bacterial species. The detection levels were approximately 10(5) food- or waterborne pathogenic bacteria per g of stool. The protocol for processing stool specimens for less than 10(4) food- or waterborne pathogenic bacteria per g of stool requires an overnight enrichment step to achieve adequate sensitivity. However, the rapid amplification and reliable detection of specific genes of greater than 10(5) food- or waterborne pathogenic bacteria per g in samples should facilitate the diagnosis and management of food- or waterborne outbreaks