Organic reactions mediated by electrochemically generated ArS+.

Low-temperature electrochemical oxidation of ArSSAr was carried out to generate a pool of "ArS(+)". Spectroscopic studies ((1)H NMR and CSI-MS) of the resulting solution revealed the accumulation of ArS(ArSSAr)(+). The resulting "ArS(+)" pool reacted with alkenes and alkynes to give diarylthio-substituted products. The "ArS(+)" pool rapidly reacted with thioacetals to give the corresponding alkoxycarbenium ion pools, which reacted with various carbon nucleophiles (indirect cation pool method). The reaction of the alkoxycarbenium ion pools with stilbene derivatives in the presence of ArSSAr gave thiochroman derivatives. In addition to such stoichiometric reactions, a catalytic amount of "ArS(+)" serves as an initiator and a chain carrier of some cationic chain reactions involving intramolecular carbon-carbon bond formation. In situ generation of "ArS(+)" by electrochemical oxidation of ArSSAr with a catalytic amount of electricity in the presence of a substrate is also effective for such cationic chain reactions

Role of low-ll component in deformed wave functions near the continuum threshold

The structure of deformed single-particle wave functions in the vicinity of zero energy limit is studied using a schematic model with a quadrupole deformed finite square-well potential. For this purpose, we expand the single-particle wave functions in multipoles and seek for the bound state and the Gamow resonance solutions. We find that, for the $K^{\pi}=0^{+}$ states, where $K$ is the $z$-component of the orbital angular momentum, the probability of each multipole components in the deformed wave function is connected between the negative energy and the positive energy regions asymptotically, although it has a discontinuity around the threshold. This implies that the $K^{\pi}=0^{+}$ resonant level exists physically unless the $l=0$ component is inherently large when extrapolated to the well bound region. The dependence of the multipole components on deformation is also discussed

Molecular Cloning of the Left-Terminal SmaI-D Fragment of Adenovirus Type 7 DNA

The left-terminal SmaI-D fragment (map position 0-13.5%) of adenovirus type 7 DNA has been cloned in the plasmid vector pBR322 by the homopolymeric tailings of poly (dG) and poly (dC). Two isolated clones were characterized by mapping the cleavage sites of restriction endonucleases and by sequencing the junction between viral insert and pBR322

Efficient Model Selection for Predictive Pattern Mining Model by Safe Pattern Pruning

Predictive pattern mining is an approach used to construct prediction models when the input is represented by structured data, such as sets, graphs, and sequences. The main idea behind predictive pattern mining is to build a prediction model by considering substructures, such as subsets, subgraphs, and subsequences (referred to as patterns), present in the structured data as features of the model. The primary challenge in predictive pattern mining lies in the exponential growth of the number of patterns with the complexity of the structured data. In this study, we propose the Safe Pattern Pruning (SPP) method to address the explosion of pattern numbers in predictive pattern mining. We also discuss how it can be effectively employed throughout the entire model building process in practical data analysis. To demonstrate the effectiveness of the proposed method, we conduct numerical experiments on regression and classification problems involving sets, graphs, and sequences

Requirement of Gαq/Gα11 Signaling in the Preservation of Mouse Intestinal Epithelial Homeostasis

Background & AimsProliferation, differentiation, and morphogenesis of the intestinal epithelium are tightly regulated by a number of molecular pathways. Coordinated action of intestine is achieved by gastrointestinal hormones, most of which exert these actions through G-protein–coupled receptors. We herein investigated the role of Gαq/11-mediated signaling in intestinal homeostasis.MethodsIntestinal tissues from control (Gnaqflox/floxGna11+/+), Int-Gq knock-out (KO) (VilCre+/-Gnaqflox/floxGna11+/+), G11 KO (Gnaqflox/floxGna11-/-), and Int-Gq/G11 double knock-out (DKO) (VilCre+/-Gnaqflox/floxGna11-/-) mice were examined by microscopy, transmission electron microscopy, and immunohistochemistry. The effect of Gαq/11-mediated signaling was studied in the cell lineage, proliferation, and apoptosis. Dextran sodium sulfate (DSS) colitis was induced to study the role of Gαq/11 in colon.ResultsPaneth cells were enlarged, increased in number, and mislocalized in Int-Gq/G11 DKO small intestine. Paneth cells also reacted with PAS and Muc2 antibody, indicating an intermediate character of Paneth and goblet cells. The nuclear β-catenin, T-cell factor 1, and Sox9 expression were reduced severely in the crypt base of Int-Gq/G11 DKO intestine. Proliferation was activated in the crypt base and apoptosis was enhanced along the crypt. Int-Gq/G11 DKO mice were susceptible to DSS colitis. Proliferation was inhibited in the crypt of unaffected and regenerative areas. Cystic crypts, periodic acid–Schiff–positive cells, and Muc2-positive cells were unusually observed in the ulcerative region.ConclusionsThe Gαq/11-mediated pathway plays a pivotal role in the preservation of intestinal homeostasis, especially in Paneth cell maturation and positioning. Wnt/β-catenin signaling was reduced significantly in the crypt base in Gαq/G11-deficient mice, resulting in the defective maturation of Paneth cells, induction of differentiation toward goblet cells, and susceptibility to DSS colitis

Restriction Endonuclease Cleavage Maps of the SmaI-D Fragment of Canine Adenovirus Type 1 DNA

The SmaI-E fragment (15 to 25 map unit position) of canine adenovirus type 1 DNA was cleaved into several specific fragments by restriction endonucleases, BglII, HapII, HinfI, HaeIII, Sau3A, TaqI, AluI, HhaI and Sau96I. These specific fragments were mapped on the canine adenovirus type 1 SmaI-E fragment by analyzing the partial digested products of single end-labeled DNAs