KIC 10417986: Spectroscopic confirmation of the nature of the binary system with a {\delta} Scuti component

KIC 10417986 is a short orbital period (0.0737 d) ellipsoidal variable star with a {\delta} Scuti and {\gamma} Doradus hybrid pulsations component discovered by Kepler. The ground-based spectroscopic observations were carried out in the winters of 2020 and 2021 to investigate the binary nature of this star. We derive the orbital parameters using the rvfit code with a result of K1 = 29.7 $\pm$ 1.5 km/s, {\gamma} = -18.7 $\pm$ 1.7 km/s, and confirm an orbital period of 0.84495 d instead of the result given by Kepler. The atmospheric parameters of the primary are determined by the synthetic spectra fitting technique with the estimated values of Teff = 7411 $\pm$ 187 K, log g = 4.2 $\pm$ 0.3 dex, [M/H] = 0.08 $\pm$ 0.09 dex and vsini = 52 $\pm$ 11 km/s. KIC 10417986 is a circular orbit binary system. From the single-lined nature and mass function of the star, the derived orbital inclination is 26 $\pm$ 6{\deg}, and the mass of the secondary is from 0.43 to 0.7 M_sun, which should be a late-K to early-M type star. Fourteen frequencies are extracted from Kepler light curves, of which six independent frequencies in the high-frequency region are identified as the p-mode pulsations of {\delta} Scuti star, and one independent frequency in the low-frequency region (f2 = 1.3033 c/d) is probably the rotational frequency due to the starspots rather than the ellipsoidal effect or g-mode of {\gamma} Doradus.Comment: 18 pages, 8 figure

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Study on the Influences of Empty Hole and In Situ Stress on Blasting-Induced Rock Damage

Blasting has been used extensively in deep underground excavation. In this paper, a numerical simulation LS-DYNA was carried out to investigate the influence of in situ stress and empty hole on rock fracture development. Based on the simulation process and existing laboratory data, the micromechanical properties of the Riedel-Hiermaier-Thoma (RHT) model were calibrated. By then, this model was used to study the effects of in situ stress and empty hole on the degree of damage and fracture development in the rock. Simulation results revealed that the difference between horizontal and vertical principal stresses can influence the degree of damage as well as the fracture initiation and development, which in turn leads to the fracture development towards a high-stress concentration area. Numerical simulation also showed that the total area of fracture development gradually decreases with the increasing in situ stress magnitudes. Under the same stress conditions, empty hole can facilitate the fracture development at 90°, while preventing the subsequent fracture development at 60° and 120°. This would result in further fracture propagation at 90°, such that the control of fracture direction can be achievable

Down-regulation of EGFL8 regulates migration, invasion and apoptosis of hepatocellular carcinoma through activating Notch signaling pathway

Abstract Background Our previous studies have reported the down-regulation of EGFL8 correlates to the development and prognosis of colorectal and gastric cancer. The present study is carried out to explore the expression pattern and role of EGFL8 in hepatocellular carcinoma (HCC). Methods and materials EGFL8 expression in 102 cases of HCC tissues matched with adjacent non-tumorous liver tissues, a normal liver cell line and three liver cancer cell lines with different metastatic capacity was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot. Moreover, the clinicopathological features and prognosis of HCC patients were correlated with expression of EGFL8. Subsequently, the gain-and loss-of-function experiments were carried out to investigate the biological function of EGFL8 in HCC. We also used N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-(S)- phenylglycine t-butyl ester (DAPT), an inhibitor for Notch signaling pathway, in these experiments to verify the involvement of Notch signaling pathway in the effects of EGFL8. Additionally, a mouse model was established to investigate the effect of EGFL8 on metastasis of HCC cells. The expression of Notch signaling pathway in HCC cells and xenograft mouse tumors were detected by Western blot and immunohistochemistory. Results The expression of EGFL8 was significantly decreased in HCC tissues and cell lines and EGFL8 down-regulation correlated to multiple nodules, vein invasion, high TNM stage and poor prognosis of HCC. Interestingly, the expression levels of EGFL8 in three liver cancer cell lines were negatively associated with their metastatic capacity. In vitro and in vivo experiments indicated that EGFL8 obviously suppressed metastasis and invasion of HCC cells but slightly promoted apoptosis. Meanwhile, the expression of Notch signaling pathway was obviously suppressed in EGFL8 overexpressed HCCLM3 cells and xenograft mouse tumors generated from these cells but markedly elevated in EGFL8 depleted Hep3B cells. Furthermore, the up-regulated expression of Notch signaling pathway and effects induced by EGFL8 knockdown in Hep3B cells could be counteracted by DAPT treatment. Conclusion The down-regulation of EGFL8 was correlated to progression and poor prognosis of HCC and regulates HCC cell migration, invasion and apoptosis through activating the Notch signaling pathway, suggesting EGFL8 as a novel therapeutic target and a potential prognostic marker for HCC

GmBZL3 acts as a major BR signaling regulator through crosstalk with multiple pathways in Glycine max

Abstract Background Brassinosteroids (BRs) play a crucial role in plant vegetative growth and reproductive development. The transcription factors BZR1 and BES1/BZR2 are well characterized as downstream regulators of the BR signaling pathway in Arabidopsis and rice. Soybean contains four BZR1-like proteins (GmBZLs), and it was reported that GmBZL2 plays a conserved role in BR signaling regulation. However, the roles of other GmBZLs have not been thoroughly studied, and the targets of GmBZLs in soybean remain unclear. Results In this study, we first characterized GmBZL3 in soybean from gene expression patterns, conserved domains in coding sequences, and genomic replication times of four GmBZL orthologous. The results indicated that GmBZL3 might play conserved roles during soybean development. The overexpression of GmBZL3 P219L in the Arabidopsis BR-insensitive mutant bri1–5 partially rescued the phenotypic defects including BR-insensitivity, which provides further evidence that GmBZL3 functions are conserved between soybean and the homologous Arabidopsis genes. In addition, the identification of the GmBZL3 target genes through ChIP-seq technology revealed that BR has broad roles in soybean and regulates multiple pathways, including other hormone signaling, disease-related, and immunity response pathways. Moreover, the BR-regulated GmBZL3 target genes were further identified, and the results demonstrate that GmBZL3 is a major transcription factor responsible for BR-regulated gene expression and soybean growth. A comparison of GmBZL3 and AtBZR1/BES1 targets demonstrated that GmBZL3 might play conserved as well as specific roles in the soybean BR signaling network. Finally, the identification of two natural soybean varieties of the GmBZL3 mutantion by SNP analysis could facilitate the understanding of gene function during soybean development in the future. Conclusions We illustrate here that GmBZL3 orchestrates a genome-wide transcriptional response that underlies BR-mediated soybean early vegetative growth, and our results support that BRs play crucial regulatory roles in soybean morphology and gene expression levels

Resource enrichment combined with biomass removal maintains plant diversity and community stability in a long-term grazed grassland

Aims Long-term heavy grazing reduces plant diversity and ecosystem function by intensifying nitrogen (N) and water limitation. In contrast, the absence of biomass removal can cause species loss by elevating light competition and weakening community stability, which is exacerbated by N and water enrichment. Hence, how to maintain species diversity and community stability is still a huge challenge for sustainable management of worldwide grasslands. Methods We conducted a 4-year manipulated experiment in six long-term grazing blocks to explore combination of resource additions and biomass removal (increased water, N and light availability) on species richness and community stability in semiarid grasslands of Inner Mongolia, China. Important Findings In all blocks treated with the combination of resource additions and biomass removal, primary productivity increased and species richness arid community stability were maintained over 4 years of experiment. At both species arid plant functional group (PFG) levels, the aboveground biomass of treated plants remained temporally stable in treatments with the combination of N and/or water addition and biomass removal. The maintenance of species richness was primarily caused by the biomass removal, which could increase the amount of light exposure for grasses under resource enrichment. Both species asynchrony and stability of PFGs contributed to the high temporal stability observed in these communities. Our results indicate that management practices of combined resource enrichment with biomass removal, such as grazing or mowing, could not only enhance primary productivity but also maintain plant species diversity, species asynchrony and community stability. Furthermore, as overgrazing-induced degradation and resource enrichment-induced biodiversity loss continue to be major problems worldwide, our findings have important implications for adaptive management in semiarid grasslands and beyond

Compressive near-field millimeter wave imaging algorithm based on gini index and total variation mixed regularization

A compressive near-field millimeter wave (MMW) imaging algorithm is proposed. From the compressed sensing (CS) theory, the compressive near-field MMW imaging process can be considered to reconstruct an image from the under-sampled sparse data. The Gini index (GI) has been founded that it is the only sparsity measure that has all sparsity attributes that are called Robin Hood, Scaling, Rising Tide, Cloning, Bill Gates, and Babies. By combining the total variation (TV) operator, the GI-TV mixed regularization introduced compressive near-field MMW imaging model is proposed. In addition, the corresponding algorithm based on a primal-dual framework is also proposed. Experimental results demonstrate that the proposed GI-TV mixed regularization algorithm has superior convergence and stability performance compared with the widely used l1-TV mixed regularization algorithm

Diterpenoids from Saliva plebeia R. Br. and Their Antioxidant and Anti-Inflammatory Activities

A new skeleton of diterpenoid, 1,2,3,4,4α,9,10,10α-octahydro-(4α-hydroxyymethyl) -1,1-dimethyl-9-(1-methylethyl)-(2S,3S,4αR,9R,10αS)-2,3,5,7-phenanthrenetertrol, named plebeianiol A (1), along with four known diterpenoids (2–5), were isolated from Salvia plebeia R. Br. Their structures were determined on the basis of spectral analysis. In the bioactivity tests, compounds 1, 2 and 5 showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities with IC50 values of 20.0–29.6 µM. In addition, these three compounds had significant inhibitory effects on reactive oxygen species (ROS) production in lipopolysaccharide (LPS)-induced macrophages. Compounds 1–3 inhibited nitric oxide (NO) production in LPS-induced macrophages with IC50 values of 18.0–23.6 µM. These results showed that compounds 1, 2 had significant antioxidant and anti-inflammatory activities and might provide basis for the treatment of diseases associated with oxidative lesions and inflammation