Fatty acid composition of Nile tilapia (Oreochromis niloticus) and Red hybrid tilapia (Oreochromis mossambicus X Oreochromis niloticus) reared in intensive and extensive systems

Fish and marine mammals are among the richest sources of long–chain n-3 and n-6 polyunsaturated fatty acids (PUFA) in nature. As farmed fish becomes a major contributor to world fish supplies, hence it is important to maintain the high lipid nutritional quality of the product and to continue to provide large amounts of the health-promoting n-3 and n-6 PUFA to the consumers. Therefore, a study was conducted to examine the nutritional content, mainly fatty acid composition, of Nile (Oreochromis niloticus and Red hybrid tilapia (Oreochromis mossambicus x Oreochromis niloticus), derived from intensive and extensive culture systems. Twenty-two samples of Nile tilapia and 16 samples of Red tilapia cultured intensively and 10 samples of extensively cultured Nile tilapia were used in this study. All samples were subjected to total fatty acids extraction and their fatty acid compositions were determined using gas liquid chromatography. Results showed no significant differences in the concentration of saturated fatty acids (SFA) between intensively and extensively cultured Nile tilapia. For monounsaturated fatty acids (MUFA), the concentration was higher (p<0.05) in the intensively cultured Nile tilapia compared to the extensively cultured Nile tilapia. For n-3 PUFA, the concentration was higher (p<0.05) in Nile tilapia from the extensive system compared to Nile tilapia from the intensive system. But for n-6 PUFA, the intensively cultured Nile tilapia had a higher (p<0.05) concentration compared to the extensively cultured Nile tilapia. For n-6:n-3 PUFA ratio the intensively cultured Nile tilapia had a higher ratio (p<0.05) than the extensively cultured Nile tilapia. The different fatty acid composition of Nile tilapia cultured in different systems could be due to the different types of feed consumed by the fish. Future research could be directed at increasing the PUFA level in tilapia through the manipulation of the PUFA level in the fish diet

Na+-leak channel, non-selective (NALCN) regulates myometrial excitability and facilitates successful parturition

Background/Aims: Uterine contractility is controlled by electrical signals generated by myometrial smooth muscle cells. Because aberrant electrical signaling may cause inefficient uterine contractions and poor reproductive outcomes, there is great interest in defining the ion channels that regulate uterine excitability. In human myometrium, the Na+ leak channel, non-selective (NALCN) contributes to a gadolinium-sensitive, Na+-dependent leak current. The aim of this study was to determine the role of NALCN in regulating uterine excitability and examine its involvement in parturition. Methods: Wildtype C57BL/6J mice underwent timed-mating and NALCN uterine expression was measured at several time points across pregnancy including pregnancy days 7, 10, 14, 18 and 19. Sharp electrode current clamp was used to measure uterine excitability at these same time points. To determine NALCN’s contribution to myometrial excitability and pregnancy outcomes, we created smooth-muscle-specific NALCN knockout mice by crossing NALCNfx/fx mice with myosin heavy chain Cre (MHCCreeGFP) mice. Parturition outcomes were assessed by observation via surveillance video recording cre control, flox control, smNALCN+/-, and smNALCN-/- mice. Myometrial excitability was compared between pregnancy day 19 flox controls and smNALCN-/- mice. Results: We found that in the mouse uterus, NALCN protein levels were high early in pregnancy, decreased in mid and late pregnancy, and then increased in labor and postpartum. Sharp electrode current clamp recordings of mouse longitudinal myometrial samples from pregnancy days 7, 10, 14, 18, and 19 revealed day-dependent increases in burst duration and interval and decreases in spike density. NALCN smooth muscle knockout mice had reduced myometrial excitability exemplified by shortened action potential bursts, and an increased rate of abnormal labor, including prolonged and dysfunctional labor. Conclusions: Together, our findings demonstrate that the Na+ conducting channel NALCN contributes to the myometrial action potential waveform and is important for successful labor outcomes

Financial well-being among Malaysian manufacturing employees

Employees and financial well-being are two aspects that are closely related to each other, and have been deeply studied by researchers. Not only can financial well-being directly affect an individual, but it can also indirectly affect his/her organization as well as employer. Any level of financial employees’ well-being, either low or high, will change their job performance. Thus, the purpose of this study is to determine the level of financial well-being among manufacturing employees in Batu Pahat, as well as to test the relationships between determinants and financial well-being among manufacturing employees in Batu Pahat. In this study, seven research hypotheses were developed to examine seven determinants, including age, income, gender, education, current job position, income, and marital status which influence employees‘ financial well-being. In this study, 220 employees at the production level were selected randomly from a manufacturing company in Batu Pahat, Johor, Malaysia. Then, a questionnaire was distributed to the employees. The data obtained were analyzed quantitatively using SPSS version 22.0. The results of this study revealed that the level of financial well-being was moderate and all of the determinants were positively related to financial well-being among the manufacturing employees. This quantitative study is important to the manufacturing industry in Malaysia in order to gain insight on the correlation between financial well-being and its determinants

Magnetically Actuated Cell Stretching Platform to Induce Phenotypic Changes in Metastatic Cells

Although metastasis is responsible for about 90% of cancer deaths, only few in vitro models can be used to evaluate dynamic behaviors of metastatic cancer cells. Many studies have shown that mechanical stimuli can trigger various cellular responses such as gene and protein expression, which could lead to changes in cellular phenotype. Similarly, metastasized breast cancer cells in the lung tissue are constantly stretched by cyclic mechanical stress due to breathing, which alters cellular morphology and proliferation state. Such transitions can make the secondary tumors resistant to the chemotherapy used to effectively treat the primary tumors. In this work, we developed an in vitro tumor microenvironment that simulates in vivo respiration to investigate the mechanism of the phenotypic changes of metastatic breast cancer cells due to mechanical stimulation. We designed and fabricated magnetic microactuators using maskless photolithography technique to stretch tumor cells. Next, we coated fibronectin fibrils over the gaps of microactuators to mimic natural ECM environment and seeded tumor cells on the fibronectin mesh to generate a tumor microenvironment. As a result, the amount of strain that our microdevice could apply on the fibronectin mesh corresponded to the amount of strain experienced during normal respiration. In conclusion, the magnetically actuated in vitro cell stretching platform can provide precise strain control over a large actuation range to mimic mechanical stimulation in the lung. In the future, we will evaluate potential changes in metastatic cell phenotype and provide additional insights on the mechanism of secondary tumor drug resistance

User Interface Engineering (KSU)

This Grants Collection for User Interface Engineering was created under a Round Twelve ALG Textbook Transformation Grant. Affordable Learning Georgia Grants Collections are intended to provide faculty with the frameworks to quickly implement or revise the same materials as a Textbook Transformation Grants team, along with the aims and lessons learned from project teams during the implementation process. Documents are in .pdf format, with a separate .docx (Word) version available for download. Each collection contains the following materials: Linked Syllabus Initial Proposal Final Reporthttps://oer.galileo.usg.edu/compsci-collections/1035/thumbnail.jp

Phylogeny of Amphidinium (Dinophyceae) from Guam and Okinawa, with descriptions of A. pagoense sp. nov. and A. uduigamense sp. nov.

Marine benthic dinoflagellates within the genus Amphidinium were isolated from Guam and Okinawa. Isolated strains were identified to species-level using phylogenetic analyses of 28S rRNA and ITS-5.8S rRNA genes as well as microscopy. Of the six isolated strains, two were new species: A. pagoense sp. nov. and A. uduigamense sp. nov. Other isolates included strains of A. massartii and A. operculatum from Guam, and two strains of A. operculatum from Okinawa. Both new species were described using light and electron microscopy (SEM and TEM). The combination of characteristics that make A. pagoense sp. nov. unique includes a pair of centrally-located pyrenoids, variable cell shape, absence of scales and a long, curved ventral ridge. For A. uduigamense sp. nov., a combination of several morphological features distinguishes it from other species. These include a constriction near the anterior of the hypocone, two centrally located pyrenoids, a longitudinal flagellum inserted in the posterior one-third of the cell, cell size, cell division in the motile stage and the absence of scales. Toxicity was confirmed in these two novel species by testing methanol extracts in an Artemia bioassay. Previously unrecorded ITS rRNA gene sequences from A. operculatum were also sequenced from both locations. Species identified and newly described in this study expand the taxonomic knowledge of Amphidinium in the Pacific.journal articl

Mobile Software Development (KSU)

This Grants Collection for Mobile Software Development was created under a Round Twelve ALG Textbook Transformation Grant. Affordable Learning Georgia Grants Collections are intended to provide faculty with the frameworks to quickly implement or revise the same materials as a Textbook Transformation Grants team, along with the aims and lessons learned from project teams during the implementation process. Documents are in .pdf format, with a separate .docx (Word) version available for download. Each collection contains the following materials: Linked Syllabus Initial Proposal Final Reporthttps://oer.galileo.usg.edu/compsci-collections/1036/thumbnail.jp

Specialized adaptation of a lactic acid bacterium to the milk environment: the comparative genomics of Streptococcus thermophilus LMD-9

<p>Abstract</p> <p>Background</p> <p><it>Streptococcus thermophilus</it> represents the only species among the streptococci that has “Generally Regarded As Safe” status and that plays an economically important role in the fermentation of yogurt and cheeses. We conducted comparative genome analysis of <it>S. thermophilus</it> LMD-9 to identify unique gene features as well as features that contribute to its adaptation to the dairy environment. In addition, we investigated the transcriptome response of LMD-9 during growth in milk in the presence of <it>Lactobacillus delbrueckii</it> ssp. <it>bulgaricus</it>, a companion culture in yogurt fermentation, and during lytic bacteriophage infection.</p> <p>Results</p> <p>The <it>S. thermophilus</it> LMD-9 genome is comprised of a 1.8 Mbp circular chromosome (39.1% GC; 1,834 predicted open reading frames) and two small cryptic plasmids. Genome comparison with the previously sequenced LMG 18311 and CNRZ1066 strains revealed 114 kb of LMD-9 specific chromosomal region, including genes that encode for histidine biosynthetic pathway, a cell surface proteinase, various host defense mechanisms and a phage remnant. Interestingly, also unique to LMD-9 are genes encoding for a putative mucus-binding protein, a peptide transporter, and exopolysaccharide biosynthetic proteins that have close orthologs in human intestinal microorganisms. LMD-9 harbors a large number of pseudogenes (13% of ORFeome), indicating that like LMG 18311 and CNRZ1066, LMD-9 has also undergone major reductive evolution, with the loss of carbohydrate metabolic genes and virulence genes found in their streptococcal counterparts. Functional genome distribution analysis of ORFeomes among streptococci showed that all three <it>S. thermophilus</it> strains formed a distinct functional cluster, further establishing their specialized adaptation to the nutrient-rich milk niche. An upregulation of CRISPR1 expression in LMD-9 during lytic bacteriophage DT1 infection suggests its protective role against phage invasion. When co-cultured with <it>L. bulgaricus</it>, LMD-9 overexpressed genes involved in amino acid transport and metabolism as well as DNA replication.</p> <p>Conclusions</p> <p>The genome of <it>S. thermophilus</it> LMD-9 is shaped by its domestication in the dairy environment, with gene features that conferred rapid growth in milk, stress response mechanisms and host defense systems that are relevant to its industrial applications. The presence of a unique exopolysaccharide gene cluster and cell surface protein orthologs commonly associated with probiotic functionality revealed potential probiotic applications of LMD-9.</p