31 research outputs found
B-cell memory in malaria : Myths and realities
B-cell and antibody responses to Plasmodium spp., the parasite that causes malaria, are critical for control of parasitemia and associated immunopathology. Antibodies also provide protection to reinfection. Long-lasting B-cell memory has been shown to occur in response to Plasmodium spp. in experimental model infections, and in human malaria. However, there are reports that antibody responses to several malaria antigens in young children living with malaria are not similarly long-lived, suggesting a dysfunction in the maintenance of circulating antibodies. Some studies attribute this to the expansion of atypical memory B cells (AMB), which express multiple inhibitory receptors and activation markers, and are hyporesponsive to B-cell receptor (BCR) restimulation in vitro. AMB are also expanded in other chronic infections such as tuberculosis, hepatitis B and C, and HIV, as well as in autoimmunity and old age, highlighting the importance of understanding their role in immunity. Whether AMB are dysfunctional remains controversial, as there are also studies in other infections showing that AMB can produce isotype-switched antibodies and in mouse can contribute to protection against infection. In light of these controversies, we review the most recent literature on either side of the debate and challenge some of the currently held views regarding B-cell responses to Plasmodium infections
A longitudinal cohort study of malaria exposure and changing serostatus in a malaria endemic area of rural Tanzania.
BACKGROUND: Measurements of anti-malarial antibodies are increasingly used as a proxy of transmission intensity. Most serological surveys are based on the use of cross-sectional data that, when age-stratified, approximates historical patterns of transmission within a population. Comparatively few studies leverage longitudinal data to explicitly relate individual infection events with subsequent antibody responses. METHODS: The occurrence of seroconversion and seroreversion events for two Plasmodium falciparum asexual stage antigens (MSP-1 and AMA-1) was examined using three annual measurements of 691 individuals from a cohort of individuals in a malaria-endemic area of rural east-central Tanzania. Mixed-effect logistic regression models were employed to determine factors associated with changes in serostatus over time. RESULTS: While the expected population-level relationship between seroprevalence and disease incidence was observed, on an individual level the relationship between individual infections and the antibody response was complex. MSP-1 antibody responses were more dynamic in response to the occurrence and resolution of infection events than AMA-1, while the latter was more correlated with consecutive infections. The MSP-1 antibody response to an observed infection seemed to decay faster over time than the corresponding AMA-1 response. Surprisingly, there was no evidence of an age effect on the occurrence of a conversion or reversion event. CONCLUSIONS: While the population-level results concur with previously published sero-epidemiological surveys, the individual-level results highlight the more complex relationship between detected infections and antibody dynamics than can be analysed using cross-sectional data. The longitudinal analysis of serological data may provide a powerful tool for teasing apart the complex relationship between infection events and the corresponding immune response, thereby improving the ability to rapidly assess the success or failure of malaria control programmes
Antibody responses to merozoite antigens after natural Plasmodium falciparum infection: kinetics and longevity in absence of re-exposure
Background Antibodies against merozoite antigens are key components of malaria immunity. The naturally acquired antibody response to these antigens is generally considered short-lived; however, the underlying mechanisms remain unclear. Prospective studies of travellers with different levels of prior exposure, returning to malaria-free countries with Plasmodium infection, offer a unique opportunity to investigate the kinetics and composition of the antibody response after natural infection. Methods Adults diagnosed with P. falciparum malaria in Stockholm, Sweden (20 likely malaria naïve and 41 with repeated previous exposure during residency in sub-Saharan Africa) were sampled at diagnosis and 10 days and 1, 3, 6, and 12 months after treatment. Total and subclass-specific IgG responses to P. falciparum merozoite antigens (AMA-1, MSP-119, MSP-2, MSP-3, and RH5) and tetanus toxoid were measured by multiplex bead-based immunoassays and ELISA. Mathematical modelling was used to estimate the exposure-dependent longevity of antibodies and antibody-secreting cells (ASCs). Results A majority of individuals mounted detectable antibody responses towards P. falciparum merozoite antigens at diagnosis; however, the magnitude and breadth were greater in individuals with prior exposure. In both exposure groups, antibody levels increased rapidly for 2 weeks and decayed thereafter. Previously exposed individuals maintained two- to ninefold greater antibody levels throughout the 1-year follow-up. The half-lives of malaria-specific long-lived ASCs, responsible for maintaining circulating antibodies, ranged from 1.8 to 3.7 years for merozoite antigens and were considerably short compared to tetanus-specific ASCs. Primary infected individuals did acquire a long-lived component of the antibody response; however, the total proportion of long-lived ASCs generated in response to infection was estimated not to exceed 10%. In contrast, previously exposed individuals maintained substantially larger numbers of long-lived ASCs (10–56% of total ASCs). Conclusion The short-lived nature of the naturally acquired antibody response, to all tested merozoite antigens, following primary malaria infection can be attributed to a combination of a poor acquisition and short half-life of long-lived ASCs. Greater longevity is acquired with repeated infections and can be explained by the maintenance of larger numbers of long-lived ASCs. These insights advance our understanding of naturally acquired malaria immunity and will guide strategies for further development of both vaccines and serological tools to monitor exposure.</p
The allergens of dog II. Identification and partial purification of a major dander allergen
Modeling long-term malaria transmission changes in a Tanzanian village using cross-sectional data on age specific prevalence and levels of antibodies.
Robust estimates of Plasmodium falciparum transmission intensity are imperative for planning, implementation and evaluation of malaria control interventions. Seroconversion rates (SCR) to asexual blood-stage antigens can provide estimates of transmission intensity that correlate with entomological inoculation rates. We study past transmission trends in a rural village and evaluate new models to improve SCR based methods by analysis of antibody levels from multiple cross-sectional serological surveys. The study was conducted in Nyamisati, Rufiji, Tanzania, where parasite prevalence decreased from 65 to 18% from 1999 and 2010. A single intervention with ITNs was performed in 1999. IgG levels to recombinant P. falciparum antigens (MSP-119, MSP-2, MSP-3, AMA-1) and An. gambiae salivary protein gSG6 were measured in children (1-16y) sampled in cross-sectional surveys in 1999 and 2010. SCR and rates of antibody decay were estimated by fitting mathematical models to data from the two cross-sections, assuming three profiles of exposure: (i) stable; (ii) stepwise decrease; or (iii) continuous decrease. Results suggest an average 66% decrease in malaria transmission intensity and an 89% reduction in Anopheles exposure. Transmission trends were best described by a stepwise decrease model with a reduction predicted to occur shortly after distribution of ITNs. The new models provide estimates of the duration of antibody responses under this transmission decline. MSP-119 seropositive individuals were estimated to convert to seronegative with a half-life of 12 (95% CI 7-20) years due to antibody decline with a half-life of 3 (95% CI 2-6) years. The reduction in transmission may in part be attributed to reduced anopheles exposure following the introduction of ITNs, but is not likely to be explained by ITNs alone. Despite reduced parasite prevalence many children remained seropositive to blood-stage antigens. The new sensitive models using antibody levels enabled detection of reduced exposure among seropositive children and provided estimates of both antibody and transmission dynamics
Memory B-cell responses against merozoite antigens after acute Plasmodium falciparum malaria, assessed over one year using a novel multiplexed fluorospot assay
Memory B cells (MBCs) are believed to be important for the maintenance of immunity to malaria, and these cells need to be explored in the context of different parasite antigens and their breadth and kinetics after natural infections. However, frequencies of antigen-specific MBCs are low in peripheral blood, limiting the number of antigens that can be studied, especially when small blood volumes are available. Here, we developed a multiplexed reversed B-cell FluoroSpot assay capable of simultaneously detecting MBCs specific for the four Plasmodium falciparum blood-stage antigens, MSP-119, MSP-2, MSP-3 and AMA-1. We used the assay to study the kinetics of the MBC response after an acute episode of malaria and up to one year following treatment in travelers returning to Sweden from sub-Saharan Africa. We show that the FluoroSpot assay can detect MBCs to all four merozoite antigens in the same well, and that the breadth and kinetics varied between individuals. We further found that individuals experiencing a primary infection could mount and maintain parasite-specific MBCs to a similar extent as previously exposed adults, already after a single infection. We conclude that the multiplexed B-cell FluoroSpot is a powerful tool for assessing antigen-specific MBC responses to several antigens simultaneously, and that the kinetics of MBC responses against merozoite surface antigens differ over the course of one year. These findings contribute to the understanding of acquisition and maintenance of immune responses to malaria
Evaluating changing malaria transmission in a rural village, in coastal Tanzania, by assessment of IgG antibodies against Plasmodium falciparum and Anopheles gambiae antigens
The changing epidemiology of malaria in Sub-Saharan Africa over the last decade needs further understanding. A longitudinal project on the epidemiology of malaria was set up in the Nyamisati village, Rufiji District, Tanzania in 1985. Cross-sectional surveys have revealed a gradual decrease in parasite prevalence over the last 25 years, from >90% to 13% (by PCR), despite the absence of large scale control interventions. Although PCR provides good detection of low level parasitemia, assessment of parasite prevalence alone may not fully reflect the level of exposure in a population. In order to further understand the long-term dynamics of malaria transmission in this setting, we combined an assessment of parasite prevalence with analyses of IgG antibodies against antigens of the P. falciparum parasite and a salivary antigen of the Anopheles vector. The study included children 1–16 years old participating in cross-sectional surveys in Nyamisati village in 1995, 1999 and 2010. Parasite prevalence was established by PCR. Detection and quantification of IgG antibodies against P. falciparum crude schizont extract (PfSE) and Anopheles gambiae salivary gland protein 6 (gSG6) were performed with enzyme-linked immunosorbent assay (ELISA). Levels of IgG antibodies against merozoite surface protein 1–19 (MSP-119) and merozoite surface protein 2 (MSP-2) were assessed using a multiplexed bead-based immunoassay. Parasite prevalence and the prevalence and levels of IgG antibodies against PfSE, MSP-119 and MSP-2 decreased between 1999 and 2010, with the most marked reduction among the youngest children (1–4 years of age). The prevalence and levels of anti-gSG6 IgG antibodies showed a similar pattern. These findings suggest a substantially decreased intensity of malaria transmission in the Nyamisati area over the past 10 years, together with an evident decrease in the population level of exposure to the malaria vector
Understanding changing malaria transmission in a rural village, in coastal Tanzania, by assessment of IgG antibodies against Plasmodium falciparum and Anopheles gambiae antigens.
Background: The changing epidemiology of malaria in Sub-Saharan Africa over the last decade needs further understanding. A longitudinal project on the epidemiology of malaria was set up in the Nyamisati village, Rufiji District, Tanzania in 1985. Cross-sectional surveys have revealed a gradual decrease in parasite prevalence over the last 25 years, from >90% to 13% (by PCR), despite the absence of large scale control interventions. Although PCR improves the detection of low-level parasitemia, assessment of parasite prevalence alone may not fully reflect the level of exposure in a population. In order to further understand the long-term dynamics of malaria transmission in this setting, we combined an assessment of parasite prevalence with analyses of IgG antibodies against antigens of the P. falciparum parasite and a salivary antigen of the Anopheles vector.
Methodology: The study included children 1-16 years old participating in cross-sectional surveys in Nyamisati village in 1995, 1999 and 2010. Parasite prevalence was established by microscopy and PCR. Detection and quantification of IgG antibodies against P. falciparum whole schizont extract (PfSE) and Anopheles gambiae salivary gland protein 6 (gSG6) were performed with enzyme-linked immunosorbent assay (ELISA). Levels of IgG antibodies against merozoite surface protein 1-19 (MSP-119) and merozoite surface protein 2 (MSP-2) were assessed using a multiplexed bead-based immunoassay.
Results and conclusions: The prevalence of anti-PfSE IgG as well as the levels of IgG antibodies against MSP-119 and MSP-2 decreased between 1995 and 2010, with the most marked reduction among the youngest children (1-4 years of age). The levels of anti-gSG6-IgG were, however, relatively stable over time. These findings suggest a substantially decreased intensity of malaria transmission in the Nyamisati area over the past 15 years, without an equally evident decrease in the population level of exposure to the malaria vector
Quantitation of the major allergen of several Parietaria pollens by an anti-Par 1 monoclonal antibody-based ELISA. Analysis of crossreactivity among purified Par j 1, Par o 1 and Par m 1 allergens
Multiplicity of asymptomatic Plasmodium falciparum infections and risk of clinical malaria : a systematic review and pooled analysis of individual participant data
Background. The malaria parasite Plasmodium falciparum holds an extensive genetic polymorphism. In this pooled analysis, we investigate how the multiplicity in asymptomatic P. falciparum infections-that is, the number of coinfecting clones-affects the subsequent risk of clinical malaria in populations living under different levels of transmission. Methods. A systematic search of the literature was performed to identify studies in which P. falciparum infections were genotyped in asymptomatic individuals who were followed up prospectively regarding the incidence of clinical malaria. Individual participant data were pooled from 15 studies (n = 3736 individuals). Results. Multiclonal asymptomatic infections were associated with a somewhat increased subsequent risk of clinical malaria in the youngest children, followed by an initial declining risk with age irrespective of transmission intensity. At approximately 5 years of age, the risk continued the gradual decline with age in high-transmission settings. However, in older children in moderate-, low-, and seasonal-transmission settings, multiclonal infections were either not significantly associated with the risk of subsequent febrile malaria or were associated with an increased risk. Conclusions. The number of clones in asymptomatic P. falciparum infections is associated with different risks of subsequent clinical malaria depending on age and transmission intensity