11 research outputs found

    COVID-19 and the Chinese Christian Community in Britain: Changing Patterns of Belonging and Division

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    This article draws on interview data with Chinese Christian leaders to explore how the coronavirus pandemic is affecting the Chinese Christian church in Britain. Based upon twelve semi-structured interviews conducted with Christian leaders in nine cities, the research identifies the ways in which the COVID-19 outbreak is shaping the dynamics of intragroup and intergroup connectedness within and beyond the Chinese church in Britain. It finds that COVID-19 is playing a significant role in social connectedness. This manifests in three ways: the reconfiguration of a sense of belonging at church, the perception of outreach and evangelism, and the relationship between Chinese Christians from different regional backgrounds. These findings outline that the COVID-19 pandemic is triggering both cohesion and division. On the one hand, the outbreak is functioning as an incubator for a stronger sense of belonging to the church and appears to encourage the church to reach out to seekers and the wider community. On the other hand, the pandemic is also dividing the Chinese church through conflicts in political views and social attitudes. Such conflicts, which are primarily about democratic values and views of China's communist regime, are particularly observable between Mandarin-speaking Christians from mainland Chinese backgrounds and Cantonese-speaking Christians from Hong Kong backgrounds. The article argues that the coronavirus pandemic has initiated deeper reconstruction and reform in the Chinese Christian community in Britain in terms of organisation and mission

    Investigation of PtSGT1 and PtSGT4 Function in Cellulose Biosynthesis in Populus tomentosa Using CRISPR/Cas9 Technology

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    Cellulose synthesis is a complex process in plant cells that is important for wood processing, pulping, and papermaking. Cellulose synthesis begins with the glycosylation of sitosterol by sitosterol glycosyltransferase (SGT) to produce sitosterol-glucoside (SG), which acts as the guiding primer for cellulose production. However, the biological functions of SGTs in Populus tomentosa(P. tomentosa) remain largely unknown. Two full-length PtSGT genes (PtSGT1 and PtSGT4) were previously isolated from P. tomentosa and characterized. In the present study, CRISPR/Cas9 gene-editing technology was used to construct PtSGT1-sgRNA and PtSGT4-sgRNA expression vectors, which were genetically transformed into P. tomentosa using the Agrobacterium-mediated method to obtain transgenic lines. Nucleic acid and amino acid sequencing analysis revealed both base insertions and deletions, in addition to reading frame shifts and early termination of translation in the transgenic lines. Sugar metabolism analysis indicated that sucrose and fructose were significantly downregulated in stems and leaves of mutant PtSGT1-1 and PtSGT4-1. Glucose levels did not change significantly in roots and stems of PtSGT1-1 mutants; however, glucose was significantly upregulated in stems and downregulated in leaves of the PtSGT4-1 mutants. Dissection of the plants revealed disordered and loosely arranged xylem cells in the PtSGT4-1 mutant, which were larger and thinner than those of the wild-type. This work will enhance our understanding of cellulose synthesis in the cell walls of woody plants
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