Phosphomolybdic acid-responsive Pickering emulsions stabilized by ionic liquid functionalized Janus nanosheets

<p><b>A</b> Representative photomicrographs of Caspase-3 immunofluorescence staining (400×). <b>B</b> Quantification of Caspase-3 fluorescence intensity in different groups. <b>C</b> Representative Western blot band of Caspase-3 activation in the ischemic cortex at 24 h after reperfusion. <b>D</b> Effect of LBP (40 mg/kg) on the Caspase-3 activation in MCAO mice cortex at 24 h after reperfusion. Data are expressed as mean±SEM (n = 6). ^##P<0.01 vs. sham-operated group; **P<0.01 vs. vehicle group.</p

Effects of LBP on the expression of Cleaved PARP-1.

<p><b>A</b> Representative Western blot band of Cleaved PARP-1 activation in the ischemic cortex at 24 h after reperfusion. <b>B</b> Effect of LBP (40 mg/kg) on the Cleaved PARP-1 activation in MCAO mice cortex at 24 h after reperfusion. Data are expressed as mean±SEM (n = 6). ^##P<0.01 vs. sham-operated group; *P<0.05, **P<0.01 vs. vehicle group.</p

Effects of LBP on the expression of CytC.

<p><b>A</b> Representative photomicrographs of CytC immunofluorescence staining (400×). <b>B</b> Quantification of CytC fluorescence intensity in different groups. <b>C</b> Representative Western blot band of CytC activation in the ischemic cortex at 24 h after reperfusion. <b>D</b> Effect of LBP (40 mg/kg) on the CytC activation in MCAO mice cortex at 24 h after reperfusion. Data are expressed as mean±SEM (n = 6). ^##P<0.01 vs. sham-operated group; *P<0.05, **P<0.01 vs. vehicle group.</p

Effects of LBP on the expression of Bcl-2 protein.

<p><b>A</b> Representative photomicrographs of Bcl-2 immunofluorescence staining (400×). <b>B</b> Quantification of Bcl-2 protein fluorescence intensity in different groups. <b>C</b> Representative Western blot band of Bcl-2 protein expression in the ischemic cortex at 24 h after reperfusion. <b>D</b> Effect of LBP (40 mg/kg) on the Bcl-2 expression in MCAO mice cortex at 24 h after reperfusion. Data are expressed as mean±SEM (n = 6). ^#P<0.05, ^##P<0.01 vs. sham-operated group; **P<0.01 vs. vehicle group.</p

Effects of LBP (40 mg/kg) on the caspase-3 activities in left hemisphere after 2 h of MCAO and 24 h of reperfusion.

<p>Data are expressed as mean±SEM (n = 6). ^##P<0.01, vs. sham-operated group, *P<0.05 vs. vehicle group.</p

Protective effect of LBP against cerebral ischemic injury in MCAO mice brains.

<p><b>A</b> TTC staining of representative coronal sections at 24<b>B</b> Quantitative analysis of infarct volumes at 24 h after reperfusion. <b>C</b> Quantification of neurologic scores at 24 h after reperfusion. Data are expressed as mean±SEM (n = 6). ^##P<0.01, vs. sham-operated group, * P<0.05, **P<0.01 vs. vehicle group.</p

LBP reduces the number of Tunel positive neurons after focal cerebral ischemic injury.

<p><b>A</b> TUNEL staining of representative sections in mice ischemic penumbra of the cortex at 24<b>B</b> Quantitative analysis of apoptosis cells in cortex in different groups at 24 h after reperfusion. Data are expressed as mean±SEM (n = 6). ^##P<0.01 vs. sham-operated group; *P<0.05, **P<0.01 vs. vehicle group.</p

Effect of LBP treatment on morphological changes in ischemic penumbra of the cortex at 24-eosin staining (400×).

<p>A Sham-operated group. B Vehicle group. C LBP 10 mg/kg group. D LBP 20mg/kg group. E LBP 40mg/kg group. F Nimodipine 0.4mg/kg group.</p