Computer aided manual tracking

A scheme was developed to assist the human operator by augmenting an optic sight manual tracking loop with target rate estimates from a computer control algorithm which can either be a Kalman Filter or an alpha, beta, gamma filter. The idea is for the computer to provide rate tracking while the human operator is responsible for nullifying the tracking error. A simple schematic is shown to illustrate the implementation of this concept. A hybrid real-time man-in-loop simulation was used to compare the tracking performance of the same flight trajectory with or without this form of computer-aided track. Preliminary results show the advantage of computer-aided track against high speed aircraft at close range. However, good tracking before target state estimator maturity becomes more critical for aided track than without. Results are presented for a constant velocity flight trajectory

Integrate CRISPR/Cas9 for protein expression of HLA-B*38:68Q via precise gene editing.

The determination of null- or low-expressed HLA alleles is clinically relevant in both hematopoietic stem cell transplantation and solid organ transplantation. We studied the expression level of a questionable (Q) HLA-B*38:68Q allele, which carries a 9-nucleotide (nt) deletion at codon 230-232 in exon 4 of HLA-B*38:01:01:01 using CRISPR/Cas9 gene editing technology. CRISPR/Cas9 gene editing of HLA-B*38:01:01:01 homozygous EBV B cell line resulted in one HLA-B*38:68Q/B*38:01:01:01 heterozygous and one HLA-B*38:68Q homozygous clone. Flow cytometric analysis of monoclonal anti-Bw4 antibody showed the protein expression of HLA-B*38:01:01:01 in homozygous cells was 2.2 fold higher than HLA-B*38:68Q/B*38:01:01:01 heterozygous cells, and the expression of HLA-B*38:68Q/B*38:01:01:01 heterozygous cells was over 2.0 fold higher than HLA-B*38:68Q homozygous cells. The HLA-B*38:68Q expression was further confirmed using anti-B38 polyclonal antibody. Similarly, the expression of the HLA-B*38:01:01:01 homozygous cells was 1.5 fold higher than that of HLA-B*38:68Q/B*38:01:01:01 heterozygous cells, and the HLA-B*38:68Q/B*38:01:01:01 heterozygous cells was over 1.6 fold higher than that of HLA-B*38:68Q homozygous cells. The treatment of HLA-B*38:68Q homozygous cells with IFN-γ significantly increased its expression. In conclusion, we demonstrate that HLA-B*38:68Q is a low-expressing HLA allele. The CRISPR/Cas9 technology is a useful tool to induce precise gene editing in HLA genes to enable the characterization of HLA gene variants on expression and function

Generating EPR beams in a cavity optomechanical system

We propose a scheme to produce continuous variable entanglement between phase-quadrature amplitudes of two light modes in an optomechanical system. For proper driving power and detuning, the entanglement is insensitive with bath temperature and $Q$ of mechanical oscillator. Under realistic experimental conditions, we find that the entanglement could be very large even at room temperature.Comment: 4.1 pages, 4 figures, comments are welcome; to appear in PRA, published version with corrections of typo

“Passwords protect my stuff” - a study of children’s password practices

Children use technology from a very young age and often have to authenticate. The goal of this study is to explore children’s practices, perceptions, and knowledge regarding passwords. Given the limited work to date and that the world’s cyber posture and culture will be dependent on today’s youth, it is imperative to conduct cyber-security research with children. We conducted surveys of 189 3rd to 8th graders from two Midwest schools in the USA. We found that children have on average two passwords for school and three to four passwords for home. They kept their passwords private and did not share with others. They created passwords with an average length of 7 (3rd to 5th graders) and 10 (6–8th graders). But, only about 13% of the children created very strong passwords. Generating strong passwords requires mature cognitive and linguistic capabilities which children at this developmental stage have not yet mastered. They believed that passwords provide access control, protect their privacy and keep their “stuff” safe. Overall, children had appropriate mental models of passwords and demonstrated good password practices. Cyber-security education should strive to reinforce these positive practices while continuing to provide and promote age-appropriate developmental security skills. Given the study’s sample size and limited generalizability, we are expanding our research to include children from 3rd to 12th graders across multiple US school districts

Competing Phases, Strong Electron-Phonon Interaction and Superconductivity in Elemental Calcium under High Pressure

The observed "simple cubic" (sc) phase of elemental Ca at room temperature in the 32-109 GPa range is, from linear response calculations, dynamically unstable. By comparing first principle calculations of the enthalpy for five sc-related (non-close-packed) structures, we find that all five structures compete energetically at room temperature in the 40-90 GPa range, and three do so in the 100-130 GPa range. Some competing structures below 90 GPa are dynamically stable, i.e., no imaginary frequency, suggesting that these sc-derived short-range-order local structures exist locally and can account for the observed (average) "sc" diffraction pattern. In the dynamically stable phases below 90 GPa, some low frequency phonon modes are present, contributing to strong electron-phonon (EP) coupling as well as arising from the strong coupling. Linear response calculations for two of the structures over 120 GPa lead to critical temperatures in the 20-25 K range as is observed, and do so without unusually soft modes.Comment: 8 pages, 6 figures, 1 table, accepted for publication in Phys. Rev.