Utjecaj obiteljske mediteranske vrućice na ishod izvantjelesne oplodnje: retrospektivna analiza niza slučajeva

Although the in vitro fertilization-intra-cytoplasmic sperm injection (IVF-ICSI) has been utilized widely, the management in patients with an autoimmune disease is still a challenge. The aim of this study was to demonstrate IVF-ICSI outcomes in infertile women with familial Mediterranean fever (FMF). Patient data were collected from the cases registered from January 2006 until January 2014. A total of 6152 assisted reproductive technology (ART) cycles were analyzed retrospectively in the Ankara Zekai Tahir Burak Women’s Health Education and Research Hospital. Ten infertile women with FMF were included in the study. Baseline clinical and laboratory characteristics were collected and perinatal outcomes evaluated. The mean age (years), duration of infertility (years) and body mass index (kg/m2) were 29.9±5.3, 5.7±5.3 and 27.9±5.7, respectively. The mean baseline follicle-stimulating hormone (FSH; IU/L), estradiol (E2; pg/mL) and antral follicle count were 7.0±2.4, 48.1±15.8 and 7.9±2.9, respectively. The distribution of ovarian response was heterogeneous. Fourteen cycles in ten patients were evaluated. Embryo transfer could be achieved in only ten cycles. Three out of ten patients became pregnant. No adverse perinatal outcome was observed. Our findings indicate that FMF might have no impact on ART cycles.Iako je metoda oplodnje in vitro i intracitoplazmatskog injektiranja sperme (in vitro fertilization-intra-cytoplasmic sperm injection, IVF-ICSI) danas široko rasprostranjena u čitavom svijetu, njezina primjena u bolesnica s autoimunim bolestima još uvijek predstavlja velik izazov. Cilj ovoga istraživanja bio je pokazati ishode IVF-ICSI kod neplodnih žena s obiteljskom mediteranskom groznicom (familial Mediterranean fever, FMF). Podaci bolesnica prikupljeni su iz registriranih slučajeva od siječnja 2006. do siječnja 2014. godine. Retrospektivna analiza provedena u Zekai Tahir Burak Women’s Health Education and Research Hospital u Ankari obuhvatila je 6152 ciklusa potpomognute oplodnje (assisted reproductive technology, ART). U istraživanje je bilo uključeno deset neplodnih žena s FMF. Uzeti su bazalni podaci o kliničkim i laboratorijskim značajkama uključenih žena, kao i perinatalnim ishodima. Srednje vrijednosti dobi (godine), trajanja neplodnosti (godine) i indeksa tjelesne mase (kg/m2) bile su 29,9±5,3; 5,7±5,3 odnosno 27,9±5,7. Srednje vrijednosti folikul-stimulirajućeg hormona (FSH; IU/L), estradiola (E2; pg/mL) i broja antralnih folikula bile su 7,0±2,4; 48,1±15,8 odnosno 7,9±2,9. Raspodjela ovarijskog odgovora bila je heterogena. Procijenjeno je 14 ciklusa u deset žena. Transfer embrija bio je moguć u samo deset ciklusa, a trudnoća je postignuta u tri od deset žena. Nije zabilježen nikakav štetni perinatalni ishod. Naši nalazi ukazuju na to da FMF možda nema nikakvog utjecaja na cikluse ART

Investigation of the role of serum telomerase levels in patients with occult primary ovarian insufficiency: a prospective cross-sectional study

This study was designed to investigate serum telomerase levels of occult primary ovarian insufficiency (POI) and the relationship between in vitro fertilisation (IVF) results of these patients and serum telomerase levels. A cross-sectional case–control study was conducted between May and October 2017 including 78 patients at University of Health Science, Turkey. Occult POI was defined as women with a history of follicle-stimulating hormone (FSH) elevation between 12 and 25 IU/L and low ovarian reserve before initiation of IVF (n = 39). The control group were patients attending the hospital for contraception, with no history of infertility, having at least one healthy child (n = 39). Telomerase levels in serum samples were determined using enzyme-linked immunosorbent assay method. There was no statistically significant difference in serum telomerase levels in occult POI patients when compared with the control group.Impact statement What is already known on this subject? Clinical studies investigating the role of telomerase on reproductive function and in vitro fertilisation (IVF) outcomes in occult primary ovarian insufficiency (POI) patients are limited with no clear consensus and in all these studies polymerase chain reaction technique was used to evaluate telomere length. Regarding our knowledge, this is the first study in the literature investigating the role of serum telomerase levels in occult POI patients. What do the results of this study add? In contrast to the previous studies, in this study no statistically significant difference was found in serum telomerase levels in occult POI patients when compared with the fertile control patients. What are the implications of these findings for clinical practice and/or further research? The occult POI patients examined in this study are overlooked until they apply with infertility. Serum telomerase measurement is not useful to support the diagnosis of occult POI. Nevertheless, in order to confirm these findings, further studies in larger populations are needed

Sexual Dysfunction in Postpartum Turkish Women: It’s Relationship with Depression and Some Risk Factors

This study aimed to determine the prevalence of sexual dysfunction in postpartum Turkish women, and the relation between sexual dysfunction and depression, and some risk factors. This study was conducted with 530 postpartum women who had given birth during the previous 2-12 months. Data were collected with the Personal Information Form, Index of Female Sexual Function, and Edinburg Postnatal Depression Scale. For the data analysis, descriptive statistics, the Chi-square test, and Pearson correlation analysis were conducted using SPSS version 16. In the study, 74.3% of the postpartum women experienced sexual dysfunction. Sexual dysfunction was more prevalent in women who were high school graduates, whose reported economic status was middle class, who had a history of high-risk pregnancy, and whose menstruations did not recommence. It was also prevalention women who experienced dyspareunia, who did not use a family planning method, who used withdrawal family planning method, and who experienced postnatal depression. A medium correlation was determined between the sexual dysfunction and postnatal depression. Assessment of the sexual dysfunction prevalence and risk factors of postpartum women enables healthcare professionals to use necessary intervention strategies. Keywords: Depression, Postpartum period, Sexual dysfunction, Turkish wome

Relations between human leukocyte antigens and autoimmune hepatitis in Turkish children

Background/aims: We aimed to identify the genetic factors associated with increased tendency toward autoimmune hepatitis, a chronic and progressive inflammatory condition. Methods: A total of 32 children diagnosed with autoimmune hepatitis were included in the present study, and 160 healthy adult blood donors served as controls. In both groups, HLA phenotypes were examined (HLA-A, B, C, DR, DQ) and compared. In addition, the association between the type of autoimmune hepatitis and HLA status was explored. Results: Compared to controls, patients with autoimmune hepatitis had increased frequencies of the following class 1 HLA antigens: A24.9 (28% vs. 9%, p=0.007), A26 (25% us. 3%, p < 0.001), A32 (34% vs. 4%, p < 0.001), B38 (9% vs. 0.6%, p=0.015), and B51 (16% us. 0%, p < 0.001). Among class II HLA antigens, DRB1*04 (22% vs. 0%, p < 0.001), DRB1*07 (9% vs. 0%, p=0.004), DRB1*11 (12% us. 0%, p=0.001), DRB1*15 (25% vs. 0%, p < 0.001), DRB1*14 (31% us. 0%, p < 0.001), and DR11.5 (9% vs. 0%, p=0.004) were more frequent in patients compared to controls. Type 1 autoimmune hepatitis was associated with high frequencies of A24.9, A26, A32, and DRB1*15, whereas type 2 autoimmune hepatitis was associated with high frequencies of A26, B51, B38, and DRB1*11. On the other hand, frequencies of A32 and DRB1*04 were high among patients with unclassified autoimmune hepatitis. Conclusions: There seem to be associations between certain HLA antigens and susceptibility to autoimmune hepatitis, but variations among different geographical locations suggest a role for environmental factors

Effect of familial mediterranean fever on ivf outcome: a retrospective case series

Although the in vitro fertilization-intra-cytoplasmic sperm injection (IVF-ICSI) has been utilized widely, the management in patients with an autoimmune disease is still a challenge. The aim of this study was to demonstrate IVF-ICSI outcomes in infertile women with familial Mediterranean fever (FMF). Patient data were collected from the cases registered from January 2006 until January 2014. A total of 6152 assisted reproductive technology (ART) cycles were analyzed retrospectively in the Ankara Zekai Tahir Burak Women’s Health Education and Research Hospital. Ten infertile women with FMF were included in the study. Baseline clinical and laboratory characteristics were collected and perinatal outcomes evaluated. The mean age (years), duration of infertility (years) and body mass index (kg/m2) were 29.9±5.3, 5.7±5.3 and 27.9±5.7, respectively. The mean baseline follicle-stimulating hormone (FSH; IU/L), estradiol (E2; pg/mL) and antral follicle count were 7.0±2.4, 48.1±15.8 and 7.9±2.9, respectively. The distribution of ovarian response was heterogeneous. Fourteen cycles in ten patients were evaluated. Embryo transfer could be achieved in only ten cycles. Three out of ten patients became pregnant. No adverse perinatal outcome was observed. Our findings indicate that FMF might have no impact on ART cycles

The Effect of Leukospermia on Sperm Parameters in Couples With Unexplained Infertility

Amaç: Seminal sıvıdaki lökositler ile semen kalitesi arasındaki ilişki literatürde hala bir tartışma konusudur. Bu çalışmada amaç, açıklanamayan infertil hasta grubunda lökosperminin semen parametreleri üzerine etkilerini araştırmaktır. Materyal Metod: Haziran 2011 ile Aralık 2011 tarihleri arasında Ankarada üçüncü basamak bir eğitim araştırma hastanesinin tüp bebek kliniğinde yapılan semen analizleri retrospektif olarak incelendi. Açıklanamayan infertilite nedeni ile başvuran çiftlerden erkeğe ait sonuçlar bu veriler arasından seçildi. Toplamda intrauterine inseminasyon ve in-vitro fertilizasyon siklusları için hazırlanmış olan 1637 semen analizi çalışmaya dahil edildi. Azospermik ve oligospermik erkekler çalışma dışı bırakıldı. Lökospermi saptanan 348 semen analizi ile (Grup I) saptanmayan 1289 semen analizi sonucu (Grup II) karşılaştırıldı. Sonuç: Lökospermi (106 /mL) 348 hastada tespit edildi. Prevelansı toplamda %21.2 olarak değerlendirildi. İki grup arasında yaş, semen motilite ve morfolojisi açısından herhangi bir fark saptanmadı. (p0.05) Semen hacmi, pH, konsantrasyon ve total sperm sayısı açısından ise iki grup arasındaki farkı istatistiksel olarak anlamlı olduğu izlendi. (p0.05) Tartışma: Bu çalışma sonuçları, açıklanamayan infertilite nedeni ile başvuran çiftlerin erkek partnerlerinde lökospermin sık olarak izlendiğini göstermiştir. Motilite ve morfoloji lökospermiden etkilenmiyor gibi görünse de, semen hacmi, sperm konsantrasyonu ve total sperm sayısı üzerine etkisi nedeniyle açıklanamayan infertile hasta grubunda lökospermi detaylı bir araştırmayı hak etmektedir.Objective: The association between seminal leukocytes and semen quality is still a matter of debate in the literature. The purpose of this study was to evaluate the effect of leukospermia on semen parameters in unexplained infertile men. Materials and Methods: Semen analysis performed between June 2011 and December 2011 at infertility and in-vitro fertilization (IVF) clinic of a tertiary research and education hospital in Ankara were studied retrospectively. Results of the male partners of the couples with unexplained infertility were selected. A total of 1637 semen analysis of intrauterine insemination and in-vitro fertilization cycles were included in the study. Results of oligospermic and azospermic males were excluded. Semen parameters of 348 men with leukospermia (Group I) and 1289 without leukospermia (Group II) were compared. Results: Leukospermia (0.05). Conclusion: The results of this study showed that leukospermia occurs frequently in in male partners of couples with unexplained infertility. Although motility and morphology do not seem to be influenced by leukospermia, it de