PRUNUS SPINOSA L. VE RUBUS SANCTUS SCHREB. YAPRAK EKSTRAKTLARININ CAPSICUM ANNUUM L. ÇEŞİTLERİNDE TOPLAM PROTEİN MİKTARI VE PEROKSİDAZ AKTİVİTESİ ÜZERİNE ETKİLERİNİN BELİRLENMESİ

Prunus spinosa L. and Rubus sanctus Schreb. are species which belong to Rosaceae family, naturally occurring in Turkey. In this research, it was determined that leaves extracts of P. spinosa L. and R sanctus Schreb. which prepared with different solvents, stimulated the defense system of the grossum and longum varieties of Capsicum annuum L. at different levels within the scope of total protein amount and peroxidase activity according to the control group. In conclusion, it was shown that P. spinosa and R. sanctus species could be used as natural plant activators.Rosaceae familyasına ait olan Prunus spinosa L. ve Rubus sanctus Schreb. ülkemizde doğal yayılış gösteren bitki türleridir. Araştırmada farklı çözgenler ile hazırlanmış olan P. spinosa ve R. sanctus ekstraktlarının Capsicum annuum L. türünün grossum ve longum varyetelerinin savunma sistemini kontrol grubuna göre toplam protein miktarı ve peroksidaz aktivitesi kapsamında farklı düzeylerde uyardığı belirlenmiştir. Araştırmada doğal bitki aktivatörü geliştirilmesine yönelik çalışmalarda P. spinosa ve R. sanctus türlerinden yararlanılabileceği sonucuna varılmıştır

EFFECTS OF BLACKBERRY AND BLACKTHORN FRUIT EXTRACTS ON PROTEIN AND PEROXIDASE ACTIVITY IN PEPPER

In this research, blackthorn (Prunus spinosa L.) and blackberry (Rubus sanctus Schreb.) fruits have been extracted with water, ethanol, ethanol/water solvents. Fruit extracts were sprayed on the leaves of grossum and conoides varieties of Capsicum annuum L.. 24 and 48 hours after extract applications, healthy leaf of ten weeks old seedlings were harvested for protein and peroxidase analyses. Changing in total protein levels and peroxidase activity were measured spectrophotometrically.The highest total protein level increase was in the application of ethanolic extract of R. sanctus to grossum variety when we compare with control group. This increasing 24 and 48 hours after application were determined as 154% and 144% respectively. After the applications of R. sanctus fruit extract to the grossum and conoides varieties, peroxidase (POX) [EC 1.11.1.7] activity changing have been found better than P. spinosa extract application. After the both fruit extract applications, increasing in the POX activity of conoides variety have been found better than the grossum variety. 24 and 48 hours after application of ethanolic R. sanctus extract to conoides variety, POX activity increasing have been found respectively as 76% and 94%. In conclusion, it was shown that P. spinosa and R. sanctus fruit extracts have been stimulated the plant defense system in grossum and conoides varieties of C. annuum at different levels within the scope of total protein amount and peroxidase activity according to the control group