A multicentric evaluation of dipstick test for serodiagnosis of visceral leishmaniasis in India, Nepal, Sri Lanka, Brazil, Ethiopia and Spain

Author Correction: A multicentric evaluation of dipstick test for serodiagnosis of visceral leishmaniasis in India, Nepal, Sri Lanka, Brazil, Ethiopia and Spain PMID: 33574485Visceral leishmaniasis (VL) is one of the leading infectious diseases affecting developing countries. Colloidal gold-based diagnostic tests are rapid tools to detect blood/serum antibodies for VL diagnosis. Lack of uniformity in the performance of these tests in different endemic regions is a hurdle in early disease diagnosis. This study is designed to validate a serum-based dipstick test in eight centres of six countries, India, Nepal, Sri Lanka, Brazil, Ethiopia and Spain with archived and fresh sera from 1003 subjects. The dipstick detects antibodies against Leishmania donovani membrane antigens (LAg). The overall sensitivity and specificity of the test with 95% confidence intervals were found to be 97.10% and 93.44%, respectively. The test showed good sensitivity and specificity in the Indian subcontinent (>95%). In Brazil, Ethiopia, and Spain the sensitivity and specificity of the dipstick test (83.78-100% and 79.06-100%) were better as compared to the earlier reports of the performance of rK39 rapid test in these regions. Interestingly, less cross-reactivity was found with the cutaneous form of the disease in Spain, Brazil, and Sri Lanka demonstrating 91.58% specificity. This dipstick test can therefore be a useful tool for diagnosing VL from other symptomatically similar diseases and against cutaneous form of leishmaniasis.S

A Ten-Year Trend of Cutaneous Leishmaniasis at University of Gondar Hospital, Northwest Ethiopia: 2009-2018

Background. Cutaneous leishmaniasis (CL), which is one form of leishmaniasis, may show variations over years across regions, and epidemiological studies are crucial to estimate the cases of the disease status over a long time. This study is aimed at determining the trend of CL among patients at the University of Gondar Leishmaniasis Research and Treatment Center, northwest Ethiopia between 2009 and 2018 years. Methods. A ten-year data were extracted retrospectively. We included all patients who were visiting the center for CL diagnosis during the last ten years. Giemsa-stained skin slit microscopy was used to diagnose the disease. A chi-square test was used to compare the proportions of patients infected across years, seasons, months, sex, and age groups. Result. During the 10 years, a total of 1079 samples were requested for the diagnosis of CL. The cumulative average annual prevalence was found to be 55.4% (598/1079). The highest and lowest proportions of CL cases were recorded in 2014 (69.5%) and 2018 (35.4%), respectively. However, the percentage of CL cases did not show any significant differences over the study period. The number of suspected patients was significantly increased over the years (being lowest in 2009 and highest in 2017). The proportion of CL cases showed a remarkable difference across months but not seasons. CL was the highest within 15-49 years of age and males. Conclusion. The prevalence of CL did not show any significant differences over the last ten years. However, a remarkable increase of CL suspected cases was observed across the years. The disease showed significant association with age, sex, and months, but not seasons

Persistent T cell unresponsiveness associated with chronic visceral leishmaniasis in HIV-coinfected patients

<p><strong>Data for "Persistent T cell unresponsiveness associated with chronic VL disease in VL-HIV patients"</strong></p> <p><strong>Data directory</strong></p> <p>Contains processed single-cell RNA and TCR sequencing of two individuals per group. Patient and grouping information is provided in meta_data.txt</p> <p><strong>Script directories</strong></p> <ul> <li>src</li> </ul> <p>Contains various scripts used to generate the results and figures in the manuscript.</p> <p><strong>Result directories</strong></p> <ul> <li>results</li> </ul> <p>Contains the inbetween files for many of the analyses.<br> Contains the Seurat object for the single cell analysis reported in the paper.<br> Also used as output directory for the scripts listed in src, thus containing the figures used in the manuscript.</p&gt

Persistent T cell unresponsiveness associated with chronic VL disease in VL-HIV patients

Data for "Persistent T cell unresponsiveness associated with chronic VL disease in VL-HIV patients" Data directory Contains processed single-cell RNA and TCR sequencing of two individuals per group. Patient and grouping information is provided in meta_data.txt Script directories src Contains various scripts used to generate the results and figures in the manuscript. Result directories results Contains the inbetween files for many of the analyses. Contains the Seurat object for the single cell analysis reported in the paper. Also used as output directory for the scripts listed in src, thus containing the figures used in the manuscript