Detection of Low-Energy X-rays Using YSO Scintillation Crystal Arrays for GRB Experiments

We developed an X-ray detector using 36 arrays, each consisting of a 64-pixellated yttrium oxyorthosilicate (YSO) scintillation crystal and a 64-channel multi-anode photomultiplier tube. The X-ray detector was designed to detect X-rays with energies lower than 10 keV, primarily with the aim of localizing gamma-ray bursts (GRBs). YSO crystals have no intrinsic background, which is advantageous for increasing low-energy sensitivity. The fabricated detector was integrated into UBAT, the payload of the Ultra-Fast Flash Observatory (UFFO)/Lomonosov for GRB observation. The UFFO was successfully operated in space in a low-Earth orbit. In this paper, we present the responses of the X-ray detector of the UBAT engineering model identical to the flight model, using 241Am and 55Fe radioactive sources and an Amptek X-ray tube. We found that the X-ray detector can measure energies lower than 5 keV. As such, we expect YSO crystals to be good candidates for the X-ray detector materials for future GRB missions

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field