Screening And Detection Of Antiviral Activity In Alchornea Cordifolia Against Herpes Simplex Virus Type 2 In Vitro

Alchornea cordifolia, known as Pennah, has been used extensively as a medicinal plant in Africa for treating a variety of ailments. Crude water (H2O) and methanol (MeOH) extracts prepared from dried leaves of Pennah were tested for antiviral activity against Herpes simplex virus type 2 (HSV2) by plaque reduction assays, showing crude MeOH more effective as an antiviral against HSV2. Assays with fractionated MeOH extract resulted in highest plaque reduction in the 30% MeOH fraction (30% Pennah MeOH) at a significant 98% reduction at 250 µg/mL. Crude and 30% Pennah MeOH were found to contain alkaloids, phenols, and terpenoids. Immunofluorescence technique was utilized to visualize the extent viral products were synthesized in infected Vero cells treated with 30% Pennah MeOH extract according to different times of infection. Indirect immunofluorescence assays used to observe the course of HSV2 infection in the Vero cells in conjunction with time-sensitive and neutralization assays uncovered the extract did not prevent total viral attachment, but showed inhibition of viral replication. The specific step after viral attachment and penetration inhibited is still unknown, requiring further investigation. Time sensitive assays suggest extract must be in the presence of unattached viral particle to inhibit the viral replication due to decreased plaque reduction when extract is removed in comparison to applied to the cells at 2 and 8 hours post attachment. Overall, Alchornea cordifolia exhibited antiviral activity against HSV2, having the highest potential for plaque reduction in the 30% Methanol fraction, yet the inhibited events following attachment are still undefined

Session 2: Remembering Jimmy

10:30 a.m. — Session 2: Remembering Jimmy Stephen Yenser, distinguished professor of English, UCLA — Reading an essay about his friendship with Merrill Randy Bean, board member, James Merrill House Committee — Presenting on the history and initiatives of the James Merrill House Judith Moffett, adjunct professor emerita of English, University of Pennsylvania — Mixed Messages, an excerpt from Unlikely Friends: A Memoir Rachel Hadas, professor of English, Rutgers University — (via prerecorded video) reading an excerpt from The Book of Ephraim, reading her poem, Threshold and Mirror: the Biography, and recollecting her friendship with Merril

Pretemporada y rendimiento físico del equipo de futbol de la UNJFSC de la Liga de Huacho, 2022

La investigación de pretemporada en el fútbol es importante porque de ella depende el buen rendimiento físico de un jugador durante la competencia y sobre todo que sirve para evitar el riesgo de lesiones y tener un bajón de jugadores a causa de lesiones y en las fechas importantes o en disputa del título solo cuente con medio equipo titular porque el resto están en recuperación, de esa manera se hace poco probable conseguir el objetivo planteado al iniciar un torneo. Objetivo: Determinar la relación entre pretemporada con el rendimiento físico del equipo de fútbol de la UNJFSC de la liga de Huacho, 2022. Metodología, Tipo Descriptivo correlacional, un instrumento es el test para medir el rendimiento en el fútbol, población y muestra de 18 jugadores del equipo de UNJFSC de la liga de Huacho, 2022. Conclusiones: acepta la hipótesis alternativa que Existe relación significativa entre la pretemporada y rendimiento físico del equipo de fútbol UNJFSC de la liga de Huacho, 2022

Witness: The Modern Writer as Witness

Editor\u27s Note [Excerpt] Magic can mean many different things, especially for writers. Magic can be an illusion, a sleight of hand designed to trick onlookers into believing the impossible. Or magic can be a supernatural force in a world of harsh reality, a set of beliefs that sits just outside the realms of organized religion and advanced technology. Wizards and demons, Las Vegas entertainers and houngans --they all practice a kind of sorcery. For poets and prose writers, though, magic affords an opportunity for us to stretch the limitations of the physical world in search of new themes, settings, and characters. Magic is a door we eagerly walk through to reach new lands. We at Witness have thoroughly enjoyed the process of selecting the themed works we have collected here, mainly because the idea of enchantment is inspiring. There is the possibility of positive charms; there is a chance for dark witchery. And sometimes the spell cast by a character is nebulous, difficult to categorize. It’s arguable that we cherish these incantations the most, since they leave us in a state of wonderment bordering on disorientation. Yes, magic can also leave us bewildered and thankful for the bewilderment.https://digitalscholarship.unlv.edu/witness/1001/thumbnail.jp

The Anticipated Negative Impact on Emergency Medicine Faculty of the New ACGME Common Program Requirements (Poster).

Objectives: To determine, via EM faculty perceptions, the impact of the new CPR on their well-being and job satisfaction. The faculty were asked to anticipate the impact on the educational experience of residents