Multi-dark-state resonances in cold multi-Zeeman-sublevel atoms

We present our experimental and theoretical studies of multi-dark-state resonances (MDSRs) generated in a unique cold rubidium atomic system with only one coupling laser beam. Such MDSRs are caused by different transition strengths of the strong coupling beam connecting different Zeeman sublevels. Controlling the transparency windows in such electromagnetically induced transparency system can have potential applications in multi-wavelength optical communication and quantum information processing.Comment: 11pages, 4figure

Discovery of Diverse Rodent and Bat Pestiviruses With Distinct Genomic and Phylogenetic Characteristics in Several Chinese Provinces

Bats and rodents are widely distributed worldwide and can be native or intermediate reservoirs of many important zoonotic viruses. Pestiviruses are a group of virus species of the genus Pestivirus under the family Flaviviridae that can infect a wide variety of artiodactylous hosts, including swine and ruminants. Two classic types of pestiviruses, bovine viral diarrhea virus and classical swine fever virus, are important causative agents of mild-to-severe disease in bovine and swine hosts, respectively, and cause tremendous economic losses in these industries. Recent reports revealed that bats and rodents could also act as natural hosts of pestiviruses and an atypical porcine pestivirus, which cause disease in piglets, showed a close genetic relationship with a specific bat pestivirus, RaPestV-1. This study aimed to describe the detection and characterization of novel pestiviruses from bats and rodents in different locations by analyzing the available bat and rodent virome data from throughout China. Two bat pestivirus species and four rodent pestivirus species that are distinct from other known viruses were identified and sequenced. These viruses were identified from two bat species and four rodent species in different Chinese provinces. There were two distinct lineages present in these viruses, that differ from artiodactylous pestivirus. These findings expand our understanding of the genetic diversity of pestiviruses in bats and rodents and suggest the presence of a diverse set of pestiviruses in non-artiodactylous hosts. This study may provide new insight for the prevention of future viral disease outbreaks originating from bats and rodents

A tissue-specific landscape of sense/antisense transcription in the mouse intestine

<p>Abstract</p> <p>Background</p> <p>The intestinal mucosa is characterized by complex metabolic and immunological processes driven highly dynamic gene expression programs. With the advent of next generation sequencing and its utilization for the analysis of the RNA sequence space, the level of detail on the global architecture of the transcriptome reached a new order of magnitude compared to microarrays.</p> <p>Results</p> <p>We report the ultra-deep characterization of the polyadenylated transcriptome in two closely related, yet distinct regions of the mouse intestinal tract (small intestine and colon). We assessed tissue-specific transcriptomal architecture and the presence of novel transcriptionally active regions (nTARs). In the first step, signatures of 20,541 NCBI RefSeq transcripts could be identified in the intestine (74.1% of annotated genes), thereof 16,742 are common in both tissues. Although the majority of reads could be linked to annotated genes, 27,543 nTARs not consistent with current gene annotations in RefSeq or ENSEMBL were identified. By use of a second independent strand-specific RNA-Seq protocol, 20,966 of these nTARs were confirmed, most of them in vicinity of known genes. We further categorized our findings by their relative adjacency to described exonic elements and investigated regional differences of novel transcribed elements in small intestine and colon.</p> <p>Conclusions</p> <p>The current study demonstrates the complexity of an archetypal mammalian intestinal mRNA transcriptome in high resolution and identifies novel transcriptionally active regions at strand-specific, single base resolution. Our analysis for the first time shows a strand-specific comparative picture of nTARs in two tissues and represents a resource for further investigating the transcriptional processes that contribute to tissue identity.</p

Involvement of argonaute proteins in gene silencing and activation by RNAs complementary to a non-coding transcript at the progesterone receptor promoter

Double-stranded RNAs that are complementary to non-coding transcripts at gene promoters can activate or inhibit gene expression in mammalian cells. Understanding the mechanism for modulating gene expression by promoter-targeted antigene RNAs (agRNAs) will require identification of the proteins involved in recognition. Previous reports have implicated argonaute (AGO) proteins, but identifications have differed with involvement of AGO1, AGO2, or both AGO1 and AGO2 being reported by different studies. The roles of AGO3 and AGO4 have not been investigated. Here, we examine the role of AGO 1–4 in gene silencing and activation of the progesterone receptor (PR) gene. Expression of AGO2 is necessary for efficient gene silencing or activation and AGO2 is recruited to the non-coding transcript that overlaps the promoter during both gene silencing and activation. Expression of AGO1, AGO3 and AGO4 are not necessary for gene silencing or activation nor are AGO1, AGO3, or AGO4 recruited to the target non-coding transcript during gene activation. These data indicate that AGO2 is the primary AGO variant involved in modulating expression of PR by agRNAs

The Prevalence and Regulation of Antisense Transcripts in Schizosaccharomyces pombe

A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe

Research on tourism ecological safety evaluation of Huizhou Cultural and ecological reserve based on entropy -TOPSIS

Tourism ecological security is the basic guarantee for the sustainable development of tourist sites, Huizhou Cultural and Ecological Reserve is an important area for the im-plementation of ecological protection in China, and it is of great significance to carry out research on tourism ecological security. The study adopted the DPSIR model to construct a comprehensive evaluation index system for tourism ecological security and used entropy value-TOPSIS and ArcGIS software to analyze the inter-annual changes and spatial change characteristics of tourism ecological security in the study area. The results show that: firstly, the comprehensive index of tourism ecological safety in the study area from 2010 to 2021 shows a trend of “decreasing-increasing'' and an overall increasing trend; secondly, all the sub-systems show an increasing or stabilizing state in recent years during 2010–2021; the state and response sub-systems show an increas-ing or stabilizing state in recent years; and the state and response sub-systems show an increasing trend in recent years. Secondly, all the subsystems showed an increase or stabilization in recent years between 2010 and 2021, and the state and response sub-systems are the main systems to improve the ecological safety of tourism in the study area; thirdly, the difference in the level of ecological safety of tourism in each county of the study area increased and then narrowed from 2010 to 2021, and the change of safety level usually shifted between neighboring levels, and the probability of transfer-ring across the levels was relatively small. , Shexian County, Yixian County, Qimen County, Tunxi District, and the tourism eco-safety level of Huangshan District, Hui-zhou District, Jixi County, and Xiuning County increased at a faster rate than other counties. The study further extends the scale to the district and county level, tries to explore the relevant factors affecting the ecological security of tourism, and proposes countermeasures for the sustainable development of the study area based on the re-sults, which will bring some reference value to the ecological governance and policy formulation of this kind of research

Study on comprehensive evaluation of human settlements quality in Qinghai Province, China

The rapid development of modern industrial Civilization has put remote areas of China, especially Qinghai, in an increasingly unfavorable development position, and the problem of unbalanced and insufficient development in the Qinghai region has become increasingly serious, with environmental problems becoming increasingly prominent. Hence, this study is dedicated to assessing the quality of habitat in Qinghai, China. The effects of the evaluation of the Habitat Index will, to a sure extent, grant a reference for the current improvement purpose of harmonious coexistence between human beings and nature. Using the 2010–2020 Provincial data of Qinghai, China, and based on the existing literature and data, we constructed the evaluation index system of Qinghai's habitat environment quality in four dimensions, namely, health and safety, social development, economic status and environmental health, respectively. Combined with the entropy-weight-TOPSIS evaluation model, the Habitat Index of Qinghai residents was measured. The changes of habitat environment in different years were determined. Finally, the distribution of the correlation of each index is analyzed through grayscale correlation and box line diagram. The study concludes that, first, in terms of the time dimension, the overall Habitat Quality Index of Qinghai shows a steady increase from 2010 to 2020. 2020 has the highest Habitat Index, followed by 2019 and 2018. The relative proximity values of Cifor these three years are 0.597, 0.547, and 0.509, respectively. Second, statistically, the per capita urban road area has the most significant impact on Qinghai's Habitat Environment Index. It indicates that we need to improve and plan the traffic environment in Qinghai to create a safe and efficient traffic order. In addition, we need to focus on environmental protection, improve the level of sewage treatment, focus on the protection of water resources, etc. Thirdly, among the dimensional indicators, the gray correlation coefficient of each secondary indicator is >0.7. The correlation between each dimensional indicator and the quality of Qinghai's habitat environment is high. The highest correlation is found for the secondary social development (0.841). It indicates that the focus of improving the quality of habitat environment is to improve a series of problems in social development and adjust the social development model. Constantly optimizing social production and distribution, rationalizing resource utilization and development, etc

A Study of Factors Influencing the Use of the DingTalk Online Lecture Platform in the Context of the COVID-19 Pandemic

Online classes quickly became a hot topic in education during the effort to prevent and manage the COVID-19 outbreak. This paper is of great value in analyzing the factors influencing online delivery from a socially acceptable perspective, using the online DingTalk platform course as the research target. The researchers of this paper used a questionnaire to establish the conceptual basis of the survey based on the technology acceptance model (TAM), and developed the corresponding survey questions. The questionnaire was distributed to 528 respondents, of which 495 were valid data samples; furthermore, the sample efficiency of the returned questionnaire was 93.75%. The data were analyzed by SPSSAU software for reliability (Cronbach alpha: 0.967). For the purposes of assessing validity, ANOVA was used, and the SEM structural ANOVA was utilized in order to understand the impact of using the DingTalk platform for online classroom teaching, as well as to study user satisfaction with its use and to make relevant suggestions for continuing to use the online platform for classes in terms of campus management for online/offline hybrid teaching

A comprehensive dataset of animal-associated sarbecoviruses

Abstract Zoonotic spillover of sarbecoviruses (SarbeCoVs) from non-human animals to humans under natural conditions has led to two large-scale pandemics, the severe acute respiratory syndrome (SARS) pandemic in 2003 and the ongoing COVID-19 pandemic. Knowledge of the genetic diversity, geographical distribution, and host specificity of SarbeCoVs is therefore of interest for pandemic surveillance and origin tracing of SARS-CoV and SARS-CoV-2. This study presents a comprehensive repository of publicly available animal-associated SarbeCoVs, covering 1,535 viruses identified from 63 animal species distributed in 43 countries worldwide (as of February 14,2023). Relevant meta-information, such as host species, sampling time and location, was manually curated and included in the dataset to facilitate further research on the potential patterns of viral diversity and ecological characteristics. In addition, the dataset also provides well-annotated sequence sets of receptor-binding domains (RBDs) and receptor-binding motifs (RBMs) for the scientific community to highlight the potential determinants of successful cross-species transmission that could be aid in risk estimation and strategic design for future emerging infectious disease control and prevention

Panoramic analysis of coronaviruses carried by representative bat species in Southern China to better understand the coronavirus sphere

Abstract Bats, recognized as considerable reservoirs for coronaviruses (CoVs), serve as natural hosts for several highly pathogenic CoVs, including SARS-CoV and SARS-CoV-2. Investigating the bat CoV community provides insights into the origin for highly pathogenic CoVs and highlights bat CoVs with potential spillover risks. This study probes the evolution, recombination, host range, geographical distribution, and cross-species transmission characteristics of bat CoVs across China and its associated CoVs in other regions. Through detailed research on 13,064 bat samples from 14 provinces of China, 1141 CoV strains are found across 10 subgenera and one unclassified Alpha-CoV, generating 399 complete genome sequences. Within bat CoVs, 11 new CoV species are identified and 425 recombination events are detected. Bats in southern China, particularly in Yunnan province, exhibit a pronounced diversity of CoVs. Limited sampling and low detection rates exist for CoVs in Myotacovirus, Nyctacovirus, Hibecovirus, Nobecovirus in China. The genus Myotis is highlighted as a potential ancestral host for Alpha-CoV, with the genus Hipposideros suggested as a likely progenitor host for bat-associated Beta-CoV, indicating the complexity of cross-species transmission dynamics. Through the comprehensive analysis, this study enriches the understanding of bat CoVs and offers a valuable resource for future research