Investigations of urethral sphincter activity in mice with bladder hyperalgesia before and after drug administration of gabapentin.

PurposeThis study investigated the effect of gabapentin on lower urinary tract dysfunction focusing on urethral activities and cystitis-induced hyperalgesia in a mouse model of painful bladder syndrome/interstitial cystitis (PBS/IC). The electromyography (EMG) of external urethral sphincter (EUS) was difficult to obtain, but contained useful information to examine the drug effect in mice.MethodsFemale C57BL/6J mice were intraperitoneally (ip) administration with either saline or 200 mg/kg of cyclophosphamide (CYP) 48 h before experimental evaluation. Cystitis mice were treated with administration of gabapentin (25 or 50 mg/kg, ip). Cystometry and EUS EMG were obtained and analyzed during continuous bladder infusion. The visceral pain-related visceromotor reflex (VMR) was recorded in response to isotonic bladder distension.ResultsCystitis mice showed shorter inter-contraction intervals and increased occurrence of non-voiding contractions during bladder infusion, with increased VMR during isotonic bladder distension, indicating cystitis-induced bladder hyperalgesia. Gabapentin (50 mg/kg) suppressed effects of CYP on cystometry, but not on EUS EMG activity, during bladder infusion. The effect on urodynamic recordings lasted 4 h. VMR was significantly reduced by gabapentin.ConclusionsThe present study showed that CYP-induced cystitis in mice is a model of visceral hyperalgesia affecting detrusor contractions, not urethral activations. The technique of using EUS EMG to evaluate the drug effects on urethral activities is novel and useful for future investigations. Gabapentin can be as a potential treatment for detrusor overactivity and PBS/IC

Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

Early detection of SARS-CoV in throat wash and saliva suggests that these specimens are ideal for SARS diagnosis

Telomeres and Telomerase in Cardiovascular Diseases

Telomeres are tandem repeat DNA sequences present at the ends of each eukaryotic chromosome to stabilize the genome structure integrity. Telomere lengths progressively shorten with each cell division. Inflammation and oxidative stress, which are implicated as major mechanisms underlying cardiovascular diseases, increase the rate of telomere shortening and lead to cellular senescence. In clinical studies, cardiovascular risk factors such as smoking, obesity, sedentary lifestyle, and hypertension have been associated with short leukocyte telomere length. In addition, low telomerase activity and short leukocyte telomere length have been observed in atherosclerotic plaque and associated with plaque instability, thus stroke or acute myocardial infarction. The aging myocardium with telomere shortening and accumulation of senescent cells limits the tissue regenerative capacity, contributing to systolic or diastolic heart failure. In addition, patients with ion-channel defects might have genetic imbalance caused by oxidative stress-related accelerated telomere shortening, which may subsequently cause sudden cardiac death. Telomere length can serve as a marker for the biological status of previous cell divisions and DNA damage with inflammation and oxidative stress. It can be integrated into current risk prediction and stratification models for cardiovascular diseases and can be used in precise personalized treatments. In this review, we summarize the current understanding of telomeres and telomerase in the aging process and their association with cardiovascular diseases. In addition, we discuss therapeutic interventions targeting the telomere system in cardiovascular disease treatments

Subsurface Structure, Physical Properties, and Fault Zone Characteristics in the Scientific Drill Holes of Taiwan Chelungpu-Fault Drilling Project

Continuous coring and a suite of geophysical measurements were collected in two scientific holes to understand physical mechanisms involved in large displacements in the 1999 Chi-Chi earthquake. Physical properties of formations obtained from wire-line logs including P- and S-wave sonic velocity, gamma ray, electrical resistivity, density and temperature, are primarily dependent on parameters such as lithology, depth and fault zones. The average dip of bedding, identified from both cores and FMI (or FMS) logs, is about 30 degrees towards the SE. Nevertheless, local azimuthal variations and increasing or decreasing bedding dips appear across fault zones. A prominent increase in structural dip to 60¢X_n- 80¢X_nbelow 1856 m could be due to deformation associated with propagation of the Sanyi fault

Estimating the Risk of River Flow under Climate Change in the Tsengwen River Basin

This study evaluated the overflow risk of the Tsengwen River under a climate change scenario by using bias-corrected dynamic downscaled data as inputs for a SOBEK model (Deltares, the Netherlands). The results showed that the simulated river flow rate at Yufeng Bridge (upstream), Erxi Bridge (midstream), and XinZong (1) (downstream) stations are at risk of exceeding the management plan’s flow rate for three projection periods (1979–2003, 2015–2039, 2075–2099). After validation with the geomorphic and hydrological data collected in this study, the frequency at which the flow rate exceeded the design flood was 2 in 88 events in the base period (1979–2003), 6 in 82 events in the near future (2015–2039), and 10 in 81 events at the end of the century (2075–2099)