Early detection of SARS-CoV in throat wash and saliva suggests that these specimens are ideal for SARS diagnosis

Chan-Ping Su

Chao-Fu Yang

Chi-Tai Fang

Chien-Ching Hung

Chuan-Liang Kao

Hsien-Ping Hu

Hui-Ling Chen

I-Jung Liu

Jann-Tay Wang

Jih-Hui Lin

Jin-Town Wang

Jyh-Yuan Yang

Li-Min Huang

null null

Pan-Chyr Yang

Pei-Jer Chen

Po-Ren Hsueh

Shan-Chwen Chang

Shey-Ying Chen

Shiou-Hwei Yeh

Szu-Chia Hsieh

Szu-Min Hsieh

Wang-Hwei Sheng

Wei-Kung Wang

Wen-Chu Chiang

Yee-Chun Chen

Yu-Ping Chiang

Emerging Infectious Diseases

English

PubMed

Wang, Wei-Kung

National Taiwan University Repository

Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis

Crossref

Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

The severe acute respiratory syndrome–associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 x 102 to 5.93 x 106 copies/mL) and saliva (7.08 x 103 to 6.38 x 108 copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets. Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four cases, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis

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Detection of SARS-associated Coronavirus in Throat Wash and Saliva in Early Diagnosis

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