Comparison of proteomic responses as global approach to antibiotic mechanism of action elucidation

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. New antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of the bottlenecks is the elucidation of targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for the rapid classification of compounds into those with precedented and unprecedented modes of action. We established a proteomic response library of Bacillus subtilis covering 91 antibiotics and comparator compounds, and a mathematical approach was developed to aid data analysis. Comparison of proteomic responses (CoPR) allows the rapid identification of antibiotics with dual mechanisms of action as shown for atypical tetracyclines. It also aids in generating hypotheses on mechanisms of action as presented for salvarsan (arsphenamine) and the antirheumatic agent auranofin, which is under consideration for repurposing. Proteomic profiling also provides insights into the impact of antibiotics on bacterial physiology through analysis of marker proteins indicative of the impairment of cellular processes and structures. As demonstrated for trans-translation, a promising target not yet exploited clinically, proteomic profiling supports chemical biology approaches to investigating bacterial physiology

Deaths in Turkish military services, 1998-2000

The aim of this study was to evaluate the causes of death in the military in Turkey and to evaluate the previously obtained data relating to the characteristics peculiar to the army in those cases of death that have acquired a juridical attribute. In this study, the cases presented in the years 1998 and 2000 have been investigated retrospectively. Seventy military cases investigated within a period of 3 years were all men. Except for the 20 cases in which the cause of death could not be determined, 30 of 50 cases in our study are included in the group of "natural deaths" and 20 are included in the group of "forced deaths." In conclusion, natural causes are still the leading cause of death in Turkey, and regarding this, it can be suggested that these deaths could be preventable by better health care and more careful premilitary health examinations

Fatalities due to methyl alcohol intoxication in Turkey: an 8-year study

The aim of this study is to examine methyl alcohol poisoning cases from the medico-legal point of view. The records of the Morgue Department of Council of the Forensic Medicine were reviewed retrospectively for all methyl alcohol poisonings for the period of 27.10.1992 and 30.05.2001. The victim's age, sex, death year, death place, methyl alcohol blood levels, the source of methyl alcohol, accompanying laboratory results and histopathologic tissue changes were recorded