Schizophrenia plausible protective effect of microRNA-137 is potentially related to estrogen and prolactin in female patients

BackgroundSchizophrenia (SCZ) is a serious chronic mental disorder. Our previous case–control genetic association study has shown that microRNA-137 (miR-137) may only protect females against SCZ. Since estrogen, an important female sex hormone, exerts neuroprotective effects, the relationship between estrogen and miR-137 in the pathophysiology of SCZ was further studied in this study.MethodsGenotyping of single-nucleotide polymorphism rs1625579 of miR-137 gene in 1,004 SCZ patients and 896 healthy controls was conducted using the iMLDR assay. The effect of estradiol (E2) on the miR-137 expression was evaluated on the human mammary adenocarcinoma cell line (MCF-7) and the mouse hippocampal neuron cell line (HT22). The relationships between serum E2, prolactin (PRL), and peripheral blood miR-137 were investigated in 41 SCZ patients and 43 healthy controls. The miR-137 and other reference miRNAs were detected by real-time fluorescent quantitative reverse transcription-PCR.ResultsBased on the well-known SNP rs1625579, the distributions of protective genotypes and alleles of the miR-137 gene were not different between patients and healthy controls but were marginally significantly lower in female patients. E2 upregulated the expression of miR-137 to 2.83 and 1.81 times in MCF-7 and HT22 cells, respectively. Both serum E2 and blood miR-137 were significantly decreased or downregulated in SCZ patients, but they lacked expected positive correlations with each other in both patients and controls. When stratified by sex, blood miR-137 was negatively correlated with serum E2 in female patients. On the other hand, serum PRL was significantly increased in SCZ patients, and the female patients had the highest serum PRL level and a negative correlation between serum PRL and blood miR-137.ConclusionThe plausible SCZ-protective effect of miR-137 may be female specific, of which the underlying mechanism may be that E2 upregulates the expression of miR-137. This protective mechanism may also be abrogated by elevated PRL in female patients. These preliminary findings suggest a new genetic/environmental interaction mechanism for E2/miR-137 to protect normal females against SCZ and a novel E2/PRL/miR-137-related pathophysiology of female SCZ, implying some new antipsychotic ways for female patients in future

Real-Time Detection of River Surface Floating Object Based on Improved RefineDet

Object detection is widely used in robot navigation, intelligent security and industrial detection, but it is rarely used in water conservancy industry. In the aspect of river and lake health management, it is important to clean out the floating objects in time, which prevents water pollution caused by the accumulation of the floating objects. The early methods of water surface object detection, such as background subtraction and frame difference, are greatly affected by the change of object shape and background, resulting in poor robustness. Therefore, we propose a real-time detection method of surface floating objects based on improved RefineDet model, which includes three modules: the anchor refinement module, the transfer connection block and the object detection module. We improve anchor refinement module by adding convolution layers to obtain higher-level semantic, and fuse high-level features with low-level features to improve detection accuracy. Moreover, we adjust the parameters setting of anchors according to the scale and aspect ratio distribution to match the multi-scale object better. Aiming at the foreground-background class imbalance caused by dense anchors sampling, we introduce focal loss function to solve it and make our model more efficient by adjusting the parameters of the function. We verify the performance of the proposed method on different floating object datasets we constructed. The detection accuracies on these different datasets are 83.8%, 88.0%, and 82.3% respectively, and the detection speed is 28 FPS. This shows that the improved RefineDet realizes high-precision and real-time detection

The uncanny valley effect in typically developing children and its absence in children with autism spectrum disorders.

Robots and virtual reality are gaining popularity in the intervention of children with autism spectrum disorder (ASD). To shed light on children's attitudes towards robots and characters in virtual reality, this study aims to examine whether children with ASD show the uncanny valley effect. We varied the realism of facial appearance by morphing a cartoon face into a human face, and induced perceptual mismatch by enlarging the eyes, which has previously been shown as an effective method to induce the uncanny valley effect in adults. Children with ASD and typically developing (TD) children participated in a two-alternative forced choice task that asked them to choose one they liked more from the two images presented on the screen. We found that TD children showed the effect, i.e., the enlargement of eye size and the approaching realism reduced their preference. In contrast, children with ASD did not show the uncanny valley effect. Our findings in TD children help resolve the controversy in the literature about the existence of the uncanny valley effect among young children. Meanwhile, the absence of the uncanny valley effect in children with ASD might be attributed to their reduced sensitivity to subtle changes of face features and their limited visual experience to faces caused by diminished social motivation. Last, our findings provide practical implications for designing robots and virtual characters for the intervention of children with ASD

Comparing the Water-holding Characteristics of Broadleaved, Coniferous, and Mixed Forest Litter Layers in a Karst Region

Karst forests are often located in mountainous regions, and because of various geological factors both soil and water loss are major conservation concerns. We investigated the water-holding characteristics of 3 typical karst forest types through field sampling and laboratory experiments. The results showed that (1) the total litter mass of the coniferous forest was significantly higher than that of either the mixed forest or the broadleaved forest; (2) the mass of semidecomposed litter was significantly higher than that of undecomposed litter; (3) the litter layers of the mixed and coniferous forests had similar maximum water-holding capacity, whereas the maximum water-holding capacity of the broadleaved forest was significantly lower; (4) the maximum water-retention capacity of both the mixed and coniferous forests was significantly higher than that of the broadleaved forest; and (5) water-absorption rate and maximum water-holding capacity varied significantly across forest and litter types, with the mixed forest and undecomposed litter layers tending both to hold more water and to absorb water more quickly than the other forest types or the semidecomposed litter layer. Because of the elevated water-holding capacity and absorption rate of the mixed forest in karst regions, special emphasis on the conservation of this complex forest ecosystem is critical from both hydrological and ecological perspectives