Mining the sequence data of Rhizobium Leguminosarum BV Trifolii WSM1325 and WM2304

Most clover rhizobial inoculants form effective nitrogen-fixing symbioses with either annual or perennial species (and very few with both). This constraint provides a considerable barrier to agricultural productivity since background populations of R. trifolii may nodulate with an incompatible host but ineffectively fix nitrogen (Yates et al 2008)

Direct seeding of chenopod shrubs for saltland and rangeland environments

There are currently two ways of establishing chenopod shrubs: sowing from seed using a niche seeder, or planting nursery-raised seedlings with a tree planter. Planting seedlings is the more reliable method, but is relatively expensive (in excess of $450 per hectare). On the other hand, direct seeding using the specialised “niche seeder” is much less expensive ($100-150 per hectare), but is also less reliable. This project aimed to investigate alternative methods of direct seeding chenopod shrubs for saltland and rangeland areas by developing a greater understanding of their seed biology and agronomic requirements. Our aspiration was that shrubs should be established using more conventional farm machinery. This bulletin reports on a combination of seed biology and agronomic research to develop reliable, low-cost direct seeding options for chenopod shrubs. Experiments into the impact of changing environmental conditions on seeds were studied in the laboratory, and field experiments were conducted to test the applicability of these insights in the field using conventional modified farm seeding machinery. As a result of this work, a successful direct seeding package using farm seeding equipment (modified for wide row spacings and depth control) was developed for Atriplex nummularia (old man saltbush), the most widely planted saltbush species across southern Australia. The nine key elements of the package are: 1. Select suitable paddocks for introduction of new shrubs 2. Prepare a weed-free seedbed using two knockdown herbicide applications (4-6 weeks and 1-2 weeks before seeding) and commence control of rabbits and kangaroos 3. Sow the best seed, by ensuring: a. Large fruits, with a high proportion of viable seeds, have been selected b. Seed is of subspecies nummularia (not subsp. spathulata) c. Fruits have been harvested within the previous six months and stored in a cool, dry environment d. Bracts are retained around the seeds 4. Sow into moisture in late winter - early spring (depending on district) a. If the area to be sown is waterlogged, defer sowing until later in spring b. If insufficient soil moisture, defer sowing until the following year 5. Use a sowing rate of ~10 fruits/m (if germination rate is 15%) to provide at least one plant for every 2 m of row; use higher rates for seed of lower germination 6. Set the seeder up to sow into furrows with trailing press wheels 7. Sow to a depth of 5-10 mm (very critical) 8. Control weeds and pests (insects, mites, kangaroos and rabbits) 9. Defer grazing until seedlings are well established This establishment method has also been shown to work for Rhagodia preissii (mallee saltbush). This project was not able to develop reliable direct seeding packages for other Atriplex species, including A. amnicola and A. undulata. Further work is needed to understand the triggers for their germination, before these species can be direct-seeded with conventional machinery. Direct sowing of M. brevifolia and M. pyramidata appears to be problematic in much of southern Australia, due to their requirement for temperatures >30°C for germination, which do not occur within the normal winter growing season. An exception to this would be areas with more reliable summer rainfall, such as northern New South Wales, where sowing could be deferred until late spring-early summer. An alternative strategy for establishing M. brevifolia, is to encourage natural recruitment of seedlings from seed produced on surrounding bushes (if it is already present in the area), or to transplant a low density of nursery-raised seedlings, which could then act as a seed source for natural recruitment (if it is not already present)

Complete genome sequence of the Medicago microsymbiont Ensifer (Sinorhizobium) medicae strain WSM419

Ensifer (Sinorhizobium) medicae is an effective nitrogen fixing microsymbiont of a diverse range of annual Medicago (medic) species. Strain WSM419 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from a M. murex root nodule collected in Sardinia, Italy in 1981. WSM419 was manufactured commercially in Australia as an inoculant for annual medics during 1985 to 1993 due to its nitrogen fixation, saprophytic competence and acid tolerance properties. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first report of a complete genome se-quence for a microsymbiont of the group of annual medic species adapted to acid soils. We reveal that its genome size is 6,817,576 bp encoding 6,518 protein-coding genes and 81 RNA only encoding genes. The genome contains a chromosome of size 3,781,904 bp and 3 plasmids of size 1,570,951 bp, 1,245,408 bp and 219,313 bp. The smallest plasmid is a fea-ture unique to this medic microsymbiont

Multibeam Maser Survey of methanol and excited OH in the Magellanic clouds: new detections and maser abundance estimates

‘The definitive version is available at www.blackwell-synergy.com.’ Copyright Blackwell Publishing DOI: 10.1111/j.1365-2966.2008.12888.xPeer reviewe

The Ginzburg regime and its effects on topological defect formation

The Ginzburg temperature has historically been proposed as the energy scale of formation of topological defects at a second order symmetry breaking phase transition. More recently alternative proposals which compute the time of formation of defects from the critical dynamics of the system, have been gaining both theoretical and experimental support. We investigate, using a canonical model for string formation, how these two pictures compare. In particular we show that prolonged exposure of a critical field configuration to the Ginzburg regime results in no substantial suppression of the final density of defects formed. These results dismiss the recently proposed role of the Ginzburg regime in explaining the absence of topological defects in 4He pressure quench experiments.Comment: 8 pages, 5 ps figure

A Grand Canonical Ensemble Approach to the Thermodynamic Properties of the Nucleon in the Quark-Gluon Coupling Model

In this paper, we put forward a way to study the nucleon's thermodynamic properties such as its temperature, entropy and so on, without inputting any free parameters by human hand, even the nucleon's mass and radius. First we use the Lagrangian density of the quark gluon coupling fields to deduce the Dirac Equation of the quarks confined in the gluon fields. By boundary conditions we solve the wave functions and energy eigenvalues of the quarks, and thus get energy-momentum tensor, nucleon mass, and density of states. Then we utilize a hybrid grand canonical ensemble, to generate the temperature and chemical potentials of quarks, antiquarks of three flovars by the four conservation laws of the energy and the valence quark numbers, after which, all other thermodynamic properties are known. The only seemed free paremeter, the nucleon radius is finally determined by the grand potential minimal principle.Comment: 5 pages, LaTe

The JCMT Legacy Survey of the Gould Belt: a first look at Orion B with HARP

‘The definitive version is available at www3.interscience.wiley.com '. Copyright Royal Astronomical Society.The Gould Belt Legacy Survey will survey nearby star-forming regions (within 500 pc), using Heterodyne Array Receiver Programme (HARP), Submillimetre Common-User Bolometer Array 2 and Polarimeter 2 on the James Clerk Maxwell Telescope. This paper describes the initial data obtained using HARP to observe 12CO, 13CO and C18O J= 3 → 2 towards two regions in Orion B, NGC 2024 and NGC 2071. We describe the physical characteristics of the two clouds, calculating temperatures and opacities utilizing all the three isotopologues. We find good agreement between temperatures calculated from CO and from dust emission in the dense, energetic regions. We determine the mass and energetics of the clouds, and of the high-velocity material seen in 12CO emission, and compare the relative energetics of the high- and low-velocity material in the two clouds. We present a clumpfind analysis of the 13CO condensations. The slope of the condensation mass functions, at the high-mass ends, is similar to the slope of the initial mass function.Peer reviewe