Asymmetric leaves2 and elongator, a histone acetyltransferase complex, mediate the establishment of polarity in leaves of Arabidopsis thaliana

Leaf primordia are generated around the shoot apical meristem. Mutation of the ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana results in defects in repression of the meristematic and indeterminate state, establishment of adaxial-abaxial polarity and left-right symmetry in leaves. AS2 represses transcription of meristem-specific class 1 KNOX homeobox genes and of the abaxial-determinant genes ETTIN/ARF3, KANADI2 and YABBY5. To clarify the role of AS2 in the establishment of leaf polarity, we isolated mutations that enhanced the polarity defects associated with as2. We describe here the enhancer-of-asymmetric-leaves-two1 (east1) mutation, which caused the formation of filamentous leaves with abaxialized epidermis on the as2-1 background. Levels of transcripts of class 1 KNOX and abaxial-determinant genes were markedly higher in as2-1 east1-1 mutant plants than in the wild-type and corresponding single-mutant plants. EAST1 encodes the histone acetyltransferase ELONGATA3 (ELO3), a component of the Elongator complex. Genetic analysis, using mutations in genes involved in the biogenesis of a trans-acting small interfering RNA (ta-siRNA), revealed that ELO3 mediated establishment of leaf polarity independently of AS2 and the ta-siRNA-related pathway. Treatment with an inhibitor of histone deacetylases (HDACs) caused additive polarity defects in as2-1 east1-1 mutant plants, suggesting the operation of an ELO3 pathway, independent of the HDAC pathway, in the determination of polarity. We propose that multiple pathways play important roles in repression of the expression of class 1 KNOX and abaxial-determinant genes in the development of the adaxial domain of leaves and, thus, in the establishment of leaf polarity

Efficient generation of an oxidopyrylium ylide using a Pd catalyst and its [5+2] cycloadditions with several dipolarophiles

An efficient method for the generation of an oxidopyrylium ylide from 6-acetoxy-6-acetoxymethyl-2H-pyran-3(6H)-one using a Pd catalyst and [5+2] cycloadditions of the resulting ylide are described. Among substituted styrene derivatives as dipolarophiles, electron-rich styrenes showed higher yield (up to 80%). The [5+2] cycloaddition reactions can also be applied to exo-methylene cyclic compounds, and an improved method for the synthesis of polygalolide intermediate has been demonstrated.ArticleCHEMICAL COMMUNICATIONS.54(9):1109-1112(2018)journal articl

BUCKLING OF AN ANNULAR SECTOR PLATE SUBJECTED TO IN-PLANE MOMENTS

"Buckling of an annular sector plate subjected to equal and opposite moments at the radial edges is examined. The governing differential equation of the plate is solved by a Galerkin method. Buckling moments and buckling modes are obtained for the annular sector plate with simply-supported radial edges and arbitrary boundary conditions along the circumferential edges.Numerical results are shown for various geometrical parameters and three different boundary conditions. Moreover，the buckling properties of an annular sector plate are compared with those of a rectangular plate and a circular beam.

Nonlinear Response of an Annular Sector Plate Subjected to In-Plane Dynamic Moment

The nonlinear dynamic instability of an annular sector plate subjected to equal and opposite time-varying moments is examined. The equation of motion describing a large deflection of the annular sector plate based upon Berger\u27s approximate equation is analyzed by the Galerkin method.The resulting equations for time variables are integrated by using the Runge-Kutta-Gill method. Numerical results are presented for various boundary conditions, damping forces, and static moments.Proceedings of the 38th Japan National Congress for Applied Mechanics, 198