Adventitious regeneration from haploid melon (Cucumis melo L.) leaves as an approach to increase the frequency of diploid plants

An efficient process of adventitious regeneration from haploid plant leaves to increase the proportion of diploid plants of six elite melon lines ‘Védrantais’ French cultivar, ‘Piel de Sapo’ Spanish line ‘T111’, and four near isogenic lines (NILs: SC 6–6, SC 7–2, SC 7–4, SC 8–4) has been developed. Several hormone combinations, in vitro culture conditions, and leaf positions on the plant stem were analyzed to improve the regeneration frequency and the percentage of diploid plants in these lines. Flow cytometry showed that diploid plant production was optimized by regeneration from haploid leaves as the percentage of diploid plants increased from 11% with regeneration of diploid cotyledons to 60% in this work. Evaluating the four NILs, only the lines SC 6–6 and SC 8–4 showed a significantly higher percentage of diploidy with 80% and 72.7%, respectively. The leaf position factor had no effect on regeneration and ploidy level, while darkness negatively influenced regeneration but had no effect on ploidy. Furthermore, it was concluded that the hormonal combination (cytokinin plus auxin) induces low endoreduplication activity, which is the phenomenon responsible for the polyploidy. Furthermore, this study provided evidence that in melon polyploidy occurs during the process of organogenesis, and, therefore, one should focus on this phase to avoid polyploidization of cells and, thus, avoid polyploid plants.This study was supported in part by the Spanish seed company Semillas Fito S.A. (Barcelona, Spain) and IRTA, Institute of Agri-food Research and Technology, belonging to the Government of Catalonia, Spain.Peer reviewe

Agro-morphological diversity of some accessions of bread wheat (Triticuma estivum) in western Algeria

Abstract. Bellatreche A, Mahdad MY, Kaouadji Z, Gaouar SBS. 2017. Agro-morphological diversity of some accessions of breadwheat (Triticum aestivum) in western Algeria. Biodiversitas 18: 409-415. The diversity of wheat in Algeria is well known. An inventory has been conducted in the west of Algeria (North and South) to collect local accessions of bread wheat Triticuma aestivum for their morphological characterization. In this context, a collection of 23 traditional and new accessions of bread wheat was investigated using nine quantitative agro-morphological traits. The phenotypic diversity was determined by the Shannon-Weaver diversity index (H') at different levels (sample Totality which is by type of wheat and varietal name). The H' showed a wide phenotypic variability for different traits with H’ average of 0.59. The results of the multiple correspondence analysis and hierarchical clustering showed a clear distinction between different accessions. The results of this work have revealed the great phenotypic diversity of wheat accessions. These accessions were partially matched the names of varieties because of the existence of homonyms and synonyms in the names given by farmers. The results showed that these local accessions (that genetic composition should be studied more precisely) are mainly cultivated by traditional farmers who conserve this genetic resource, often on very small plots in the oases or in an inaccessible terrains. Keywords: Algeria, morphological diversity, morphological traits, Triticuma aestivu

Adventitious regeneration from haploid melon (Cucumis melo L.) leaves as an approach to increase the frequency of diploid plants

An efficient process of adventitious regeneration from haploid plant leaves to increase the proportion of diploid plants of six elite melon lines ‘Védrantais’ French cultivar, ‘Piel de Sapo’ Spanish line ‘T111’, and four near isogenic lines (NILs: SC 6–6, SC 7–2, SC 7–4, SC 8–4) has been developed. Several hormone combinations, in vitro culture conditions, and leaf positions on the plant stem were analyzed to improve the regeneration frequency and the percentage of diploid plants in these lines. Flow cytometry showed that diploid plant production was optimized by regeneration from haploid leaves as the percentage of diploid plants increased from 11% with regeneration of diploid cotyledons to 60% in this work. Evaluating the four NILs, only the lines SC 6–6 and SC 8–4 showed a significantly higher percentage of diploidy with 80% and 72.7%, respectively. The leaf position factor had no effect on regeneration and ploidy level, while darkness negatively influenced regeneration but had no effect on ploidy. Furthermore, it was concluded that the hormonal combination (cytokinin plus auxin) induces low endoreduplication activity, which is the phenomenon responsible for the polyploidy. Furthermore, this study provided evidence that in melon polyploidy occurs during the process of organogenesis, and, therefore, one should focus on this phase to avoid polyploidization of cells and, thus, avoid polyploid plants.info:eu-repo/semantics/acceptedVersio

Proposition of an appropriate technique to diagnose catheters fungal infectivities

Despite their great importance in the hospital environment, catheters are medical devices that can harm patients following their alterations by bacteria and/or fungal microorganisms. These alterations are classified into three types of infectivities, which may be simple contaminations/colonizations or serious infections. Bacterial infectivities were well studied using the technique of (Brun-Buisson et al., 1987); however, no technique was designed for yeast. Thus, we aimed to adapt the bacterial technique toward the assessment of the fungal infectivities. In order to check its reproducibility, both techniques were used in parallel. The results obtained were not consistent. That of Brun-Buisson et al. reflected more the catheters fungal contamination or colonization; nevertheless, the modified technique was better appropriate to the fungal infections of catheters considering the reduced time for its realization. Keywords: Fungal infectivities, Candida sp., Catheters, Diagnostic, Technica

Assessing the Wind Power Potential in Naama, Algeria to Complement Solar Energy through Integrated Modeling of the Wind Resource and Turbine Wind Performance

In the context of the escalating global climate crisis and the urgent need for sustainable energy solutions, this study explores the integration of wind energy as a supplementary source to solar photovoltaic energy in Naama, Algeria. The research utilizes a decade-long anemometric dataset, along with concurrent solar radiation data, to investigate the potential of harnessing wind energy, particularly during periods of low solar irradiance. Employing advanced statistical methods, including the Weibull distribution, the study assesses the wind power generation potential of a 2 kW/day turbine. The research highlights an average evening increase in wind speeds, which inversely correlates with the diminished solar energy production after sunset. This seasonal pattern is further substantiated by a significant negative correlation between wind speed and solar radiation for most of the year (January to May and September to December), with Pearson coefficients ranging from −0.713 to −0.524 (p < 0.05). However, the study also notes an absence of a notable correlation during the summer months (June to August) attributed to seasonal wind variations and the peak of solar irradiance. These findings confirm Naama as an ideal location for integrated renewable energy systems, thereby demonstrating the natural synergy between solar and wind energy. This synergy is particularly effective in mitigating the intermittency of solar power, thus highlighting the potential of wind energy during periods of low solar activity