19 research outputs found
Diffusion in sparse networks: linear to semi-linear crossover
We consider random networks whose dynamics is described by a rate equation,
with transition rates that form a symmetric matrix. The long time
evolution of the system is characterized by a diffusion coefficient . In one
dimension it is well known that can display an abrupt percolation-like
transition from diffusion () to sub-diffusion (D=0). A question arises
whether such a transition happens in higher dimensions. Numerically can be
evaluated using a resistor network calculation, or optionally it can be deduced
from the spectral properties of the system. Contrary to a recent expectation
that is based on a renormalization-group analysis, we deduce that is
finite; suggest an "effective-range-hopping" procedure to evaluate it; and
contrast the results with the linear estimate. The same approach is useful for
the analysis of networks that are described by quasi-one-dimensional sparse
banded matrices.Comment: 13 pages, 4 figures, proofed as publishe
CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, and less hands-on time. We implemented CEL-Seq2 on Fluidigm’s C1 system, providing its first single-cell, on-chip barcoding method, and we detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2’s increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use.Seventh Framework Programme (European Commission)Israel Science Foundatio
Enhancing spawning in the grey mullet (Mugil cephalus) by removal of dopaminergic inhibition
Inhibitory Control of the Brain-Pituitary Reproductive Axis of Male European Sea Bass: Role of Gonadotropin Inhibitory Hormone
Additional file 6: of CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
Supplementary file 2. CEL-Seq in C1. The file includes the complete information for performing CEL-Seq on the Fluidigm C1 instrument. (DOCX 17 kb
Additional file 1: Figure S1. of CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
Optimization of the CEL-Seq protocol. A Number of genes obtained from ten replicates of 100Â pg RNA performed with each type of primer: the original primer, the original primer with the inclusion of UMI, and the shortened UMI primer. B Number of transcripts identified for the two primers containing a UMI. C Estimating the efficiency of CEL-Seq using UMIs and ERCC spike-ins. The efficiency is computed as the y-intercept. D Side-by-side comparison of column clean-up, bead clean-up, and two RTs relative to CEL-Seq with a UMI primer. E Side-by-side comparison of different second-strand synthesis enzymes. The MessageAmp II enzyme was the one used originally. (PDF 519 kb
Central dopaminergic neurons in tilapia: Effects of gonadectomy and hypothalamic lesion
10.1016/0168-0102(94)90161-9Neuroscience Research184255-266NERA