Protective Effect of Tetrahydroxystilbene Glucoside on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway

Oxidative stress plays an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson's disease. The molecule, 2,3,5,4′-tetrahydr- oxystilbene-2-O-β-D-glucoside (TSG), is a potent antioxidant derived from the Chinese herb, Polygonum multiflorum Thunb. In this study, we investigated the protective effect of TSG against 6-hydroxydopamine-induced apoptosis in rat adrenal pheochromocytoma PC12 cells and the possible mechanisms. Our data demonstrated that TSG significantly reversed the 6-hydroxydopamine-induced decrease in cell viability, prevented 6-hydroxydopamine-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. In addition, TSG slowed the accumulation of intracellular reactive oxygen species and nitric oxide, counteracted the overexpression of inducible nitric oxide syntheses as well as neuronal nitric oxide syntheses, and also reduced the level of protein-bound 3-nitrotyrosine. These results demonstrate that the protective effects of TSG on rat adrenal pheochromocytoma PC12 cells are mediated, at least in part, by the ROS-NO pathway. Our results indicate that TSG may be effective in providing protection against neurodegenerative diseases associated with oxidative stress

Dystrophin hydrophobic regions in the pathogenesis of Duchenne and Becker muscular dystrophies

The aim of our study was to determine the role of dystrophin hydrophobic regions in the pathogenesis of Duchenne (DMD) and Becker (BMD) muscular dystrophies, by the Kyte-Doolittle scale mean hydrophobicity profile and 3D molecular models. A total of 1038 cases diagnosed with DMD or BMD with the in-frame mutation were collected in our hospital and the Leiden DMD information database in the period 2002-2013. Correlation between clinical types and genotypes were determined on the basis of these two sources. In addition, the Kyte-Doolittle scale mean hydrophobicity of dystrophin was analyzed using BioEdit software and the models of the hydrophobic domains of dystrophin were constructed. The presence of four hydrophobic regions is confirmed. They include the calponin homology CH2 domain on the actin-binding domain (ABD), spectrin-type repeat 16, hinge III and the EF Hand domain. The severe symptoms of DMD usually develop as a result of the mutational disruption in the hydrophobic regions I, II and IV of dystrophin – those that bind associated proteins of the dystrophin-glycoprotein complex (DGC). On the other hand, when the hydrophobic region III is deleted, the connection of the ordered repeat domains of the central rod domain remains intact, resulting in the less severe clinical presentation. We conclude that mutational changes in the structure of hydrophobic regions of dystrophin play an important role in the pathogenesis of DMD

Intercomparison of Solid Precipitation Derived from the Weighting Rain Gauge and Optical Instruments in the Interior Qinghai-Tibetan Plateau

Due to the light precipitation and strong wind in the cold season, it is hard to get credible solid precipitation on the Qinghai-Tibetan plateau (QTP). To address this issue, two kinds of optical instruments, the Thies Laser Precipitation Monitor (LPM) and OTT laser-optical Particle Size Velocity (Parsivel), were used on QTP. The measured precipitation was compared with the precipitation derived from Geonor T-200B precipitation gauge (Geonor). The results showed that Geonor was hard to catch light precipitation (precipitation amount was less than 1 mm during a single event) when the wind speeds were higher than 3.5 m/s. Even when the wind speeds were smaller than 3.5 m/s, about 44% of such light precipitation events were not recorded by Geonor. The optical instruments had much better performance in recording light precipitation. Three methods were used to correct Geonor measurements of daily solid precipitation and the corrected values were set as reference for assessing the performance of LPM and Parsivel; the results showed that LPM had good performance in measuring the solid precipitation but Parsivel overestimated the precipitation amount. Methods for correcting Geonor’s hourly solid precipitation and recalculating Parsivel’s solid precipitation amount were also proposed in this paper

Evaluating the Impact of Soil Enthalpy upon the Thawing Process of the Active Layer in Permafrost Regions of the Qinghai–Tibet Plateau Using CLM5.0

The hydrothermal dynamics of the active layer is a key issue in the study of surface processes in permafrost regions. Even though the soil energy budget is controlled by thermal conduction and latent heat transfer, few studies have focused on their effects upon the active layer thickness (ALT). In the present study, the community land model (CLM) version 5.0 is used to simulate the soil temperature and moisture of the active layers at the Tanggula (TGL) and Beiluhe (BLH) stations in permafrost regions of the Qinghai–Tibet Plateau based on the theory of soil enthalpy in order to estimate the soil energy state and analyze the energy changes in the active layer during freezing and thawing. The results indicate that the soil enthalpy has significant seasonal variation characteristics, which accurately reflected the freezing and thawing processes of the active layer. The change in soil enthalpy is significantly related to the thawing depth of the active layer in TGL and BLH, and its changing process can be expressed as an exponential relationship. Near the surface, the variation of the energy due to temperature gradient and actual evaporation can also be expressed as an exponential relationship. The promoting effect of heat conduction on the ALT is greater than the inhibiting effect of latent heat transfer, with the energy contribution from the phase change accounting for about 20–40% of the energy due to the temperature gradient. The thawing depth increases by 14.16–18.62 cm as the energy due to the temperature gradient increases by 1 MJ/m2 and decreases by 2.75–7.16 cm as the energy due to the phase change increases by 1 MJ/m2. Thus, the present study quantifies the effects of soil energy upon the ALT and facilitates an understanding of the hydrothermal processes in soils in permafrost regions

Spatiotemporal Patterns and Regional Differences in Soil Thermal Conductivity on the Qinghai–Tibet Plateau

The Qinghai–Tibet Plateau is an area known to be sensitive to global climate change, and the problems caused by permafrost degradation in the context of climate warming potentially have far-reaching effects on regional hydrogeological processes, ecosystem functions, and engineering safety. Soil thermal conductivity (STC) is a key input parameter for temperature and surface energy simulations of the permafrost active layer. Therefore, understanding the spatial distribution patterns and variation characteristics of STC is important for accurate simulation and future predictions of permafrost on the Qinghai–Tibet Plateau. However, no systematic research has been conducted on this topic. In this study, based on a dataset of 2972 STC measurements, we simulated the spatial distribution patterns and spatiotemporal variation of STC in the shallow layer (5 cm) of the Qinghai–Tibet Plateau and the permafrost area using a machine learning model. The monthly analysis results showed that the STC was high from May to August and low from January to April and from September to December. In addition, the mean STC in the permafrost region of the Qinghai–Tibet Plateau was higher during the thawing period than during the freezing period, while the STC in the eastern and southeastern regions is generally higher than that in the western and northwestern regions. From 2005 to 2018, the difference between the STC in the permafrost region during the thawing and freezing periods gradually decreased, with a slight difference in the western hinterland region and a large difference in the eastern region. In areas with specific landforms such as basins and mountainous areas, the changes in the STC during the thawing and freezing periods were different or even opposite. The STC of alpine meadow was found to be most sensitive to the changes during the thawing and freezing periods within the permafrost zone, while the STC for bare land, alpine desert, and alpine swamp meadow decreased overall between 2005 and 2018. The results of this study provide important baseline data for the subsequent analysis and simulation of the permafrost on the Qinghai–Tibet Plateau

Retrieving Soil Moisture in the Permafrost Environment by Sentinel-1/2 Temporal Data on the Qinghai–Tibet Plateau

Soil moisture (SM) products presently available in permafrost regions, especially on the Qinghai–Tibet Plateau (QTP), hardly meet the demands of evaluating and modeling climatic, hydrological, and ecological processes, due to their significant bias and low spatial resolution. This study developed an algorithm to generate high-spatial-resolution SM during the thawing season using Sentinel-1 (S1) and Sentinel-2 (S2) temporal data in the permafrost environment. This algorithm utilizes the seasonal backscatter differences to reduce the effect of surface roughness and uses the normalized difference vegetation index (NDVI) and the normalized difference moisture index (NDMI) to characterize vegetation contribution. Then, the SM map with a grid spacing of 50 m × 50 m in the hinterland of the QTP with an area of 505 km × 246 km was generated. The results were independently validated based on in situ data from active layer monitoring sites. It shows that this algorithm can retrieve SM well in the study area. The coefficient of determination (R2) and root-mean-square error (RMSE) are 0.82 and 0.06 m3/m3, respectively. This study analyzed the SM distribution of different vegetation types: the alpine swamp meadow had the largest SM of 0.26 m3/m3, followed by the alpine meadow (0.23), alpine steppe (0.2), and alpine desert (0.16), taking the Tuotuo River basin as an example. We also found a significantly negative correlation between the coefficient of variation (CV) and SM in the permafrost area, and the variability of SM is higher in drier environments and lower in wetter environments. The comparison with ERA5-Land, GLDAS, and ESA CCI showed that the proposed method can provide more spatial details and achieve better performance in permafrost areas on QTP. The results also indicated that the developed algorithm has the potential to be applied in the entire permafrost regions on the QTP

A first assessment of satellite and reanalysis estimates of surface and root-zone soil moisture over the permafrost region of Qinghai-Tibet Plateau

International audienceLong-term and high-quality surface soil moisture (SSM) and root-zone soil moisture (RZSM) data is crucial for understanding the land-atmosphere interactions of the Qinghai-Tibet Plateau (QTP). More than 40% of QTP is covered by permafrost, yet few studies have evaluated the accuracy of SSM and RZSM products derived from microwave satellite, land surface models (LSMs) and reanalysis over that region. This study tries to address this gap by evaluating a range of satellite and reanalysis estimates of SSM and RZSM in the thawed soil overlaying permafrost in the QTP, using in-situ measurements from sixteen stations. Here, seven SSM products were evaluated: Soil Moisture Active Passive L3 (SMAP L3) and L4 (SMAP-L4), Soil Moisture and Ocean Salinity in version IC (SMOS IC), Land Parameter Retrieval Model (LPRM) Advanced Microwave Scanning Radiometer 2 (AMSR2), European Space Agency Climate Change Initiative (ESA CCI), Advanced Scatterometer (ASCAT), and the fifth generation of the land component of the European Centre for Medium-Range Weather Forecasts atmospheric reanalysis (ERAS-Land). We also evaluated three RZSM products from SMAP-L4, ERA5-Land, and the Noah land surface model driven by Global Land Data Assimilation System (GLDAS-Noah). The assessment was conducted using five statistical metrics, i. e. Pearson correlation coefficient (R), bias, slope, Root Mean Square Error (RMSE), and unbiased RMSE (ubRMSE) between SSM or RZSM products and in-situ measurements. Our results showed that the ESA CCI, SMAP-L4 and SMOS-IC SSM products outperformed the other SSM products, indicated by higher correlation coefficients (R) (with a median R value of 0.63, 0.44 and 0.57, respectively) and lower ubRMSE (with a median ubRMSE value of 0.05, 0.04 and 0.07 m(3)/m(3), respectively). Yet, SSM overestimation was found for all SSM products. This could be partly attributed to ancillary data used in the retrieval (e.g. overestimation of land surface temperature for SMAP-L3) and to the fact that the products (e.g. LPRM) more easily overestimate the in-situ SSM when the soil is very dry. As expected, SMAP-L3 SSM performed better in areas with sparse vegetation than with dense vegetation covers. For RZSM products, SMAP-L4 and GLDAS-Noah (R = 0.66 and 0.44, ubRMSE = 0.03 and 0.02 m(3)/m(3), respectively) performed better than ERAS-Land (R = 0.46; ubRMSE = 0.03 m(3)/m(3)). It is also found that all RZSM products were unable to capture the variations of in-situ RZSM during the freezing/thawing period over the permafrost regions of QTP, due to large deviation for the ice-water phase change simulation and the lack of consideration for unfrozen-water migration during freezing processes in the LSMs

JinqiJiangtang tablets for pre-diabetes: A randomized, double-blind and placebo-controlled clinical trial

Abstract This study observed the efficacy and safety of JinqiJiangtang tablets (JQJT tablets, a traditional Chinese patent medicine) for pre-diabetes. Four hundred patients with pre-diabetes at five centres were treated for 12months and followed for an additional 12months to investigate the preventative effects of JQJT tablets (Registration ID: ChiCTR-PRC-09000401). The incidence rate of diabetes mellitus was the primary endpoint. The risk of converting from pre-diabetes to diabetes was 0.58-fold less in the JQJT tablets group than in the placebo group [HR (95% CI): 0.58 (0.384, 0.876), P = 0.010]. Furthermore, the probability of achieving normalized blood glucose was 1.41-fold greater in the JQJT tablets group than in the placebo group [HR (95% CI): 1.41 (1.002, 1.996), P = 0.0049]. ITT analysis revealed that the incidence of diabetes upon treatment completion was 16.5% in the JQJT tablets group compared with 28.9% in the control group. The percentage of patients with normalized blood glucose upon 12-month intervention was 41.8% in the JQJT tablets group compared with 27.8% in the control group. JQJT tablets could be an effective intervention for preventative treatment of Type 2 diabetes mellitus