Identification of miRNAs and their targets by high-throughput sequencing and degradome analysis in cytoplasmic male-sterile line NJCMS1A and its maintainer NJCMS1B of soybean

Table S1. Summary of small RNA annotations from NJCMS1A and NJCMS1B. Table S2. Known miRNAs identified in NJCMS1A and NJCMS1B. Table S3. Family member distribution in conserved miRNA families. Table S4. Summary of miRNA families found in NJCMS1A and NJCMS1B. Table S5. Novel miRNAs on the other arm of known pre-miRNAs. Table S6. Novel miRNAs identified in NJCMS1A and NJCMS1B. Table S7-1. High-confidence known miRNAs identified in NJCMS1A and NJCMS1B. Table S7-2. High-confidence novel miRNAs identified in NJCMS1A and NJCMS1B. Table S8-1. The up-regulated miRNAs identified in NJCMS1A and NJCMS1B. Table S8-2. The down-regulated miRNAs identified in NJCMS1A and NJCMS1B. Table S9. The targets of miRNAs identified in NJCMS1A and NJCMS1B. Table S10. Targets of novel miRNAs in NJCMS1A and NJCMS1B. Table S11. Primers used in this study. (ZIP 637 kb

Superconductivity induced by doping Ru in SrFe2-xRuxAs2

Using one-step solid state reaction method, we have successfully synthesized the superconductor SrFe1-xRuxAs. X-ray diffraction indicates that the material has formed the ThCr2Si2-type structure with a space group I4/mmm. The systematic evolution of the lattice constants demonstrates that the Fe ions are successfully replaced by the Ru. By increasing the doping content of Ru, the spin-density-wave (SDW) transition in the parent compound is suppressed and superconductivity emerges. The maximum superconducting transition temperature is found at 13.5 K with the doping level of x = 0.7. The temperature dependence of DC magnetization confirms superconducting transitions at around 12 K. Our results indicate that similar to non-isoelectronic substitution, isoelectronic substitution contributes to changes in both the carrier concentration and internal pressure, and superconductivity could be induced by isoelectronic substitution.Comment: 14 pages, 4 figure

Association between a body shape index and cognitive impairment among US older adults aged 40 years and above from a cross-sectional survey of the NHANES 2011-2014

PurposeThis research aimed to assess the correlation between the Adjusted Body Shape Index (ABSI) and the presence of abdominal aortic calcification (AAC) among middle-aged and older American adults.MethodsEmploying a cross-sectional design, this study analyzed data from the 2013-2014 National Health and Nutrition Examination Survey (NHANES), focusing on 3077 participants aged 40 and above. AAC detection was conducted using dual-energy X-ray absorptiometry (DXA). ABSI was determined based on waist circumference (WC), weight, and height data. The association between ABSI and AAC was examined through multiple linear regression, smoothed curve analysis, threshold effect evaluation, subgroup analysis, and interaction testing.ResultsThe study encompassed 3077 individuals aged 40 and above. Findings indicated a noteworthy positive relationship between ABSI and AAC when adjusting various covariates. Analysis of threshold effects identified a K-point at 0.0908, showing no significant effect to its left but a significant effect to its right. Further, subgroup and interaction analyses highlighted the ABSI-AAC connection specifically within different age groups and among individuals with diabetes.ConclusionHigher ABSI was correlated with higher AAC score

Natural Convection of Cu-Gallium Nanofluid in Enclosures

In this work, the natural convection heat transfer of Cu-gallium nanofluid in a differentially heated enclosure is investigated. A single-phase model is employed with constant or temperature-dependent properties of the fluid. The results are shown over a wide range of Grashof numbers, volume fractions of nanoparticles, and aspect ratios. The Nusselt number is demonstrated to be sensitive to the aspect ratio. It is found that the Nusselt number is more sensitive to thermal conductivity than viscosity at a low velocity (especially for a low aspect ratio and a low Grashof number), however, it is more sensitive to the viscosity than the thermal conductivity at a high velocity (high aspect ratio and high Grashof number). In addition, the evolution of velocity vectors, isotherms, and Nusselt number for a small aspect ratio is investigated

The EGFR tyrosine kinase inhibitors as second-line therapy for EGFR wild-type non-small-cell lung cancer: a real-world study in People's Republic of China

INTRODUCTION: Clinical evidence comparing chemotherapy and tyrosine kinase inhibitors (TKIs) as second-line therapy for epidermal growth factor receptor (EGFR) wild-type non-small-cell lung cancer (NSCLC) are conflicting. METHODS: We retrospectively reviewed stage IV EGFR wild-type NSCLC patients who relapsed on first-line chemotherapy at the Shanghai Chest Hospital to compare the efficacy of TKIs and chemotherapy as second-line therapy among different clinical subgroups. RESULTS: The progression-free survival (PFS) and overall survival for patients receiving chemotherapy as second-line therapy for NSCLC were longer than patients who received TKIs. The hazard ratios (HRs) were 0.40 (P<0.001) and 0.50 (P<0.001), respectively. Subgroup analyses showed that second-line TKI therapy resulted in inferior PFS among smokers (HR =0.24, P<0.001), males (HR =0.33, P<0.001), females (HR =0.54, P=0.004), and patients with adenocarcinoma (HR =0.48, P<0.001) and nonadenocarcinoma histology (HR =0.20, P<0.001). Among never-smokers, the PFS in cohorts receiving second-line chemotherapy or TKIs was not significantly different (HR =0.70, P=0.08). CONCLUSION: These results suggest that EGFR TKI therapy was inferior compared to chemotherapy in EGFR wild-type NSCLC patients who relapsed from first-line chemotherapy; however, among never-smokers, these two treatment strategies were comparable

Object-Centric Multiple Object Tracking

Unsupervised object-centric learning methods allow the partitioning of scenes into entities without additional localization information and are excellent candidates for reducing the annotation burden of multiple-object tracking (MOT) pipelines. Unfortunately, they lack two key properties: objects are often split into parts and are not consistently tracked over time. In fact, state-of-the-art models achieve pixel-level accuracy and temporal consistency by relying on supervised object detection with additional ID labels for the association through time. This paper proposes a video object-centric model for MOT. It consists of an index-merge module that adapts the object-centric slots into detection outputs and an object memory module that builds complete object prototypes to handle occlusions. Benefited from object-centric learning, we only require sparse detection labels (0%-6.25%) for object localization and feature binding. Relying on our self-supervised Expectation-Maximization-inspired loss for object association, our approach requires no ID labels. Our experiments significantly narrow the gap between the existing object-centric model and the fully supervised state-of-the-art and outperform several unsupervised trackers.Comment: ICCV 2023 camera-ready versio

DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.

BACKGROUND(#br)Multiple morphological abnormalities of the sperm flagella (MMAF) is one kind of severe asthenozoospermia, which is caused by dysplastic development of sperm flagella. In our study, we sought to investigate the novel gene mutations leading to severe asthenozoospermia and MMAF.(#br)METHODS AND MATERIALS(#br)The patient’s spermatozoa were tested by Papanicolaou staining and transmission electron microscopy. Whole exome sequencing was performed on the patient with severe asthenozoospermia and MMAF. Sanger sequencing verified the mutations in the family. The expression of DNAH17 was detected by immunofluorescence and Western blot.(#br)RESULTS(#br)Spermatozoa sample from the patient showed severe asthenozoospermia and MMAF. We detected biallelic mutations (c.C4445T, p.A1482V and c.C6857T, and p.S2286L) in DNAH17 (MIM:610063). The protein expression of DNAH17 was almost undetectable in spermatozoa from the patient with the biallelic mutations.(#br)CONCLUSION(#br)These results demonstrated that DNAH17 may be involved in severe asthenozoospermia and MMAF