Dynamic estimating the karst tunnel water inrush based on monitoring data during excavation

The tunnel water gushing has long been a difficult hydrogeological problem, especially in karst areas. It affects the entire process of tunnel construction, operation and maintenance. In view of the complex disaster-causing mechanism and difficult quantitative predictions of water inrush, several theoretical methods are adopted to realize dynamic assessment of water inrush in the progressive process of tunnel construction. According to a survey conducted in the Zoumaling tunnel near Chongqing, China, 62% of its total length, e.g., 1525 m is associated with karst（including a fault fracture zone). On the basis of collecting real-time monitoring data about water inrush in the excavated section of the Zoumaling tunnel, a fuzzy data analysis method has been used to analyze the content of seven common ions in the inflow water, which makes it possible to classify the groundwater types and to establish the hydrogeological model of the tunnel site. In order to forecast the possibility and quantity of water inrush, it is essential to accurately model the groundwater system spatially. The preliminary forecasting result about untapped section reveals a small possibility of a sudden water inflow disaster and 35,000 m3/d water inflow, which is close to the ultimately measured quantity of water. This study provides a theoretical reference for the prediction of water inrush during tunnel construction, and the main characteristic of this study is reflected in the real-time prediction of tunnel water inrush according to actual tunnel inflow of excavated sections. This approach can be applied in similar situations for the prediction of tunnel water inrush in other karst regions.Key words: karst region, tunnel water inrush; dynamic estimate; fuzzy cluster analysis.Pričakovana dinamika vdora vode v predore na podlagi meritev med njihovo gradnjoPojav vdiranja vode v predore je že dolgo časa poznana težava, še posebej na kraških območjih. Pojavlja se med celotno gradnjo predorov, njihovo uporabo in vzdrževanjem. Za proučevanje potencialnega pojava nesreč in težavnega napovedovanja količine vdora vode je bilo preizkušenih več različnih teoretičnih metod. Te omogočajo oceno dinamike vdora vode med celotnim procesom gradnje predorov. Pri predoru Zoumaling v bližini mesta Chongqing (Kitajska) približno 62 % dolžine predora (1525 m) poteka na območju krasa in čez prelomna območja. Na podlagi v realnem času zbranih podatkov o vdorih vode v izkopanih odsekih predora Zoumaling se je naredila analiza mehkih množic. Ta je bila uporabljena za analizo sedmih v vodi najbolj značilnih ionov in je omogočila razvrstitev podzemne vode v različne skupine, s tem pa izdelavo hidrogeološkega modela neposredne okolice predora. Za analizo verjetnosti vdora vode in njene možne količine je izdelava natančnega modela vodonosnika zelo pomembna. Prvi rezultati, ki se nanašajo na en še nedokončan odsek, kažejo na majhno možnost nenadnega vdora vode. Največja možna dnevna količina vdora je ocenjena na 35.000 m3, kar je blizu najvišje izmerjene dnevne količine dotoka. Pričujoča raziskava vzpostavlja teoretično podlago za napoved vdora vode v času gradnje predora, glavna posebnost pa je napoved vdora v realnem času na podlagi izmerjenega dotoka v že izkopanih odsekih predora. Predstavljen postopek in napovedi, ki jih omogoča, se lahko uporabijo v podobnih primerih tudi na drugih kraških območjih.Ključne besede: kraško območje, vdor vode v predore, pričakovana dinamika, analiza mehkih množic.

Association of LMP/TAP Gene Polymorphisms with Tuberculosis Susceptibility in Li Population in China

Background: Tuberculosis (TB) is a contagious disease affected by multiple genetic and environmental factors. Several association studies have suggested that cellular immune response is vital for controlling and preventing of tuberculosis infection. Low molecular weight polypeptides (LMPs) and transporters with antigen processing (TAPs) are the main molecules in the processing and presentation pathway for intracellular antigens. This study was performed to elucidate whether these antigen-processing genes (LMP/TAP) polymorphisms could be associated with the risk of tuberculosis infection in China. Methodology/Principal Findings: We recruited 205 active pulmonary tuberculosis patients and 217 normal controls from Li population for this study. Four polymorphisms of LMP/TAP genes were determined by PCR-RFLP assay and haplotypes were constructed by software PHASE 1.0. Of the total four polymorphisms, genotype frequencies of LMP7 AA homozygote and CA heterozygote were significantly greater among cases compared to controls, with odds ratio of 3.77 (95 % CI: 1.60–8.89; P = 0.002) and 2.97 (95 % CI: 1.80–4.90; P,0.0001), respectively. The genotypes of TAP1-2 GG homozygote and AG heterozygote were more frequent in subjects with TB than in controls, with odds ratio of 3.94 (95 % CI: 1.82–8.53; P = 0.001) and 2.87 (95 % CI: 1.75–4.71; P,0.0001), respectively. Similarly, we found that haplotype B which carried LMP7 and TAP1-2 variations significantly increased the susceptibility to TB (OR = 3.674, 95 % CI: 2.254–5.988; P,0.0001). Moreover, it i

Hydrochemical characteristics and genesis analysis of typical aquifer system in karst critical zone of Central Yunnan Plateau

The study of hydrogeochemical processes in the karst critical zone (KCZ) is of great significance for the scientific understanding of their internal evolutionary environment and structural characteristics. Karst groundwater is the main information carrier after water-rock interactions. Quantitative analysis of its hydrochemical characteristics and causes is an effective means to reveal the medium environment and hydrodynamic conditions of the aquifer system in the KCZ. In this paper, three typical karst aquifer systems in the KCZ of the central Yunnan Plateau were taken as the research objects. Through field sampling and laboratory testing of karst springs exposed by different aquifer systems, mathematical statistics analysis, hydrochemical diagram, ion ratio coefficient and hydrogeochemical simulation were comprehensively used to deeply analyze the characteristics of hydrochemical components, genesis and aquifer medium of karst groundwater in each aquifer system; the internal relationship and law between the water cycle and hydrochemistry in the key belt were discussed. The results showed that: ①HCO3- and Ca2+ were the highest and most stable ion components in regional karst groundwater, and Mg2+ was the key factor controlling the alienation of hydrochemical types in each aquifer system; ②The rock weathering and mineral dissolution of carbonate rocks were the main causes of the chemical composition characteristics of karst water in each aquifer system, and karst groundwater dissolution on the aquifer of the Huaning aquifer system was still occurring. The alternation of cation adsorption and the weathering and dissolution of silicate rocks were the main sources of Na+ and K+ in regional karst groundwater; ③The development intensity of regional karst, the exposed condition of karst aquifers and the lithology and connectivity of aquifer media jointly shaped the groundwater chemical characteristics of different aquifer systems in the KCZ of the Central Yunnan Plateau

Profiling the Bacterial Diversity in a Typical Karst Tiankeng of China

While karst tiankengs have a higher capacity to act as safe havens for biodiversity in changing climates, little is known about their soil microorganisms. To fill this gap, we investigate the distribution and driving factors of the bacterial community in karst tiankeng systems. There is a significant difference in the soil characteristics between the inside and the outside of a karst tiankeng. At the karst tiankeng considered in this study, the bacterial composition, in terms of the operational taxonomic unit (OTU), was found to be significantly different in different soil samples, taken from diverse sampling sites within the collapsed doline or the external area, and showed a high habitat heterogeneity. The dominant phylum abundances vary with the sampling sites and have their own indicator taxa from phylum to genus. Unlike the primary controlling factors of plant diversity, the microclimate (soil moisture and temperature), soil pH, and slope dominated the distribution of the bacterial community in karst tiankeng systems. Our results firstly showed the distribution characteristics of bacterial communities and then revealed the importance of microhabitats in predicting the microbial distribution in karst tiankeng systems

Demographic characteristics for tuberculosis cases and controls.

<p>Demographic characteristics for tuberculosis cases and controls.</p

Genotype, allele frequencies of the polymorphisms in the LMP and TAP genes.

<p>Significant p values are in bold.</p>*<p>Logistic regression model, adjusted by gender, age, smoke and drink.</p

Distribution of haplotype frequencies in LMP/TAP genes among cases and controls.

a<p>Logistic regression model, adjusted by gender, age, smoke and drink.</p>b<p>– denotes any haplotype, for example: –/A indicates the A haplotype in combination with any other haplotype.</p

Haplotypes frequencies of the LMP/TAP genes in cases and controls.

<p>Haplotypes frequencies of the LMP/TAP genes in cases and controls.</p

Primer sets used for amplification and sequencing of LMP/TAP gene.

<p>Primer sets used for amplification and sequencing of LMP/TAP gene.</p

Genotype, allele frequencies and sex specific association analysis.

<p>Significant p values are in bold.</p>*<p>Logistic regression model, adjusted by gender, age, smoke and drink.</p