Les produits cosmétiques naturels et biologiques

BESANCON-BU Médecine pharmacie (250562102) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Pénétration cutanée de liquides volatils toxiques

BESANCON-BU Médecine pharmacie (250562102) / SudocSudocFranceF

Optimisation et transposition de la granulation humide et du séchage en cuve

BESANCON-BU Médecine pharmacie (250562102) / SudocSudocFranceF

Formulation de liposomes d'interleukine-2 conventionnels ou à temps de circulation prolongé et spécifiques des cellules du système immunitaire

L'Interleukine-2 (IL-2) joue un rôle clef dans la réponse immunitaire ce qui lui confère de nombreux intérêts dans le traitement des cancers et du VIH. La demi-vie très courte de l'IL-2 après administration intraveineuse nécessite la mise en place de protocoles thérapeutiques lourds à l'origine d'effets secondaires importants. Parmi les stratégies envisagées pour diminuer ces effets indésirables, nous avons choisi de formuler des liposomes d'IL-2. Une méthode de dosage par fluorescence de l'IL-2 associée aux liposomes a tout d'abord été mise au point. Les effets, sur l'activité biologique', de l'IL-2, des conditions de formulation des liposomes et des interactions protéine-lipide ont été étudiées. L'objectif final de cette thèse a été de formuler des liposomes à temps de rémanence accrue et spécifiques des cellules du système immunitaire. Ces vecteurs permettraient d'allonger la demie vie de la protéine et de la libérer au contact des cellules cibles améliorant ainsi son efficacitéThe key role played by the cytokine Interleukin-2 (IL-2) in the immune response gives it great potential for the treatment of cancers and HW infection. However, the short haif-life ofIL-2 after intravenous administration means that large doses have to be given leading to serious side-effects. We have formulated liposomes containing IL-2 as one strategy for reducing these side-effects. We first developed a method based on the intrinsic fluorescence of IL-2 to measure its association with liposomes. The effect of the preparation conditions of liposomes on the biological activity of IL-2 and the nature of the lipid-protein interactions were studied. The ultimate aim of this work was to formulate long-circulating liposomes which were specifically targeted to cells of the immune system. This carrier system could increase the circulating haif-life of the protein and release it in the vicinity of the target cells, thus increasing its efficacy.CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Bonnes pratiques de fabrication des médicaments vétérinaires en recherche et développement

BESANCON-BU Médecine pharmacie (250562102) / SudocSudocFranceF

Adaptation de l'offre cosmétique anti-âge aux tendances actuelles (la médicalisation des soins cosmétiques)

BESANCON-BU Médecine pharmacie (250562102) / SudocSudocFranceF

Dosage par spectrophotométrie proche infrarouge et amélioration du procédé de fabrication du Polykaraya® (Sanofi)

BESANCON-BU Médecine pharmacie (250562102) / SudocSudocFranceF

Bioadhesive pellets increase local 5-aminosalicylic acid concentration in experimental colitis

Topical delivery of 5-aminosalicylic acid (5-ASA) to the colonic mucosa is important in order to achieve effective drug concentration in the site of inflammation and to minimize its systemic availability. 5-ASA loaded pellets were prepared by an extrusion/spheronization method. Mucoadhesive biopolymer chitosan was incorporated into the pellets, and drug delivery to the colon was controlled by the pH-sensitive polymer Eudragit® FS. Dissolution profiles of coated pellets revealed no drug release at pH 1.2 within 2h and release as intended in the simulated distal ileum and colon. In vivo, chitosan-core drug loaded pellets (AMCh) showed 2.5-fold higher drug metabolite concentration than after chitosan free pellets (AM) administration in the inflamed colonic tissue. Additionally, AMCh demonstrated decreased in AUC in colitis group (1507 ± 400 ng h/ml) compared with AM (1907 ± 122 ng h/ml). In terms of therapeutic efficiency, administration of pellets markedly decreased the colon/body weight ratio (colitis: 0.0355 ± 0.0028; AM 0.0092 ± 0.0033; AMCh 0.0086 ± 0.0022) and myeloperoxidase activity (colitis: 3212 ± 294 U/g tissue; AM 796 ± 211 U/g; AMCh 552 ± 319 U/g). Bioadhesive chitosan pellets showed additional beneficial properties for colonic 5-ASA delivery in the treatment of inflammatory bowel disease by increasing the drug concentration locally

Small silica nanoparticles transiently modulate the intestinal permeability by actin cytoskeleton disruption in both Caco-2 and Caco-2/HT29-MTX models

International audienc

Investigation of the spontaneous nanoemulsification process with medium- and long-chain triglycerides

International audienceOil-in-water nanoemulsions are used in numerous biomedical applications as delivery systems. The droplet size in the nanometer range and their composition were extensively developed for carrying and enhancing the absorption of lipophilic drugs and lipids of interest. In the present study, critical parameters involved in the spontaneous nanoemulsification process such as the temperature, the oil type, the surfactant-to-oil and water-to-oil ratios were investigated. The aim was to design a solvent-free procedure for the spontaneous nanoemulsification at a low temperature of a large variety of triglycerides including vegetable oils. Nanoemulsification of medium-chain triglyceride (MCT) was not dependent on the temperature while nanodroplets of long-chain triglycerides (LCT) were only obtained by reaching the cloud point of ethoxylated surfactant Kolliphor® HS15. The molar volume of triglycerides was considered as a predictive parameter governing both, the spontaneous nanoemulsification at low temperature and the Ostwald ripening rate. The physical mixture of MCT and LCT was a promising strategy to prepare stable and fine nanoemulsions at 37 °C. They were characterized by a hydrodynamic diameter comprised between 20 and 30 nm and a narrow size distribution. These findings pave the way to new applications for the parenteral nutrition and the delivery of thermosensitive drugs and lipophilic molecules such as antioxidants