A Study on Project-Driven Practice and Innovation Abilities of IT Students’ Club

IT students’ clubs play an irreplaceable role in training students’ innovation and practical abilities. Through questionnaires and interviews, the paper analyzes students’ participation in IT clubs, the training of students’ innovation and practical abilities and the problems existing in the organization management of clubs. By referring to the successful experience of Wuhan University of Science and Technology in the practice teaching reform, a “project-driven” system for training practice and innovation abilities, which focused on training students’ innovation spirit and practical abilities, was established and achieved remarkable success

An Innovative Practice System of Information Management and Information System Specialty Based on Actual Projects

The practical teaching is the key link of training the students’ practical and creative ability in major of information management and information system. On the basis of analyzing the training goal and social demand of the major, an innovative practice system based on actual projects is constructed to solve the main problems exist in the practical teaching, by combining with the successful experience of the practical teaching innovation in Wuhan University of Science and Technology. The system provides a good practical teaching environment and an innovation practical platform for the cultivation of high quality innovation talents. To study in actual projects will accumulate the students’ project experience, cultivate their innovation consciousness and promote their competition ability

The Neuronal Channel NALCN Contributes Resting Sodium Permeability and Is Required for Normal Respiratory Rhythm

SummarySodium plays a key role in determining the basal excitability of the nervous systems through the resting “leak” Na+ permeabilities, but the molecular identities of the TTX- and Cs+-resistant Na+ leak conductance are totally unknown. Here we show that this conductance is formed by the protein NALCN, a substantially uncharacterized member of the sodium/calcium channel family. Unlike any of the other 20 family members, NALCN forms a voltage-independent, nonselective cation channel. NALCN mutant mice have a severely disrupted respiratory rhythm and die within 24 hours of birth. Brain stem-spinal cord recordings reveal reduced neuronal firing. The TTX- and Cs+-resistant background Na+ leak current is absent in the mutant hippocampal neurons. The resting membrane potentials of the mutant neurons are relatively insensitive to changes in extracellular Na+ concentration. Thus, NALCN, a nonselective cation channel, forms the background Na+ leak conductance and controls neuronal excitability

Integrative analysis of grapevine (Vitis vinifera L) transcriptome reveals regulatory network for Chardonnay quality formation

Anthocyanins, total phenols, soluble sugar and fruit shape plays a significant role in determining the distinct fruit quality and customer preference. However, for the majority of fruit species, little is known about the transcriptomics and underlying regulatory networks that control the generation of overall quality during fruit growth and ripening. This study incorporated the quality-related transcriptome data from 6 ecological zones across 3 fruit development and maturity phases of Chardonnay cultivars. With the help of this dataset, we were able to build a complex regulatory network that may be used to identify important structural genes and transcription factors that control the anthocyanins, total phenols, soluble sugars and fruit shape in grapes. Overall, our findings set the groundwork to improve grape quality in addition to offering novel views on quality control during grape development and ripening

A non-dispersive infrared sensor for real-time detection of cyanogen chloride

Cyanogen chloride, as a systemic toxic agent, can cause death rapidly. In this paper, a non-dispersive infrared sensor was designed for the infrared  absorption detection of cyanogen chloride at 800 cm−1. The roughness of the internal coating material was analyzed by experiments, and the gold-  plated gas chamber was selected. The light path propagation of different cross-section gas chambers was simulated, and the circular section gas  chamber was selected to increase the infrared detector signal. The effect of flow rate on voltage was studied. The standard curve between voltage and  concentration was obtained under the optimal condition of 0.4 L min−1. The maximum response time was 19 s, and RSD was less than 2%. The  interference experiment results showed that common gases entering the gas chamber do not cause interference. The non-dispersive infrared sensor for  cyanogen chloride has good stability and detects cyanogen chloride in real-time

Metabolism of androstenone, 17β-estradiol and dihydrotestosterone in primary cultured pig hepatocytes and the role of 3β-hydroxysteroid dehydrogenase in this process

© 2015 Chen et al. Steroids metabolism plays an important role in mammals and contributes to quality of pig meat. The main objective of this study was to identify metabolites of androstenone, 17β-estradiol and dihydrotestosterone using primary cultured pig hepatocytes as a model system. The role of 3β-hydroxysteroid dehydrogenase (3βHSD) in regulation of steroid metabolism was also validated using trilostane, a specific 3βHSD inhibitor. Steroid glucuronide conjugated metabolites were detected by liquid chromatography time of flight mass spectrometry (LC-TOF-MS). 3βHSD enzyme was essential for metabolism of androstenone to 5α-androst-16-en-3β-ol, which then formed androstenone glucuronide conjugate. Metabolism of 17β-estradiol was accompanied by formation of glucuronide-conjugated estrone and glucuronide-conjugated estradiol. The ratio of the two metabolites was around 5:1. 3βHSD enzyme was not involved in 17β-estradiol metabolism. 5α-Dihydrotestosterone-17β-glucuronide was identified as a dihydrotestosterone metabolite, and this metabolism was related to 3βHSD enzyme activity as demonstrated by inhibition study

Transcriptome analysis of the hepatopancreas from the Litopenaeus vannamei infected with different flagellum types of Vibrio alginolyticus strains

Vibrio alginolyticus, one of the prevalently harmful Vibrio species found in the ocean, causes significant economic damage in the shrimp farming industry. Its flagellum serves as a crucial virulence factor in the invasion of host organisms. However, the processes of bacteria flagella recognition and activation of the downstream immune system in shrimp remain unclear. To enhance comprehension of this, a ΔflhG strain was created by in-frame deletion of the flhG gene in V. alginolyticus strain HN08155. Then we utilized the transcriptome analysis to examine the different immune responses in Litopenaeus vannamei hepatopancreas after being infected with the wild type and the mutant strains. The results showed that the ΔflhG strain, unlike the wild type, lost its ability to regulate flagella numbers negatively and displayed multiple flagella. When infected with the hyperflagella-type strain, the RNA-seq revealed the upregulation of several immune-related genes in the shrimp hepatopancreas. Notably, two C-type lectins (CTLs), namely galactose-specific lectin nattectin and macrophage mannose receptor 1, and the TNF receptor-associated factor (TRAF) 6 gene were upregulated significantly. These findings suggested that C-type lectins were potentially involved in flagella recognition in shrimp and the immune system was activated through the TRAF6 pathway after flagella detection by CTLs

From optical to X-ray ghost imaging

Recent advances in ghost imaging techniques and X-ray sources such as synchrotrons and, more recently, X-ray free-electron lasers (XFEL) have made X-ray ghost imaging a growing topic of interest. One specific type of ghost imaging utilizes thermal radiation and the measurement of intensity fluctuation correlation to form a true image without the need of a lens. This technique allows for much higher resolution than traditional X-ray imaging for a mesoscopic or even a microscopic object. In addition to this benefit of not requiring a lens, a surprising experiment has shown that, when set up correctly, this type of ghost imaging can provide clear images through the measurement of intensity fluctuation correlation when traditional images through measurements of intensity are blurred due to optical turbulence and vibrations. This turbulence-free technique will help maintain the high resolution of X-ray ghost imaging. How is an image formed from fluctuations in light? And what makes it turbulence-free? Using the concept of two-photon interference, this article provides an introduction to these fundamentally interesting concepts and X-ray ghost imaging