Effect of prodigiosin on the alleviation of the intestinal inflammation of weaned rats based on 1H-NMR spectroscopy study and biochemistry indexes

Weaning results in intestinal dysfunction, mucosal atrophy, transient anorexia, and intestinal barrier defects. In this study, the effect of prodigiosin (PG) on the intestinal inflammation of weaned rats was investigated by using 1 H-NMR spectroscopy and biochemistry indexes to regulate the intestinal metabolism. After administration for 14 days, the body mass of the PG group was increased by 1.29‑ and 1.26-fold compared with those of the control and alcohol groups, respectively, using a dose of 200 μg PG·kg-1 body weight per day. PG increased organic acid content and decreased moisture, pH values, and free ammonia in feces. In addition, PG alleviated the intestinal inflammation of weaned rats. The analysis of 1 H-NMR signal peak attribution and the model validation of metabolic data of feces contents showed that PG significantly affected the metabolism of small molecular compounds in the intestinal tract of weaned rats. This study presents the promising alternative of using PG to alleviate intestinal inflammation effectively in the intestinal tract of weaned rats

Recombinant Expression of Serratia marcescens Outer Membrane Phospholipase A (A1) in Pichia pastoris and Immobilization With Graphene Oxide-Based Fe3O4 Nanoparticles for Rapeseed Oil Degumming

Enzymatic degumming is an effective approach to produce nutritional, safe, and healthy refined oil. However, the high cost and low efficiency of phospholipase limit the application of enzymatic degumming. In this study, an 879 bp outer membrane phospholipase A (A1) (OM-PLA1) gene encoding 292 amino acid residues was isolated from the genome of Serratia marcescens. The recombinant OM-PLA1 profile of appropriately 33 KDa was expressed by the engineered Pichia pastoris GS115. The OM-PLA1 activity was 21.2 U/mL with the induction of 1 mM methanol for 72 h. The expression efficiencies of OM-PLA1 were 0.29 U/mL/h and 1.06 U/mL/OD600. A complex of magnetic graphene oxide (MGO) and OM-PLA1 (MGO-OM-PLA1) was prepared by immobilizing OM-PLA1 with graphene oxide-based Fe3O4 nanoparticles by cross-linking with glutaraldehyde. The content of phosphorus decreased to 5.1 mg/kg rapeseed oil from 55.6 mg/kg rapeseed oil with 0.02% MGO-OM-PLA1 (w/w) at 50°C for 4 h. MGO-OM-PLA1 retained 51.7% of the initial activity after 13 times of continuous recycling for the enzymatic degumming of rapeseed oil. This study provided an effective approach for the enzymatic degumming of crude vegetable oil by developing a novel phospholipase and improving the degumming technology

Heterologous signal peptides-directing secretion of Streptomyces mobaraensis transglutaminase by Bacillus subtilis

Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding fragments fused to the mtg gene, were constructed in Escherichia coli and then expressed in Bacillus subtilis. Signal peptides of both WapA and AmyQ (SP wapA and SP amyQ ) were able to direct the secretion of pre-pro-MTG into the medium. A constitutive promoter (P hpaII ) was used for the expression of SP wapA -mtg, while an inducible promoter (P lac ) was used for SP amyQ -mtg. After purification from the supernatant of the culture by immobilized metal affinity chromatography and proteolysis by trypsin, 63.0 ± 0.6 mg/L mature MTG was released, demonstrated to have 29.6 ± 0.9 U/mg enzymatic activity and shown to crosslink soy protein properly. This is the first report on secretion of S. mobaraensis MTG from B. subtilis, with similar enzymatic activities and yields to that produced from Escherichia coli, but enabling a much easier purification process

The oyster genome reveals stress adaptation and complexity of shell formation

The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. Here we report the sequencing and assembly of the oyster genome using short reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response and the proteome of the shell. The oyster genome is highly polymorphic and rich in repetitive sequences, with some transposable elements still actively shaping variation. Transcriptome studies reveal an extensive set of genes responding to environmental stress. The expansion of genes coding for heat shock protein 70 and inhibitors of apoptosis is probably central to the oyster's adaptation to sessile life in the highly stressful intertidal zone. Our analyses also show that shell formation in molluscs is more complex than currently understood and involves extensive participation of cells and their exosomes. The oyster genome sequence fills a void in our understanding of the Lophotrochozoa. © 2012 Macmillan Publishers Limited. All rights reserved

Dynamic Modeling and Simulation of Quick-Setting Slurry with Spatiotemporal Rheological Properties

Abstract A major concern in underground infrastructures is how to sufficiently seal the area from water ingress. To achieve this, grout needs to be spread adequately in the surrounding fractures. Cement-based composite grouting is probably the best method for this purpose because of its lower costs and reduced environmental impacts. Chemical reaction accompanied by the flow is a prominent feature of cement-based composites. Rheological properties, especially yield stress and viscosity, are non-uniformly distributed in time and space. In this paper, the rheological properties of quick-setting slurry of cement-based composites were measured over time, and the rheology constitutive equation including time was established based on non-Newtonian fluids. Considering the rheological properties, the Lagrangian method was introduced to track their space–time distribution, and then a dynamic model of quick-setting slurry was established based on continuity equation and momentum equation. The relationship between time-varying rheological characteristics and flow field characteristics was obtained through a numerical simulation of equal pressure injection and equal flux injection. The simulation results were compared with the experimental results in the references, thereby verifying the reliability and accuracy of the model. Results show that the local erosion state and local sealing effect of grouting directly depend on the yield stress of different positions in the flow field. The yield stress of quick-setting slurry increases rapidly with time, which is more likely to affect the flow field, and the viscosity of the slurry has a small effect on the flow field

Design of a Disc-Shaped Autonomous Underwater Helicopter with Stable Fins

The autonomous underwater helicopter, shortly referred to as AUH, is a newly developed underwater platform with a unique disc shape. An autonomous underwater helicopter with a suboptimal disc shape is presented in this paper. It adopts a multirotor configuration and stable fins to overcome the shape shortcoming for motion stabilization. Its motion analysis and mathematical model have been introduced accordingly. Computational Fluid Dynamics (CFD) simulation is carried out to evaluate fins’ hydrodynamic performance. Proportional integral derivative (PID) and sliding mode fuzzy (SMF) control are adopted for controller design. Finally, the controller is applied on this AUH and extensively tested in various simulations and experiments, and the results illustrate the high stabilization and robustness of the controller and the hovering stability and manoeuvrability of AUH

Design of a Disc-Shaped Autonomous Underwater Helicopter with Stable Fins

The autonomous underwater helicopter, shortly referred to as AUH, is a newly developed underwater platform with a unique disc shape. An autonomous underwater helicopter with a suboptimal disc shape is presented in this paper. It adopts a multirotor configuration and stable fins to overcome the shape shortcoming for motion stabilization. Its motion analysis and mathematical model have been introduced accordingly. Computational Fluid Dynamics (CFD) simulation is carried out to evaluate fins’ hydrodynamic performance. Proportional integral derivative (PID) and sliding mode fuzzy (SMF) control are adopted for controller design. Finally, the controller is applied on this AUH and extensively tested in various simulations and experiments, and the results illustrate the high stabilization and robustness of the controller and the hovering stability and manoeuvrability of AUH

CRISPR-Cas9 Approach Constructed Engineered <i>Saccharomyces cerevisiae</i> with the Deletion of <i>GPD2</i>, <i>FPS1</i>, and <i>ADH2</i> to Enhance the Production of Ethanol

Bioethanol plays an important value in renewable liquid fuel. The excessive accumulation of glycerol and organic acids caused the decrease of ethanol content in the process of industrial ethanol production. In this study, the CRISPR-Cas9 approach was used to construct S. cerevisiae engineering strains by the deletion of GPD2, FPS1, and ADH2 for the improvement of ethanol production. RNA sequencing and transcriptome analysis were used to investigate the effect of gene deletion on gene expression. The results indicated that engineered S. cerevisiae SCGFA by the simultaneous deletion of GPD2, FPS1, and ADH2 produced 23.1 g/L ethanol, which increased by 0.18% in comparison with the wild-type strain with 50 g/L of glucose as substrate. SCGFA strain exhibited the ethanol conversion rate of 0.462 g per g of glucose. In addition, the contents of glycerol, lactic acid, acetic acid, and succinic acid in SCGFA decreased by 22.7, 12.7, 8.1, 19.9, and 20.7% compared with the wild-type strain, respectively. The up-regulated gene enrichment showed glycolysis, fatty acid, and carbon metabolism could affect the ethanol production of SCGFA according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Therefore, the engineering strain SCGFA had great potential in the production of bioethanol