Improving the CALA\u27s Social Media Experience: The First CALA Social Media Survey

The Chinese American Librarians Association (CALA) currently uses three social media channels, CALA Facebook, WeChat, and YouTube. In order to improve their presence and better serve the CALA members, CALA\u27s first social media survey was conducted at the end of 2016 to seek feedback from its members and friends. This poster will present the background information for the survey, its methodology, findings, recommendations and follow-up. 18 questions were compiled, added to LimeSurvey and distributed to the CALA members via its Listserv. These questions attempted to address the users\u27 social media usage, participation level, time spent on the CALA\u27s social media channels, and their perception and experience of these channels, including their understanding of the main functions of these channels, whether they felt a sense of community in these channels, and how likely they are to share information. In addition, they were also asked to provide feedback about possible future directions of the CALA’s social media in regards to the possibility of creating additional channels, the desirable content categories, and their preferred language. The responses were analyzed and illustrated using Excel. A list of recommendations were made, including experimenting with ways to get members more involved in the social networking arena by creating a private Facebook group, WeChat public account, or LinkedIn group. The ultimate goal is to encourage members to participate and engage with each other on the CALA’s social media channels. The survey was coordinated by the CALA Social Media Group and follow-up efforts will be addressed. Session photos are available at the CALA\u27s Facebook page: https://www.facebook.com/CALA-Chinese-American-Librarians-Association-281336511932864

Council on East Asian Libraries Statistics 2021-2022 for North American Institutions

See https://ceal.ku.edu/ for a full descriptionCouncil on East Asian Libraries (CEAL) 2021/2022 annual statistical data. Data collected include volumes held, volumes added gross, serials, e-journals, other library materials, electronic resources, e-books, e-journals, expenditures, fiscal support, staffing, and user services. CEAL Statistics Database is at https://ceal.ku.edu/Council on East Asian Libraries (CEAL) Statistics Committe

Loss of endothelial hypoxia inducible factor-prolyl hydroxylase 2 induces cardiac hypertrophy and fibrosis

BACKGROUND: Cardiac hypertrophy and fibrosis are common adaptive responses to injury and stress, eventually leading to heart failure. Hypoxia signaling is important to the (patho)physiological process of cardiac remodeling. However, the role of endothelial PHD2 (prolyl-4 hydroxylase 2)/hypoxia inducible factor (HIF) signaling in the pathogenesis of cardiac hypertrophy and heart failure remains elusive. METHODS AND RESULTS: Mice with Egln1Tie2Cre (Tie2-Cre-mediated deletion of Egln1 [encoding PHD2]) exhibited left ventricular hypertrophy evident by increased thickness of anterior and posterior wall and left ventricular mass, as well as cardiac fibrosis. Tamoxifen-induced endothelial Egln1 deletion in adult mice also induced left ventricular hypertrophy and fibrosis. Additionally, we observed a marked decrease of PHD2 expression in heart tissues and cardiovascular endothelial cells from patients with cardiomyopathy. Moreover, genetic ablation of Hif2a but not Hif1a in Egln1Tie2Cre mice normalized cardiac size and function. RNA sequencing analysis also demonstrated HIF-2α as a critical mediator of signaling related to cardiac hypertrophy and fibrosis. Pharmacological inhibition of HIF-2α attenuated cardiac hypertrophy and fibrosis in Egln1Tie2Cre mice. CONCLUSIONS: The present study defines for the first time an unexpected role of endothelial PHD2 deficiency in inducing cardiac hypertrophy and fibrosis in an HIF-2α– dependent manner. PHD2 was markedly decreased in cardiovascular endothelial cells in patients with cardiomyopathy. Thus, targeting PHD2/HIF-2α signaling may represent a novel therapeutic approach for the treatment of pathological cardiac hypertrophy and failure

Petrogenesis of the Eocene Highly Fractionated Granite Porphyry with REE Tetrad Effect: An Example from Western Yunnan, Southeastern Tibetan Plateau

Highly fractionated granites are widely distributed in the crust and provide unique windows into magmatic evolution. This study reports petrography, zircon U–Pb ages, trace elemental, and Hf isotopic, as well as whole-rock elemental and Nd isotopic data of highly fractionated granite porphyries from the Shiguanshan area in western Yunnan, southeastern Tibet. The granite porphyries were formed at 34.0 ± 0.3 Ma in a post-collisional setting. They are strongly peraluminous (A/CNK = 1.95–2.80), have high SiO2 content (SiO2 = 78.16–79.13 wt.%) and zircon saturation temperatures (803–829 °C, average 819 °C), and low MgO, with pronounced enrichment in Pb, U, Th, and Rb, and depletion in Ti, Eu, P, Sr, and Ba, and belong to highly fractionated A-type granites. These rocks define linear trends on Harker diagrams and display similar enriched whole-rock Nd isotopic (εNd(t) = −12.8 to −12.3) and zircon Hf isotopic (εHf(t) = −10.4 to −8.8) compositions compared to the published data of coeval mantle-derived syenite porphyries, which can be attributed to fractional crystallization processes. A quantitative model suggests that the Shiguanshan granite porphyries likely formed through the fractionation process of a mineral assemblage consisting of plagioclase, K-feldspar, biotite, and amphibole (in a ratio of 40:30:25:5), with fractionation degrees of 50%–55%. The magmatic textures and zircons, decoupling between the REE tetrad effect and fractionation of twin-elements, along with the modeling result of Rayleigh fractionation, suggest that the REE tetrad effect in the Shiguanshan granite porphyries may be caused by fractionation of accessory minerals. Our data, along with regional observations, propose that the generation of these granite porphyries is possibly related to lithospheric removal following the Indo–Asia collision

RNA-seq analysis of chlorogenic acid intervention in duck embryo fibroblasts infected with duck plague virus

Abstract Introduction Chlorogenic acid, the primary active component in Chinese medicines like honeysuckle, exhibits anti-inflammatory and antiviral effects. It has been demonstrated that chlorogenic acid effectively prevents and treats Duck enteritis virus (DEV) infection. This study aims to further elucidate the mechanism by which chlorogenic acid prevents DEV infection. Methods Duck embryo fibroblast (DEF) cells were pre-treated with chlorogenic acid before being infected with DEV. Cell samples were collected at different time points for transcriptomic sequencing, while qPCR was used to detect the proliferation of DEV. Additionally, 30-day-old ducks were treated with chlorogenic acid, and their lymphoid organs were harvested for histopathological sections to observe pathological damage. The proliferation of DEV in the lymphoid organs was also detected using qPCR Based on the transcriptomic sequencing results, NF-κB1 gene was silenced by RNAi technology to analyze the effect of NF-κB1 gene on DEV proliferation. Results Compared to the viral infection group, DEF cells in the chlorogenic acid intervention group exhibited significantly reduced DEV load (P < 0.05). Transcriptomic sequencing results suggested that chlorogenic acid inhibited DEV proliferation in DEF cells by regulating NF-κB signaling pathway. The results of RNAi silencing suggested that in the three treatment groups, compared with the DEV experimental group, there was no significant difference in the effect of pre-transfection after transfection on DEV proliferation, while both the pre-transfection after transfection and the simultaneous transfection group showed significant inhibition on DEV proliferation Furthermore, compared to the virus infection group, ducks in the chlorogenic acid intervention group showed significantly decreased DEV load in their lymphoid organs (P < 0.05), along with alleviated pathological damage such as nuclear pyretosis and nuclear fragmentation. Conclusions Chlorogenic acid effectively inhibits DEV proliferation in DEF and duck lymphatic organs, mitigates viral-induced pathological damage, and provides a theoretical basis for screening targeted drugs against DEV

Untargeted metabolomics of the intestinal tract of DEV-infected ducks

Abstract Introduction Duck enteritis virus (DEV) mainly causes infectious diseases characterized by intestinal haemorrhage, inflammation and parenchymal organ degeneration in ducks and other poultry. However, the mechanism by which it causes intestinal damage in ducks is not well understood. Metabolomics can provide an in-depth understanding of the full complexity of the disease. Methods In this study, 24 clinically healthy green-shell ducks (weight 1.5 kg ± 20 g) were randomly divided into 2 groups (experimental group, 18; control group, 6). The experimental group was intramuscularly injected with 0.2 mL of DEV virus in solution (TCID50 3.16 × 108 PFU/mL), and the control group was injected with 0.2 mL of sterile normal saline. Duck duodenum and ileum tissue samples were collected at 66 h, 90 h and 114 h post-injection (12 h of fasting before killing), and metabolomics analysis of duck duodenum and ileum tissues at the three time points (66, 90, 114 h) was performed by liquid chromatography–mass spectrometry (LC–MS) to screen for and analyse the potential differentiated metabolites and related signalling pathways. Results Screening was performed in the positive/negative mode (Pos: Positive ion mode; the ionization of substances at the ion source with positive ions such as H+, NH4 +, Na+ and K+; Neg: Negative ion mode; the ionization of substances at the ion source with negative ions such as Cl−, OAc−), and compound abundance was compared to that in the control group. The total number of differentially abundant compounds in the duodenum at 66 h, 90 h and 114 h of DEV infection gradually increased, and metabolites such as cytidine, 2′-deoxyriboside and 4-guanidinobutyric acid were differentially abundant metabolites common to all three time periods. The metabolic pathways related to inflammatory response and immune response were tryptophan acid metabolism, cysteine-methionine metabolism, histidine metabolism and other amino acid metabolism and fat metabolism. Among them, the metabolic pathways with more differentially abundant metabolites were amino acid biosynthesis, cysteine and methionine metabolism, tryptophan metabolism, unsaturated fatty acid biosynthesis and purine metabolism, and the metabolic pathways with more enrichment factors were the IgA-related intestinal immune network pathway and lysosome pathway. Compared with the control group, there were 16 differentially abundant metabolites in the ileum tissue of DEV-infected ducks at 66 h of infection, 52 at 90 h of infection, and 40 at 14 h of infection with TD114. The metabolic pathways with more enriched differentially abundant metabolites were pyrimidine metabolism, tyrosine metabolism, phenylalanine metabolism and tryptophan biosynthesis. The metabolic pathways with the most enrichment factors were the mTOR signalling pathway, ferroptosis pathway, tryptophan metabolism pathway and caffeine metabolism pathway. Conclusion Comparative analysis showed that the number of differentially abundant metabolites in the duodenum and ileum differed to some extent after DEV infection, with significantly more differentially abundant metabolites in duodenal tissues and fewer in ileal tissues; after DEV infection, the highest number of differentially abundant metabolites was obtained at 114 h of DEV infection, followed by the second highest at 90 h of infection and the lowest at 66 h of infection. The common differentially abundant metabolites in duodenal and ileal tissues were prostaglandins, arachidonic acid, and arachidonic ethanolamine. The main metabolic pathways in the duodenum were the IgA-associated intestinal immune network pathway and the lysosomal pathway, and the metabolic pathways with more enriched factors in the ileum were the mTOR signalling pathway, the ferroptosis pathway, and the tryptophan metabolism pathway

A Carbon 21 Steroidal Glycoside with Pregnane Skeleton from <em>Cynanchum atratum</em> Bunge Promotes Megakaryocytic and Erythroid Differentiation in Erythroleukemia HEL Cells through Regulating Platelet-Derived Growth Factor Receptor Beta and JAK2/STAT3 Pathway

Erythroleukemia is a rare form of acute myeloid leukemia (AML). Its molecular pathogenesis remains vague, and this disease has no specific therapeutic treatments. Previously, our group isolated a series of Carbon 21 (C-21) steroidal glycosides with pregnane skeleton from the root of Cynanchum atratum Bunge. Among them, we found that a compound, named BW18, can induce S-phase cell cycle arrest and apoptosis via the mitogen-activated protein kinase (MAPK) pathway in human chronic myeloid leukemia K562 cells. However, its anti-tumor activity against erythroleukemia remains largely unknown. In this study, we aimed to investigate the anti-erythroleukemia activity of BW18 and the underlying molecular mechanisms. Our results demonstrated that BW18 exhibited a good anti-erythroleukemia activity in the human erythroleukemia cell line HEL and an in vivo xenograft mouse model. In addition, BW18 induced cell cycle arrest at the G2/M phase and promoted megakaryocytic and erythroid differentiation in HEL cells. Furthermore, RNA sequencing (RNA-seq) and rescue assay demonstrated that overexpression of platelet-derived growth factor receptor beta (PDGFRB) reversed BW18-induced megakaryocytic differentiation in HEL cells, but not erythroid differentiation. In addition, the network pharmacology analysis, the molecular docking and cellular thermal shift assay (CETSA) revealed that BW18 could inactivate Janus tyrosine kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway, which might mediate BW18-induced erythroid differentiation. Taken together, our findings elucidated a novel role of PDGFRB in regulating erythroleukemia differentiation and highlighted BW18 as an attractive lead compound for erythroleukemia treatment

Table_1_Discriminating the non-coordination between ecosystem service value and economic development and its spatial characteristics in central Yunnan urban agglomeration in the recent 30 years.docx

Environmental issues have become one of the important topics affecting China and the world. As the world’s most populous country and the second-largest economy, China’s environmental conditions have significant impacts on global ecosystems and sustainable development. To accurately determine the relationship between economic development and the environment, explore the non-coordination and spatial characteristics between the two, and promote regional sustainable development. This study is based on land-use data from four periods: 1990, 2000, 2010, and 2020. It uses the dynamic measurement and value equivalent method to explore the characteristics of land-use changes and measure the value of ecosystem services in the Central Yunnan Urban Agglomeration. At the same time, the study uses the non-coordination degree model and hotspot analysis tools to identify the non-coordination between Ecosystem Service Value and economic development (Gross Domestic Product), and explore the spatial characteristics between them. The research results show that: (1) The main land-use types include cultivated land, forest land, and grassland, which account for more than 90% of the total proportion. The land types with a small proportion in the region are water areas, construction land, and unused land. (2) The development level of ecosystem service value presents four types of changes: fluctuating increase, fluctuating decrease, continuous increase, and continuous decrease. The regional spatial distribution shows obvious regional heterogeneity, overall presenting a spatial distribution pattern of “low in the middle, high around.” (3) The average index of non-coordination degree from 1990 to 2020 is 0.591, 0.635, 0.584, and 0.565, showing a coordinated non-coordination coupling type. The regional space presents a spatial pattern of “high in the central and western regions, low in the northeastern and southern regions.” (4) The hotspots of non-coordination are mainly concentrated in the central and northwest regions of the Central Yunnan Urban Agglomeration, while the coldspots of non-coordination are mainly distributed in the northeastern, western, and southern regions of the Central Yunnan Urban Agglomeration, showing a regular agglomeration distribution. This study suggests that it is necessary to balance and coordinate the relationship between regional economic development and the environment.</p

Waste tire upcycling for the efficient electrogeneration of H₂O₂ in advanced degradation of the antibiotic tinidazole by electro-Fenton process

The electrochemical production of H2O2 using small- and medium-size modular devices has emerged as aninteresting approach for water treatment. In this work, waste tire-derived carbon (WTC) has been innovativelyemployed as a cheap and recycled electroactive material to manufacture a gas-diffusion cathode. It was foundthat acidification with HNO3 significantly enhanced the H2O2 production, attaining a concentration of up to1161 mg/L with a current efficiency around 40 %–60 %. These results were superior to those obtained bytreating the WTC with H2SO4 or KOH, being also better than the values reached with reported carbons. Liner andcyclic voltammetries informed about the oxygen reduction reaction profiles; transmission electron microscopyrevealed the morphological features of the synthesized carbons, whereas other properties were obtained byBrunauer-Emmett-Teller analysis, Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy.The cathode was further employed to degrade tinidazole (TNZ), a typical nitroimidazole antibiotic, inwater by electro-Fenton at pH 3.0, producing more than 10 mM H2O2. The effect of solution pH, Fe2+ dosage andinitial TNZ concentration was investigated. Under optimal conditions (0.5 mM Fe2+ and 20 mA/cm2), thetreatment of solutions containing 20 mg/L TNZ at pH 3.0 yielded a pseudo-first-order constant (k1) of 0.449min-1. Besides, the degradation pathways were proposed according to the 7 identified transformation products.This study demonstrates that waste tires can become a source of inexpensive carbon able to catalyze the in-situH2O2 synthesis for water decontamination, in excellent agreement with new policies on circular economy