Dichloridobis[2-(2-fur­yl)-1-(2-furylmeth­yl)-1H-benzimidazole-κN 3]cadmium(II)

In the title complex, [CdCl2(C16H12N2O2)2], the CdII ion exhibits site symmetry 2. It shows a distorted tetra­hedral coordination defined by two N atoms from symmetry-related 2-(2-fur­yl)-1-(2-furylmeth­yl)-1H-benzimidazole ligands and by two symmetry-related Cl atoms. Intra­molecular C—H⋯O hydrogen bonds stabilize the mol­ecular configuration. Adjacent mol­ecules are linked through C—H⋯Cl hydrogen bonds into a network structure

Multigene Editing Via CRISPR/Cas9 Guided By a Single-sgRNA Seed in Arabidopsis

We report that a single-sgRNA seed is capable of guiding CRISPR/Cas9 to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a single sgRNA seed-induced gene editing system might be also applicable to modify other homologous genes or even less-homologous sequences for multiple gene-editing in plants and other organisms. [Abstract copyright: This article is protected by copyright. All rights reserved.

Magnetic hysteresis up to 80 kelvin in a dysprosium metallocene single-molecule magnet

Single-molecule magnets (SMMs) containing only one metal center may represent the lower size limit for molecule-based magnetic information storage materials. Their current drawback is that all SMMs require liquid-helium cooling to show magnetic memory effects. We now report a chemical strategy to access the dysprosium metallocene cation [(CpiPr5)Dy(Cp*)]+ (CpiPr5 = penta-iso-propylcyclopentadienyl, Cp* = pentamethylcyclopentadienyl), which displays magnetic hysteresis above liquid-nitrogen temperatures. An effective energy barrier to reversal of the magnetization of Ueff = 1,541 cm–1 is also measured. The magnetic blocking temperature of TB = 80 K for this cation overcomes an essential barrier towards the development of nanomagnet devices that function at practical temperatures

The effects of different extraction methods on the physicochemical property and antioxidant activity of <i>Amygdalus pedunculatus</i> seed oil

The oil extracted from Amygdalus pedunculatus (A. pedunculatus) seeds is rich in nutrients. The method of oil extraction is very crucial for preserving its nutrients. The objective of the present study was to compare A. pedunculatus seed oil (APO) samples extracted by different techniques including aqueous enzymatic extraction (AEE), cold-press (CP), supercritical fluid extraction (SFE), and Soxhlet extraction (SE). Physicochemical properties and nutrients (fatty acids, triacylglycerol, polyphenol, tocopherol and phytosterol) of the oils were analyzed. Antioxidant activity was measured by DPPH, ABTS·+ radical scavenging capacity and reducing power assays. The results indicated that SFE was found to be the optimum method for APO extraction with higher nutrient contents as well as better DPPH, ABTS scavenging capacities and reducing power. APO is beneficial to human health, and it has potential to be used in nutraceutical industries

The recent progress of peptide regulators for the Wnt/β-catenin signaling pathway

Wnt signaling plays an important role in many biological processes such as stem cell self-renewal, cell proliferation, migration, and differentiation. The β-catenin-dependent signaling pathway mainly regulates cell proliferation, differentiation, and migration. In the Wnt/β-catenin signaling pathway, the Wnt family ligands transduce signals through LRP5/6 and Frizzled receptors to the Wnt/β-catenin signaling cascades. Wnt-targeted therapy has garnered extensive attention. The most commonly used approach in targeted therapy is small-molecule regulators. However, it is difficult for small-molecule regulators to make great progress due to their inherent defects. Therapeutic peptide regulators targeting the Wnt signaling pathway have become an alternative therapy, promising to fill the gaps in the clinical application of small-molecule regulators. In this review, we describe recent advances in peptide regulators for Wnt/β-catenin signaling

A 95 GHz Class I Methanol Maser Survey Toward A Sample of GLIMPSE Point Sources Associated with BGPS Clumps

We report a survey with the Purple Mountain Observatory (PMO) 13.7-m radio telescope for class I methanol masers from the 95 GHz (8_0 - 7_1 A^+) transition. The 214 target sources were selected by combining information from both the Spitzer GLIMPSE and 1.1 mm BGPS survey catalogs. The observed sources satisfy both the GLIMPSE mid-IR criteria of [3.6]-[4.5]>1.3, [3.6]-[5.8]>2.5, [3.6]-[8.0]>2.5 and 8.0 um magnitude less than 10, and also have an associated 1.1 mm BGPS source. Class I methanol maser emission was detected in 63 sources, corresponding to a detection rate of 29% for this survey. For the majority of detections (43), this is the first identification of a class I methanol maser associated with these sources. We show that the intensity of the class I methanol maser emission is not closely related to mid-IR intensity or the colors of the GLIMPSE point sources, however, it is closely correlated with properties (mass and beam-averaged column density) of the BGPS sources. Comparison of measures of star formation activity for the BGPS sources with and without class I methanol masers indicate that the sources with class I methanol masers usually have higher column density and larger flux density than those without them. Our results predict that the criteria log(S_{int})22.1, which utilizes both the integrated flux density (S_{int}) and beam-averaged column density (N_{H_{2}}^{beam}) of the BGPS sources, are very efficient for selecting sources likely to have an associated class I methanol maser. Our expectation is that searches using these criteria will detect 90% of the predicted number of class I methanol masers from the full BGPS catalog (~ 1000), and do so with a high detection efficiency (~75%).Comment: Accepted for publication in ApJ Supplement. 58 pages, 12 figures, 7 table