The oil extracted from Amygdalus pedunculatus (A. pedunculatus) seeds is rich in nutrients. The method of oil extraction is very crucial for preserving its nutrients. The objective of the present study was to compare A. pedunculatus seed oil (APO) samples extracted by different techniques including aqueous enzymatic extraction (AEE), cold-press (CP), supercritical fluid extraction (SFE), and Soxhlet extraction (SE). Physicochemical properties and nutrients (fatty acids, triacylglycerol, polyphenol, tocopherol and phytosterol) of the oils were analyzed. Antioxidant activity was measured by DPPH, ABTS·+ radical scavenging capacity and reducing power assays. The results indicated that SFE was found to be the optimum method for APO extraction with higher nutrient contents as well as better DPPH, ABTS scavenging capacities and reducing power. APO is beneficial to human health, and it has potential to be used in nutraceutical industries
