Nudel functions in membrane traffic mainly through association with Lis1 and cytoplasmic dynein

Nudel and Lis1 appear to regulate cytoplasmic dynein in neuronal migration and mitosis through direct interactions. However, whether or not they regulate other functions of dynein remains elusive. Herein, overexpression of a Nudel mutant defective in association with either Lis1 or dynein heavy chain is shown to cause dispersions of membranous organelles whose trafficking depends on dynein. In contrast, the wild-type Nudel and the double mutant that binds to neither protein are much less effective. Time-lapse microscopy for lysosomes reveals significant reduction in both frequencies and velocities of their minus end–directed motions in cells expressing the dynein-binding defective mutant, whereas neither the durations of movement nor the plus end–directed motility is considerably altered. Moreover, silencing Nudel expression by RNA interference results in Golgi apparatus fragmentation and cell death. Together, it is concluded that Nudel is critical for dynein motor activity in membrane transport and possibly other cellular activities through interactions with both Lis1 and dynein heavy chain

Research on Acquisition and Communication Technology of Marine Equipment Fault Diagnosis Information for Vessels in Inland Rivers

Abstract: This study describes a system for the analysis of the fault diagnosis of marine equipments in inland rivers. Since the normal operation of the marine equipments significantly influences the ship safety, this study develops an efficient fault diagnosis information system for the condition monitoring and fault diagnosis of marine equipments. In this new system, field bus and ship-to-shore communication technologies have been integrated for the fault diagnosis information acquisition. Then the application of network bus, including CAN and RS485, has been employed to connect the fault diagnosis information with Ethernet in the ship. Lastly, for the real time and wireless transmission of the fault information, the Automatic Identification System (AIS) technology has been adopted to provide accurate and reliable fault diagnosis information transmission from ships to onshore diagnosis center. A comprehensive study of the application of proposed fault diagnosis information system has been implemented for remote diagnosis of marine equipments. The analysis results demonstrate that the newly developed fault diagnosis information system can enhance the fault diagnosis precision and hence is competent for the condition monitoring and fault diagnosis of marine equipments in inland rivers

A Complex of Two Centrosomal Proteins, CAP350 and FOP, Cooperates with EB1 in Microtubule Anchoring

The anchoring of microtubules (MTs) to subcellular structures is critical for cell shape, polarity, and motility. In mammalian cells, the centrosome is a prominent MT anchoring structure. A number of proteins, including ninein, p150(Glued), and EB1, have been implicated in centrosomal MT anchoring, but the process is far from understood. Here we show that CAP350 and FOP (FGFR1 oncogene partner) form a centrosomal complex required for MT anchoring. We show that the C-terminal domain of CAP350 interacts directly with FOP and that both proteins localize to the centrosome throughout the cell cycle. FOP also binds to EB1 and is required for localizing EB1 to the centrosome. Depletion of either CAP350, FOP, or EB1 by siRNA causes loss of MT anchoring and profound disorganization of the MT network. These results have implications for the mechanisms underlying MT anchoring at the centrosome and they attribute a key MT anchoring function to two novel centrosomal proteins, CAP350 and FOP

SHP-2 restricts apoptosis induced by chemotherapeutic agents via Parkin-dependent autophagy in cervical cancer

Abstract Background Autophagy is a cell degradation pathway that eliminates damaged or unwanted proteins and organelles. Autophagy protects cells from chemotherapeutic agents by scavenging damaged mitochondria. Methods Plasmid transfection and shRNA were used to regulate SHP-2 expression. Annexin V/PI staining were employed to analysis apoptosis. Flow cytometry was used to analyse intracellular calcium level and ROS. Immunofluorescence was used to detect mitochondria membrane potential, autophagy and Parkin translocation. Results In cervical cancer, we found that SHP-2 suppressed apoptosis induced by Oxaliplatin and 5-FU. Further studies have found that SHP-2 protects against mitochondrial damage. This role of SHP-2 is associated with the activation of autophagy. In addition, SHP-2 degraded impaired mitochondria dependent on the ubiquitin ligase function of Parkin. Conclusions These results suggest that SHP-2 inhibits the apoptosis induced by chemotherapeutic drugs through activating autophagy to degrade damaged mitochondria and ubiquitin ligase Parkin involved in SHP-2 induced autophagy

Hazard Assessment for Biomass Gasification Station Using General Set Pair Analysis

Hazard assessment is critical for a biomass gasification station because it includes various hazardous factors. Set pair analysis (SPA) is a convenient and effective method for hazard assessment, but it has limitations, including (1) the inability to reflect the difference when the data belong to the same hazard grade and (2) the assessment results lack precision and accuracy. This study proposes an improved method designated as general set pair analysis (GSPA). Connection measure degree (CMD), which is based on the cosine function, as well as weighting deviation degree (WDD), relative membership degree (RMD), and comprehensive index (CI) were proposed in GSPA, and the algorithms were generated. The calculated results of these different methods can be utilized to overcome the shortcomings of SPA during hazard assessment. A case study of two biomass gasification stations in Shenyang City, Liaoning Province, Northeast China, was performed. The hazard assessment results of the GSPA were compared with those of SPA. The results showed that GSPA is a more effective, precise, and accurate method for hazard assessment of a biomass gasification station

Education and Instruction for Circular Economy: A Review on Drivers and Barriers in Circular Economy Implementation in China

As a new sustainable alternative to linear economics, the circular economy (CE) is bound to be accompanied by the requirements of the existing production model, science and technology, as well as the public perception of CE. To expose the potential impact of higher education on CE transition, this study systematically reviewed the relevant literature on the drivers and barriers to CE with a mixed analytical method. CiteSpace was used to perform bibliometric analysis and visual presentation of 367 literature accurately retrieved by search formula from two international influential databases, Web of Science and Scopus, and two significant Chinese databases, CNKI and CQVIP. In the results, we identified the number of publications and citations, the annual distribution of publications, annual citation trends, county distribution of publication contribution, the most-cited articles, and the co-citation networks. Moreover, we clarified the main drivers and barriers to CE in China by conducting qualitative content of the keywords related to driver and barrier factors in the literature. The results suggest that higher education has a positive influence on strengthening the main drivers and eliminating the main barriers, which implies that higher education has bright prospects in speeding up the CE transition. To maximize the effect of higher education for CE, we suggested university leaders and lecturers should adopt effective education and instruction measures in improving the performance of education activities for CE, so that the future leaders of various industries in universities could construct CE values and behavior patterns to achieve the replacement of traditional linear economy

Prenatal Diagnosis: The Main Advances in the Application of Identification of Biomarkers Based on Multi-Omics

Prenatal diagnosis is to make the diagnosis of fetal structural abnormalities, genetic diseases, and pregnancy-related diseases before birth thus could offer evidence for intrauterine treatment or selectively termination of pregnancy. Up to now, researchers have applied multi-omics, including genomics, transcriptomics, and proteomics, in the discovery of prenatal diagnostic biomarkers. They have found some candidate biomarkers for aneuploids, preeclampsia, intrauterine growth retardation, and congenital structural abnormalities. With the momentous progress of biomarkers’ identification based on multi-omics for prenatal diagnosis, noninvasive prenatal testing (NIPT) has experienced tremendous progress and is revolutionizing prenatal screening and diagnosis over the past few decades. Extensive studies have also demonstrated the value of biomarkers. In particular, cell-free DNA (cfDNA), allows for a definitive diagnosis in early pregnancy for fetal diseases, including Down syndrome and other common aneuploidies. The cfDNA can be extracted from maternal plasma, posing no risk of miscarriage compared to the traditional invasive diagnosis directly analyzing fetal cells from amniocentesis or chorionic villus sampling. In this review, we would discuss the main advances, strengths, and limitations in the application of biomarkers for prenatal diagnosis along with the analysis of several representative fetal diseases