Flavonoides presentes nos extratos da campomanesia xanthocarpa Berg / Flavonoids present in extracts of campomanesia xanthocarpa Berg

O aumento do interesse por compostos bioativos de origem natural com alta capacidade antioxidante aumentou consideravelmente nas duas últimas décadas, principalmente devido ao seu potencial na prevenção e tratamento auxiliar de doenças inflamatórias crônicas. Em vista deste fato, o presente estudo buscou quantificar os flavonoides presentes na planta guabirobeira. Os extratos foram elaborados com as folhas e polpa da C. xanthocarpa, através do método extrativo sob refluxo em aparelho Soxhlet a 78,4 oC utilizando como solvente o etanol 70%. O extrato SF-et apresentou maior quantidade de flavonoides. O composto bioativo observado no presente estudo apresentou maior concentração no extrato da folha da C. xanthocarpa extraída pelo solvente etanol 70%. 

Lymphocytes transfer [(14)C]-labeled fatty acids to skeletal muscle in culture; modulation by exercise

Previous studies have shown that lipids are transferred from lymphocytes (Ly) to different cell types including macrophages. enterocytes, and pancreatic beta cells in co-culture This study investigated whether [(14)C]-labeled fatty acids (FA) can be transferred from Ly to skeletal muscle (SM), and the effects of exercise on such phenomenon Ly obtained from exercised (EX) and control (C) male Wistar rats were preloaded with the [(14)C]-labeled free FA palmitic (PA), oleic (OA), linoleic (LA), or arachidonic (AA) Radioactively loaded Ly were then co-cultured with SM from the same Ly donor animals Substantial amounts of FA were transferred to SM being the profile PA = OA > AA > LA to the C group. and PA > OA > LA > AA to the EX group These FA were incorporated predominantly as phospholipids (PA = 66 75%: OA = 63 09%, LA = 43 86%, AA - 47 40%) in the C group and (PA = 63 99% OA = 52 72%, LA = 55 99%, AA = 63 40%) in the EX group Also in this group, the remaining radioactivity from AA, LA, and OA acids was mainly incorpoiated in structural and energetic lipids These results support the hypothesis that Ly are able to export lipids to SM in co-culture Furthermore. exercise modulates the lipid transference profile, and its incorporation on SM The overall significance of this phenomenon in vivo remains to be elucidated. Copyright (C) 2010 John Wiley & Sons, LtdCAPESCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Counsel of Technological and Scientific Development (CNPq