404 research outputs found
Glycosylation pattern of brush border-associated glycoproteins in enterocyte-like cells: involvement of complex-type N-glycans in apical trafficking
We have previously reported that galectin-4, a tandem repeat-type galectin, regulates the raft-dependent delivery of glycoproteins to the apical brush border membrane of enterocyte-like HT-29 cells. N-Acetyllactosamine-containing glycans, known as galectin ligands, were found enriched in detergent-resistant membranes. Here, we analyzed the potential contribution of N-and/ or O-glycans in this mechanism. Structural studies were carried out on the brush border membrane-enriched fraction using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and nano-ESI-QTOF-MS/MS. The pattern of N-glycans was very heterogeneous, with the presence of high mannose- and hybrid-type glycans as well as a multitude of complex-type glycans. In contrast, the pattern of O-glycans was very simple with the presence of two major core type 1 O-glycans, sialylated and bisialylated T-antigen structures {[}Neu5Ac alpha 2-3Gal beta 1-3GalNAc-ol and Neu5Ac alpha 2-3Gal beta 1 -3(Neu5Ac alpha 2-6)GalNAc-ol]. Thus, N-glycans rather than O-glycans contain the N-acetyllactosamine recognition signals for the lipid raft-based galectin-4-dependent apical delivery. In the presence of 1-deoxymannojirimycin, a drug which inhibits the generation of hybrid-type or complex type N-glycans, the extensively O-glycosylated mucin-like MUC1 glycoprotein was not delivered to the apical brush border but accumulated inside the cells. Altogether, our data demonstrate the crucial role of complex N-glycans in the galectin-4-dependent delivery of glycoproteins to the apical brush border membrane of enterocytic HT-29 cells
Neuromuscular synaptic function in mice lacking major subsets of gangliosides
Gangliosides are a family of sialylated glycosphingolipids enriched in the outer leaflet of neuronal membranes, in particular at synapses. Therefore, they have been hypothesized to play a functional role in synaptic transmission. We have measured in detail the electrophysiological parameters of synaptic transmission at the neuromuscular junction (NMJ) ex vivo of a GD3-synthase knockout mouse, expressing only the O- and a-series gangliosides, as well as of a GM2/GD2-synthase*GD3-synthase double-knockout (dKO) mouse, lacking all gangliosides except GM3. No major synaptic deficits were found in either null-mutant. However, some extra degree of rundown of acetylcholine release at high intensity use was present at the dKO NMJ and a temperature-specific increase in acetylcholine release at 35 °C was observed in GD3-synthase knockout NMJs, compared with wild-type. These results indicate that synaptic transmission at the NMJ is not crucially dependent on the particular presence of most ganglioside family members and remains largely intact in the sole presence of GM3 ganglioside. Rather, presynaptic gangliosides appear to play a modulating role in temperature- and use-dependent fine-tuning of transmitter output
Characterization of halogen-bridged binuclear metal complexes as hybridized two-band materials
We study the electronic structure of halogen-bridged binuclear metal (MMX)
complexes with a two-band Peierls-Hubbard model. Based on a symmetry argument,
various density-wave states are derived and characterized. The ground-state
phase diagram is drawn within the Hartree-Fock approximation, while the thermal
behavior is investigated using a quantum Monte Carlo method. All the
calculations conclude that a typical MMX compound Pt_2(CH_3CS_2)_4I should
indeed be regarded as a d-p-hybridized two-band material, where the oxidation
of the halogen ions must be observed even in the ground state, whereas another
MMX family (NH_4)_4[Pt_2(P_2O_5H_2)_4X] may be treated as single-band
materials.Comment: 16 pages, 11 figures embedded, to be published in Phys. Rev.
Search for B+ -> D*+ pi0 decay
We report on a search for the doubly Cabibbo suppressed decay B+ -> D*+ pi0,
based on a data sample of 657 million BBbar pairs collected at the Upsilon(4S)
resonance with the Belle detector at the KEKB asymmetric energy e+ e- collider.
We find no significant signal and set an upper limit of Br(B+ -> D*+ pi0) < 3.6
x 10^-6 at the 90% confidence level. This limit can be used to constrain the
ratio between suppressed and favored B -> D* pi decay amplitudes, r < 0.051, at
the 90% confidence level.Comment: 5pages, 2figures, submitted to PRL (v1); PRL published version (v2:
minor corrections in the text
Search for Resonant Decays at Belle
We report measurements and searches for resonant decays where is a
meson or
the X(3872) particle.Comment: accepted by Physics Letters
Observation of Ds1(2536)+ -> D+pi-K+ and angular decomposition of Ds1(2536)+ -> D*+K0S
Using 462/fb of e+e- annihilation data recorded by the Belle detector, we
report the first observation of the decay Ds1(2536)+ -> D+pi-K+. The ratio of
branching fractions B(Ds1+ -> D+pi-K+)/B(Ds1+ -> D*+K0) is measured to be
(3.27+-0.18+-0.37)%. We also study the angular distributions in the Ds1(2536)+
-> D*+K0S decay and measure the ratio of D- and S-wave amplitudes. The S-wave
dominates, with a partial width of Gamma_S/Gamma_total=0.72+-0.05+-0.01.Comment: Submitted to Phys.Rev.D 16 pages, 6 figures, 3 table
Measurement of CP asymmetry in Cabibbo suppressed D0 decays
We measure the CP-violating asymmetries in decays to the D0 -> K+K- and D0 ->
pi+pi- CP eigenstates using 540 fb^{-1} of data collected with the Belle
detector at or near the Upsilon(4S) resonance. Cabibbo-favored D0 -> K-pi+
decays are used to correct for systematic detector effects. The results,
A_{CP}^{KK} = (-0.43 +- 0.30 +- 0.11)% and A_{CP}^{pipi} = (+0.43 +- 0.52 +-
0.12)%, are consistent with no CP violation.Comment: Submitted to Phys. Lett.
Precise measurement of hadronic tau-decays with an eta meson
We have studied hadronic tau decay modes involving an eta meson using 490
fb^{-1} of data collected with the Belle detector at the KEKB asymmetric-energy
e+e- collider. The following branching fractions have been measured: B(tau- ->
K- eta nu)=(1.58 +- 0.05 +- 0.09)x 10^{-4}, B(tau- -> K- pi0 eta nu)=(4.6 +-
1.1 +- 0.4)x 10^{-5}, B(tau- -> pi- pi0 eta nu)=(1.35 +- 0.03 +- 0.07)x
10^{-3}, B(tau- -> pi- KS eta nu)=(4.4 +- 0.7 +- 0.2)x 10^{-5}, and B(tau- ->
K^{*-} eta nu)=(1.34 +- 0.12 +- 0.09)x 10^{-4}. These results are substantially
more precise than previous measurements. The new measurements are compared with
theoretical calculations based on the CVC hypothesis or the chiral perturbation
theory. We also set upper limits on branching fractions for tau decays into K-
KS eta nu, pi- KS pi0 eta nu, K- eta eta nu, pi- eta eta nu and non-resonant K-
pi^0 eta nu final states.Comment: 24 pages, 7 figure
Measurements of exclusive B_s^0 decays at the Y(5S) resonance
Several exclusive decays are studied using a 1.86 fb-1 data sample
collected at the Y(5S) resonance with the Belle detector at the KEKB asymmetric
energy e^+ e^- collider. In the decay mode we find 10
candidates and measure the corresponding branching fraction. Combining
the B_s^0 -> D_s^{(*)-} \pi^+, B_s^0 -> D_s^{(*)-} \rho^+, B_s^0 -> J/\psi \phi
and B_s^0 -> J/\psi \eta decay modes, a significant signal is observed.
The ratio \sigma (e^+ e^- -> B_s^* \bar{B}_s^*) / \sigma (e^+ e^- -> B_s^{(*)}
\bar{B}_s^{(*)}) = (93^{+7}_{-9} \pm 1)% is obtained at the Y(5S) energy,
indicating that meson production proceeds predominantly through the
creation of pairs. The and meson masses are
measured to be M(B_s^0)=(5370 \pm 1 \pm 3)MeV/c^2 and M(B_s^*)=(5418 \pm 1 \pm
3)MeV/c^2. Upper limits on the B_s^0 -> \gamma \gamma, B_s^0 -> \phi \gamma,
B_s^0 -> K^+ K^- and B_s^0 -> D_s^{(*)+} D_s^{(*)-} branching fractions are
also reported.Comment: 9 pages, 5 figures, published in Phys. Rev. D76, 012002 (2007
Measurement of K^+K^- production in two-photon collisions in the resonant-mass region
K^+K^- production in two-photon collisions has been studied using a large
data sample of 67 fb^{-1} accumulated with the Belle detector at the KEKB
asymmetric e^+e^- collider. We have measured the cross section for the process
gamma gamma -> K^+ K^- for center-of-mass energies between 1.4 and 2.4 GeV, and
found three new resonant structures in the energy region between 1.6 and 2.4
GeV. The angular differential cross sections have also been measured.Comment: 24 pages, 8 figures, to appear in Euro. Phys. Jour.
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