Polycomb-Mediated Loss of miR-31 Activates NIK-Dependent NF-κB Pathway in Adult T Cell Leukemia and Other Cancers

SummaryConstitutive NF-κB activation has causative roles in adult T cell leukemia (ATL) caused by HTLV-1 and other cancers. Here, we report a pathway involving Polycomb-mediated miRNA silencing and NF-κB activation. We determine the miRNA signatures and reveal miR-31 loss in primary ATL cells. MiR-31 negatively regulates the noncanonical NF-κB pathway by targeting NF-κB inducing kinase (NIK). Loss of miR-31 therefore triggers oncogenic signaling. In ATL cells, miR-31 level is epigenetically regulated, and aberrant upregulation of Polycomb proteins contribute to miR-31 downregulation in an epigenetic fashion, leading to activation of NF-κB and apoptosis resistance. Furthermore, this emerging circuit operates in other cancers and receptor-initiated NF-κB cascade. Our findings provide a perspective involving the epigenetic program, inflammatory responses, and oncogenic signaling

World squid fisheries

Peer reviewedPublisher PD

The need for building role models for motorcycle riders' education in the kingdom of Cambodia

In Cambodia, a rider's license is not required for motorcycles below 125 cc. and 73% of traffic accident fatalities among persons aged 15–19 years involve unlicensed riders. Motorcycle riding differs from automobile driving in that motorcycle riding reflects the rider's skill to a much greater degree, so unskilled riding is much more highly correlated with accidents. Going forward, in addition to gaining knowledge from traffic safety education, motorcycle riders should also learn basic specific motorcycle techniques. In particular, having riders learn and practice the three key tasks in motorcycle riding (breaking, leaning, and exiting) is effective at curbing the number of motorcycle accidents. In a new initiative aimed at improving riding skill, university students, instructors, and police officers were given practical riding instruction on a course using pylons. Program participants were able to learn riding techniques in an enjoyable setting, while seeing the potential for becoming motorcycle riding role models. Keywords: Motorcycles, Driving skills, Practical training, Role models, Educatio

Primary pulmonary leiomyosarcoma: report of a case diagnosed by fine needle aspiration cytology

Primary pulmonary leiomyosarcoma is a rare but important entity. We report a case diagnosed by fine needle aspiration cytology. CASE: A 73-year-old male presented with an asymptomatic, right, pulmonary, subpleural nodule detected by computed tomography during follow-up for chronic obstructive pulmonary disease. Fine needle aspiration cytology showed cellular smears with numerous single or loosely cohesive groups of spindle-shaped to round cells. The tumor cell nuclei were blunt ended (cigar shaped), with fine to fine-granular chromatin, prominent nucleoli and an irregular nuclear rim. The tumor cells were positive for desmin and negative for cytokeratin and S-100 protein by immunocytochemistry. Right upper lobectomy with lymph node dissection was performed. Pathologic diagnosis after microscopic, immunohistochemical and electron microscopic studies was leiomyosarcoma. CONCLUSION: To our knowledge, this is the first reported case of primary pulmonary leiomyosarcoma arising in the subpleural region diagnosed by fine needle aspiration cytology. Immunocytochemistry was useful in establishing the diagnosis in this case