Understanding plurality in soil fertility knowledge for enhanced science-farmer communication a case study in Kenya

Sustainable soil management underpins many of the UN Sustainable Development Goals (SDGs). Knowledge exchange between farmers and soil scientists or extension officers is essential to create hybrid knowledge for sustainable soil fertility management under climate change. However, miscommunication between them and the unequal distribution of information reduce both the absorption of information and its implementation for soil management. Studies that provide thorough examinations of farmers’ knowledge systems about soil fertility, the effect of the difference of content and sources of information, and the effects of external and internal factors, including determinants of intentions for farmers’ soil knowledge construction and its implementation, are still rare. Therefore, this study’s aim was to explain how the construction of farmers’ knowledge of soil fertility compares to soil scientific knowledge of fertility and how the farmers’ knowledge system is used for land management. The aim is divided into three main objectives: to examine the similarities and differences between farmers’ qualitative evaluation and soil science quantitative analysis for soil fertility classification; to explore farmers’ and scientists’ mental models of soil fertility and the relationship between soil properties and processes for communicating soil knowledge; and to explore how cropping and land management responses to climate variability and soil type are shaped by perception of risk, individual motivation and perceived capacity. Empirical fieldwork was carried out in two villages in Kitui County, Kenya, in 2016 to compare the effect of location on the construction and implementation of soil knowledge. Structured questionnaire interviews, participatory methods and focus group discussion involving 60 farmers, two extension officers and eight soil scientists (in Wageningen) were used for social and soil knowledge data collection. 116 soil samples were taken from the places with the most and worst fertile soils from farmers’ fields, according to the farmers’ perceptions, and their physiochemical parameters were analysed. This study revealed the importance of soil texture and colour in both soil scientific and local soil classification: although the farmers did not use scientific methods or terminology, they were aware of a significant relationship between the amount of nutrients and water holding capacity (WHC). The location of soils and previous soil degradation affected the farmers’ knowledge construction. The mental models clearly showed that farmers’ perception of soil fertility was focused on WHC, manure and fertilizer application, and location. Different perceptions of soil processes also appeared, both among farmers and between farmers and soil scientists. Farmers’ crop selection and timing of seeding differed according to livelihood strategy, soil type, water availability and the occurrence of drought events. Communication with extension officers increased their adoption of innovations. Based on the results, this study recommended the development of a hybrid communication approach in order to co-learn with farmers and accelerate communication among stakeholders, with the goal of creating hybrid knowledge for site-specific sustainable soil management

Comparing farmers’ qualitative evaluation of soil fertility with quantitative soil fertility indicators in Kitui County, Kenya

Soil fertility is vital for agricultural productivity, yet poor soils and erosion remain a management challenge in many parts of sub-Saharan Africa. One challenge is that soil scientists and farmers often evaluate soil fertility using different knowledge systems and the implications have not been clearly reconciled within the literature. In particular, whether farmers are observing similar aspects of structure and function as classified in soil science. If so, what can we learn about how soil fertility is evaluated and communicated in terms of developing a hybrid approach that improves communication of ideas between different stakeholders. This paper addresses this challenge by examining the similarities and differences between farmers’ qualitative evaluation and soil science quantitative analysis for soil fertility classification, and how location of soils influence farmers’ evaluation of soil fertility. Empirical fieldwork was carried out in two villages in Kitui County, Kenya with 60 farmers using semi-structured interviews and focus group discussion. Based on farmer perception, 116 soil samples of the best and worst soil fertility taken and analysed for physiochemical factors. Farmers had a consistent classification system and primarily relied on texture and colour as indicators for good soil fertility and texture alone for poor soils. Soils with fine texture under the local semi-arid climate were associated with higher pH, TOC and WHC and fertile black and red soils were associated with pH, TOC, WHC and AP based on differences in bed rock. Poor soil fertility was associated with sandy soils and soils with no colour in their local name. Spatial location is an important consideration in farmers’ evaluations, reflecting awareness of local diversity in soil and historical social or environmental factors. Local historical narratives reveal the importance in changes to humus, consistent with technical knowledge about the role of soil organic matter for soil fertility. The paper provides better understanding of farmers’ soil classification, evaluation processes and perspectives that help to inform scientists working with alternative frameworks for assessment and, in doing so, supports the development of local tailor-made soil assessment systems

ドメイン蛋白質のコンホメーション安定性とコロイド安定性解析による抗体凝集機構の解明

学位の種別: 課程博士審査委員会委員 : （主査）東京大学客員教授 本田 真也, 東京大学教授 上田 卓也, 東京大学教授 伊藤 耕一, 東京大学准教授 深井 周也, 東京大学准教授 新井 宗仁University of Tokyo(東京大学

The interaction of IQGAP1 with the exocyst complex is required for tumor cell invasion downstream of Cdc42 and RhoA

Invadopodia are actin-based membrane protrusions formed at contact sites between invasive tumor cells and the extracellular matrix with matrix proteolytic activity. Actin regulatory proteins participate in invadopodia formation, whereas matrix degradation requires metalloproteinases (MMPs) targeted to invadopodia. In this study, we show that the vesicle-tethering exocyst complex is required for matrix proteolysis and invasion of breast carcinoma cells. We demonstrate that the exocyst subunits Sec3 and Sec8 interact with the polarity protein IQGAP1 and that this interaction is triggered by active Cdc42 and RhoA, which are essential for matrix degradation. Interaction between IQGAP1 and the exocyst is necessary for invadopodia activity because enhancement of matrix degradation induced by the expression of IQGAP1 is lost upon deletion of the exocyst-binding site. We further show that the exocyst and IQGAP1 are required for the accumulation of cell surface membrane type 1 MMP at invadopodia. Based on these results, we propose that invadopodia function in tumor cells relies on the coordination of cytoskeletal assembly and exocytosis downstream of Rho guanosine triphosphatases

Exo70-Mediated Recruitment of Nucleoporin Nup62 at the Leading Edge of Migrating Cells is Required for Cell Migration

Nucleoporin Nup62 localizes at the central channel of the nuclear pore complex and is essential for nucleocytoplasmic transport. Through its FG-repeat domain, Nup62 regulates nuclear pore permeability and binds nuclear transport receptors. Here, we report that Nup62 interacts directly with Exo70 and colocalizes with Exo70 at the leading edge of migrating cells. Nup62 binds the N-terminal domain of Exo70 through its coiled-coil domain but not through its FG-repeat domain. Selective inhibition of leading edge Nup62 using RNA interference significantly reduces cell migration. Furthermore, Exo70 recruits Nup62 at the plasma membrane and at filopodia. Removal of the Exo70-binding domain of Nup62 prevents leading edge localization of Nup62. Analogous to Exo70, Nup62 cycles between the plasma membrane and the perinuclear recycling compartment. Altogether, we propose that Nup62 not solely regulates access to the cell nucleus, but additionally functions in conjunction with Exo70, a key regulator of exocytosis and actin dynamics, at the leading edge of migrating cells

Rolly Protein (ROLP)-Epb4.1/3: A Potential Protein-Protein Interaction Relevant for the Maintenance of Cell Adhesion

We recently described Rolly Protein (ROLP), a small protein synthesized by substrate-adherent cells in a broad range of tissues. In a first set of experiments performed taking advantage of bone forming tibial cartilage as an experimental model we showed that ROLP transcription is associated to cells in an active proliferation state, whereas its downregulation is observed when cell proliferation decreases. Taking advantage of siRNA technology we also documented the expression modulation of some apoptosis-related genes in ROLP-silenced cells. In this work we search for the possible molecular interactors of ROLP by using both the antibody array approach as well as the co-immunoprecipitation approach. Results suggest the occurrence of an interaction of ROLP with Erythrocyte membrane Protein Band 4.1/3 (Epb4.1/3), an oncosuppressor downregulated in tumor development and in metastatic tissues; in addition we report experimental results that keep in line also with a potential interaction of ROLP with other PDZ-containing proteins. We also present experimental evidences supporting a role played by ROLP in cell adhesion thus supporting the existence of a biologically relevant link between ROLP and Epb4.1/3. We here suggest that ROLP might exert its biological role cooperating with Epb4.1/3, a protein that is involved in biological pathways that are often inhibited in tumor metastasis. Given the role of Epb4.1/3 in contrasting cancerogenesis we think that its cooperation with ROLP might be relevant in cancer studies and deserves further investigation

Interleukin-17D and Nrf2 mediate initial innate immune cell recruitment and restrict MCMV infection.

Innate immune cells quickly infiltrate the site of pathogen entry and not only stave off infection but also initiate antigen presentation and promote adaptive immunity. The recruitment of innate leukocytes has been well studied in the context of extracellular bacterial and fungal infection but less during viral infections. We have recently shown that the understudied cytokine Interleukin (IL)-17D can mediate neutrophil, natural killer (NK) cell and monocyte infiltration in sterile inflammation and cancer. Herein, we show that early immune cell accumulation at the peritoneal site of infection by mouse cytomegalovirus (MCMV) is mediated by IL-17D. Mice deficient in IL-17D or the transcription factor Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), an inducer of IL-17D, featured an early decreased number of innate immune cells at the point of viral entry and were more susceptible to MCMV infection. Interestingly, we were able to artificially induce innate leukocyte infiltration by applying the Nrf2 activator tert-butylhydroquinone (tBHQ), which rendered mice less susceptible to MCMV infection. Our results implicate the Nrf2/IL-17D axis as a sensor of viral infection and suggest therapeutic benefit in boosting this pathway to promote innate antiviral responses

Identification of two novel breast cancer loci through large-scale genome-wide association study in the Japanese population

Genome-wide association studies (GWAS) have successfully identified about 70 genomic loci associated with breast cancer. Owing to the complexity of linkage disequilibrium and environmental exposures in different populations, it is essential to perform regional GWAS for better risk prediction. This study aimed to investigate the genetic architecture and to assess common genetic risk model of breast cancer with 6,669 breast cancer patients and 21,930 female controls in the Japanese population. This GWAS identified 11 genomic loci that surpass genome-wide significance threshold of P < 5.0 × 10−8 with nine previously reported loci and two novel loci that include rs9862599 on 3q13.11 (ALCAM) and rs75286142 on 21q22.12 (CLIC6-RUNX1). Validation study was carried out with 981 breast cancer cases and 1,394 controls from the Aichi Cancer Center. Pathway analyses of GWAS signals identified association of dopamine receptor medicated signaling and protein amino acid deacetylation with breast cancer. Weighted genetic risk score showed that individuals who were categorized in the highest risk group are approximately 3.7 times more likely to develop breast cancer compared to individuals in the lowest risk group. This well-powered GWAS is a representative study to identify SNPs that are associated with breast cancer in the Japanese population